Antioxidant supplementation reduces genomic aberrations in human induced pluripotent stem cells

Junfeng Ji, Vivek Sharma, Suxia Qi, Meritxell Espino Guarch, Ping Zhao, Zhiwei Luo, Wenxia Fan, Yu Wang, Faridah Mbabaali, Dante Neculai, Miguel Angel Esteban, John Douglas Mcpherson, Nizar N. Batada

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

Somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs) using oncogenic transcription factors. However, this method leads to genetic aberrations in iPSCs via unknown mechanisms, which may limit their clinical use. Here, we demonstrate that the supplementation of growth media with antioxidants reduces the genome instability of cells transduced with the reprogramming factors. Antioxidant supplementation did not affect transgene expression level or silencing kinetics. Importantly, iPSCs made with antioxidants had significantly fewer de novo copy number variations, but not fewer coding point mutations, than iPSCs made without antioxidants. Our results suggest that the quality and safety of human iPSCs might be enhanced by using antioxidants in the growth media during the generation and maintenance of iPSCs.

Original languageEnglish (US)
Pages (from-to)44-51
Number of pages8
JournalStem Cell Reports
Volume2
Issue number1
DOIs
StatePublished - Jan 14 2014
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Developmental Biology
  • Genetics
  • Medicine(all)

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    Ji, J., Sharma, V., Qi, S., Guarch, M. E., Zhao, P., Luo, Z., Fan, W., Wang, Y., Mbabaali, F., Neculai, D., Esteban, M. A., Mcpherson, J. D., & Batada, N. N. (2014). Antioxidant supplementation reduces genomic aberrations in human induced pluripotent stem cells. Stem Cell Reports, 2(1), 44-51. https://doi.org/10.1016/j.stemcr.2013.11.004