Antisense galectin-3 alters thymidine incorporation in human MDA-MB435 breast cancer cells

Frédéric A. Van den Brûle, Akeila Bellahcene, Pascale Jackers, Fu-Tong Liu, Mark E. Sobel, Vincent Castronovo

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Galectin-3 is a 30-kDa galactose-binding protein member of the galectin family. Galectin-3 is involved in multiple intracellular and extracellular biological functions, e.g. interactions with laminin and with nucleic acids. This latter property is consistent with the presence of a serum-response factor-like domain at the amino-terminal part of the protein. Galectin-3 expression is upregulated during serum-mediated induction of proliferation. In order to examine the role of galectin-3 in breast cancer cell proliferation, we examined in this study the influence of antisense galectin-3 complementary DNA stable transfection on the in vitro thymidine incorporation of human breast cancer MDA-MB435 cells. Two stable transfectants, clones 5.24 and 5.29, were selected based both on the presence of a complete CMV promoter-antisense galectin-3 cDNA cassette as assayed by polymerase chain reaction, and on efficient down-regulation of galectin-3 protein expression as determined by Western blotting. Thymidine incorporation experiments showed that both clones were characterized by significantly decreased values of DNA incorporation compared to wild-type transfectants (55 to 68%, and 71 to 82% of the control clone values). Our data demonstrate for the first time that galectin-3 decreases thymidine incorporation in breast cancer cells. The mechanism underlying this property of galectin-3 and its importance during breast cancer development remain to be elucidated.

Original languageEnglish (US)
Pages (from-to)261-264
Number of pages4
JournalInternational Journal of Oncology
Volume11
Issue number2
StatePublished - 1997

Fingerprint

Galectin 3
Thymidine
Breast Neoplasms
Clone Cells
Complementary DNA
Serum Response Factor
Galectins
Laminin
Nucleic Acids
Transfection
Proteins
Down-Regulation
Western Blotting
Cell Proliferation
Polymerase Chain Reaction

Keywords

  • Antisense
  • Breast cancer
  • Galectin-3
  • Proliferation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Van den Brûle, F. A., Bellahcene, A., Jackers, P., Liu, F-T., Sobel, M. E., & Castronovo, V. (1997). Antisense galectin-3 alters thymidine incorporation in human MDA-MB435 breast cancer cells. International Journal of Oncology, 11(2), 261-264.

Antisense galectin-3 alters thymidine incorporation in human MDA-MB435 breast cancer cells. / Van den Brûle, Frédéric A.; Bellahcene, Akeila; Jackers, Pascale; Liu, Fu-Tong; Sobel, Mark E.; Castronovo, Vincent.

In: International Journal of Oncology, Vol. 11, No. 2, 1997, p. 261-264.

Research output: Contribution to journalArticle

Van den Brûle, FA, Bellahcene, A, Jackers, P, Liu, F-T, Sobel, ME & Castronovo, V 1997, 'Antisense galectin-3 alters thymidine incorporation in human MDA-MB435 breast cancer cells', International Journal of Oncology, vol. 11, no. 2, pp. 261-264.
Van den Brûle FA, Bellahcene A, Jackers P, Liu F-T, Sobel ME, Castronovo V. Antisense galectin-3 alters thymidine incorporation in human MDA-MB435 breast cancer cells. International Journal of Oncology. 1997;11(2):261-264.
Van den Brûle, Frédéric A. ; Bellahcene, Akeila ; Jackers, Pascale ; Liu, Fu-Tong ; Sobel, Mark E. ; Castronovo, Vincent. / Antisense galectin-3 alters thymidine incorporation in human MDA-MB435 breast cancer cells. In: International Journal of Oncology. 1997 ; Vol. 11, No. 2. pp. 261-264.
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