Antisera to bovine oligodendroglia raised in guinea pigs bind to surface of rat oligodendroglia and Schwann cells

R. P. Lisak, Rebecca M. Pruss, P. G E Kennedy, Oded Abramsky, David E Pleasure, D. H. Silberberg

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Antisera against bulk isolated bovine oligodendrocytes was raised in 2 guinea pigs (GPaBO). The sera bound only to surface of rat oligodendrocytes in dissociated rat corpus callosum and cerebellar cultures and only to surface of Schwann cells in primary and secondary sciatic nerve and dorsal root ganglia cultures as determined by indirect immunofluorescence. No cells in muscle or retinal cultures bound the guinea pig antisera. The shared antigen does not seem to be either galactocerebroside (GalC) or a myelin basic protein. GPaBO can serve as a useful marker in double-label experiments to identify oligodendrocytes and Schwann cells in dissociated cultures.

Original languageEnglish (US)
Pages (from-to)119-124
Number of pages6
JournalNeuroscience Letters
Volume17
Issue number1-2
DOIs
StatePublished - 1980
Externally publishedYes

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Schwann Cells
Oligodendroglia
Immune Sera
Guinea Pigs
Myelin Basic Protein
Corpus Callosum
Spinal Ganglia
Sciatic Nerve
Indirect Fluorescent Antibody Technique
Muscle Cells
Antigens
Serum

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Antisera to bovine oligodendroglia raised in guinea pigs bind to surface of rat oligodendroglia and Schwann cells. / Lisak, R. P.; Pruss, Rebecca M.; Kennedy, P. G E; Abramsky, Oded; Pleasure, David E; Silberberg, D. H.

In: Neuroscience Letters, Vol. 17, No. 1-2, 1980, p. 119-124.

Research output: Contribution to journalArticle

Lisak, R. P. ; Pruss, Rebecca M. ; Kennedy, P. G E ; Abramsky, Oded ; Pleasure, David E ; Silberberg, D. H. / Antisera to bovine oligodendroglia raised in guinea pigs bind to surface of rat oligodendroglia and Schwann cells. In: Neuroscience Letters. 1980 ; Vol. 17, No. 1-2. pp. 119-124.
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