Antisense knockdown of the β1 integrin subunit in the chicken embryo results in abnormal neural crest cell development

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Neural crest cells escape the neural tube by undergoing an epithelial to mesenchymal transition (EMT). This is followed by extensive migration along specific pathways that are lined with extracellular matrix (ECM). In this study, we have examined the roles of matrix receptors containing β1 integrin subunits in neural crest cell morphogenesis using antisense morpholino oligos electroporated in ovo into avian neural crest cell precursors. Our results show that reduced levels of expression of β1 integrin subunits in the dorsal neural tube results in an abnormal epithelial to mesenchymal transition. In approximately half of the experimental embryos, however, some neural crest cells filled with β1 antisense are able to escape the neural tube and migrate ventrally, indicating that grossly normal migration of trunk neural crest cells can take place after β1 integrin expression is reduced. This study shows the potential of this novel method for investigating the roles of genes that are required for the survival of early mouse embryos in later development events.

Original languageEnglish (US)
Pages (from-to)1135-1139
Number of pages5
JournalInternational Journal of Biochemistry and Cell Biology
Volume36
Issue number6
DOIs
StatePublished - Jun 2004

Keywords

  • Electroporation
  • Epithelial to mesenchymal transition
  • Extracellular matrix
  • In vivo
  • Morpholino

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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