TY - JOUR
T1 - Antioxidant supplementation reduces genomic aberrations in human induced pluripotent stem cells
AU - Ji, Junfeng
AU - Sharma, Vivek
AU - Qi, Suxia
AU - Guarch, Meritxell Espino
AU - Zhao, Ping
AU - Luo, Zhiwei
AU - Fan, Wenxia
AU - Wang, Yu
AU - Mbabaali, Faridah
AU - Neculai, Dante
AU - Esteban, Miguel Angel
AU - Mcpherson, John Douglas
AU - Batada, Nizar N.
PY - 2014/1/14
Y1 - 2014/1/14
N2 - Somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) using oncogenic transcription factors. However, this method leads to genetic aberrations in iPSCs via unknown mechanisms, which may limit their clinical use. Here, we demonstrate that the supplementation of growth media with antioxidants reduces the genome instability of cells transduced with the reprogramming factors. Antioxidant supplementation did not affect transgene expression level or silencing kinetics. Importantly, iPSCs made with antioxidants had significantly fewer de novo copy number variations, but not fewer coding point mutations, than iPSCs made without antioxidants. Our results suggest that the quality and safety of human iPSCs might be enhanced by using antioxidants in the growth media during the generation and maintenance of iPSCs.
AB - Somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) using oncogenic transcription factors. However, this method leads to genetic aberrations in iPSCs via unknown mechanisms, which may limit their clinical use. Here, we demonstrate that the supplementation of growth media with antioxidants reduces the genome instability of cells transduced with the reprogramming factors. Antioxidant supplementation did not affect transgene expression level or silencing kinetics. Importantly, iPSCs made with antioxidants had significantly fewer de novo copy number variations, but not fewer coding point mutations, than iPSCs made without antioxidants. Our results suggest that the quality and safety of human iPSCs might be enhanced by using antioxidants in the growth media during the generation and maintenance of iPSCs.
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U2 - 10.1016/j.stemcr.2013.11.004
DO - 10.1016/j.stemcr.2013.11.004
M3 - Article
C2 - 24511469
AN - SCOPUS:84892598278
VL - 2
SP - 44
EP - 51
JO - Stem Cell Reports
JF - Stem Cell Reports
SN - 2213-6711
IS - 1
ER -