Antisera to a synthetic peptide recognize native and denatured rat estrogen receptors

John Furlow, Helga Ahrens, Gerald C. Mueller, Jack Gorski

Research output: Contribution to journalArticle

87 Scopus citations

Abstract

Specific polyclonal antisera to the rat estrogen receptor (rER) were developed using a synthetic peptide as the antigen. The peptide corresponds to amino acids 270-284 deduced from the cloned rER cDNA and has no homology to other steroid hormone receptors. Antipeptide antisera raised in rabbits specifically recognize a 67, 000 mol wt protein, shown to be the rER, in Western blot experiments. In immunoprecipitation experiments, one tested antiserum bound unoccupied as well as 17β-estradiol-occupied rERs, indicating that this region is exposed in both receptor forms. This antiserum shows no crossreactivity with rat progesterone or glucocorticoid receptors. Cross-reactivity with rabbit, human, and, to a lesser extent, bovine ERs was observed.

Original languageEnglish (US)
Pages (from-to)1028-1032
Number of pages5
JournalEndocrinology
Volume127
Issue number3
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology

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