Antisense galectin-3 alters thymidine incorporation in human MDA-MB435 breast cancer cells

Frédéric A. Van den Brûle, Akeila Bellahcene, Pascale Jackers, Fu-Tong Liu, Mark E. Sobel, Vincent Castronovo

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Galectin-3 is a 30-kDa galactose-binding protein member of the galectin family. Galectin-3 is involved in multiple intracellular and extracellular biological functions, e.g. interactions with laminin and with nucleic acids. This latter property is consistent with the presence of a serum-response factor-like domain at the amino-terminal part of the protein. Galectin-3 expression is upregulated during serum-mediated induction of proliferation. In order to examine the role of galectin-3 in breast cancer cell proliferation, we examined in this study the influence of antisense galectin-3 complementary DNA stable transfection on the in vitro thymidine incorporation of human breast cancer MDA-MB435 cells. Two stable transfectants, clones 5.24 and 5.29, were selected based both on the presence of a complete CMV promoter-antisense galectin-3 cDNA cassette as assayed by polymerase chain reaction, and on efficient down-regulation of galectin-3 protein expression as determined by Western blotting. Thymidine incorporation experiments showed that both clones were characterized by significantly decreased values of DNA incorporation compared to wild-type transfectants (55 to 68%, and 71 to 82% of the control clone values). Our data demonstrate for the first time that galectin-3 decreases thymidine incorporation in breast cancer cells. The mechanism underlying this property of galectin-3 and its importance during breast cancer development remain to be elucidated.

Original languageEnglish (US)
Pages (from-to)261-264
Number of pages4
JournalInternational Journal of Oncology
Volume11
Issue number2
StatePublished - 1997

Keywords

  • Antisense
  • Breast cancer
  • Galectin-3
  • Proliferation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Fingerprint Dive into the research topics of 'Antisense galectin-3 alters thymidine incorporation in human MDA-MB435 breast cancer cells'. Together they form a unique fingerprint.

  • Cite this

    Van den Brûle, F. A., Bellahcene, A., Jackers, P., Liu, F-T., Sobel, M. E., & Castronovo, V. (1997). Antisense galectin-3 alters thymidine incorporation in human MDA-MB435 breast cancer cells. International Journal of Oncology, 11(2), 261-264.