Antisense galectin-3 alters thymidine incorporation in human MDA-MB435 breast cancer cells

Frédéric A. Van den Brûle, Akeila Bellahcene, Pascale Jackers, Fu-Tong Liu, Mark E. Sobel, Vincent Castronovo

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Galectin-3 is a 30-kDa galactose-binding protein member of the galectin family. Galectin-3 is involved in multiple intracellular and extracellular biological functions, e.g. interactions with laminin and with nucleic acids. This latter property is consistent with the presence of a serum-response factor-like domain at the amino-terminal part of the protein. Galectin-3 expression is upregulated during serum-mediated induction of proliferation. In order to examine the role of galectin-3 in breast cancer cell proliferation, we examined in this study the influence of antisense galectin-3 complementary DNA stable transfection on the in vitro thymidine incorporation of human breast cancer MDA-MB435 cells. Two stable transfectants, clones 5.24 and 5.29, were selected based both on the presence of a complete CMV promoter-antisense galectin-3 cDNA cassette as assayed by polymerase chain reaction, and on efficient down-regulation of galectin-3 protein expression as determined by Western blotting. Thymidine incorporation experiments showed that both clones were characterized by significantly decreased values of DNA incorporation compared to wild-type transfectants (55 to 68%, and 71 to 82% of the control clone values). Our data demonstrate for the first time that galectin-3 decreases thymidine incorporation in breast cancer cells. The mechanism underlying this property of galectin-3 and its importance during breast cancer development remain to be elucidated.

Original languageEnglish (US)
Pages (from-to)261-264
Number of pages4
JournalInternational Journal of Oncology
Issue number2
StatePublished - 1997


  • Antisense
  • Breast cancer
  • Galectin-3
  • Proliferation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology


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