TY - JOUR
T1 - Antisera to a synthetic peptide recognize native and denatured rat estrogen receptors
AU - Furlow, John
AU - Ahrens, Helga
AU - Mueller, Gerald C.
AU - Gorski, Jack
PY - 1990/1/1
Y1 - 1990/1/1
N2 - Specific polyclonal antisera to the rat estrogen receptor (rER) were developed using a synthetic peptide as the antigen. The peptide corresponds to amino acids 270-284 deduced from the cloned rER cDNA and has no homology to other steroid hormone receptors. Antipeptide antisera raised in rabbits specifically recognize a 67, 000 mol wt protein, shown to be the rER, in Western blot experiments. In immunoprecipitation experiments, one tested antiserum bound unoccupied as well as 17β-estradiol-occupied rERs, indicating that this region is exposed in both receptor forms. This antiserum shows no crossreactivity with rat progesterone or glucocorticoid receptors. Cross-reactivity with rabbit, human, and, to a lesser extent, bovine ERs was observed.
AB - Specific polyclonal antisera to the rat estrogen receptor (rER) were developed using a synthetic peptide as the antigen. The peptide corresponds to amino acids 270-284 deduced from the cloned rER cDNA and has no homology to other steroid hormone receptors. Antipeptide antisera raised in rabbits specifically recognize a 67, 000 mol wt protein, shown to be the rER, in Western blot experiments. In immunoprecipitation experiments, one tested antiserum bound unoccupied as well as 17β-estradiol-occupied rERs, indicating that this region is exposed in both receptor forms. This antiserum shows no crossreactivity with rat progesterone or glucocorticoid receptors. Cross-reactivity with rabbit, human, and, to a lesser extent, bovine ERs was observed.
UR - http://www.scopus.com/inward/record.url?scp=0025002573&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025002573&partnerID=8YFLogxK
U2 - 10.1210/endo-127-3-1028
DO - 10.1210/endo-127-3-1028
M3 - Article
C2 - 2201530
AN - SCOPUS:0025002573
VL - 127
SP - 1028
EP - 1032
JO - Endocrinology
JF - Endocrinology
SN - 0013-7227
IS - 3
ER -