Antimitochondrial (pyruvate dehydrogenase) antibodies in leprosy

B. Gilburd, L. Ziporen, D. Zharhary, M. Blank, N. Zurgil, M. A. Scheinberg, L. H. Guedes, M. Eric Gershwin, Y. Shoenfeld

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Abstract

Sera from 69 patients with leprosy but without liver involvement were assayed for the presence of mitochondrial pyruvate dehydrogenase (PDH)-specific autoantibodies by enzyme-linked immunoabsorbent assay (ELISA), immunoblotting using PDH as an antigen and by enzymatic inhibition test. Twenty-seven of the leprosy serum samples (39.1%) were found to react with PDH by ELISA. However, unlike sera from primary biliary cirrhosis (PBC) patients, none of these were able to inhibit the PDH enzymatic activity. By immunoblotting, it was found that only 2 of the 27 positive sera recognized the 74-kD protein of the PDH complex, which is recognized by sera of most PBC patients. The antimitochondrial antibodies in lepra most probably recognize different epitopes than those in PBC. These findings may indicate that anti-PDH autoantibodies in patients with leprosy may arise by polyclonal B cell stimulation and may represent natural anti-PDH autoantibodies.

Original languageEnglish (US)
Pages (from-to)14-19
Number of pages6
JournalJournal of Clinical Immunology
Volume14
Issue number1
DOIs
StatePublished - Jan 1994

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Leprosy
Pyruvic Acid
Oxidoreductases
Biliary Liver Cirrhosis
Antibodies
Autoantibodies
Serum
Immunoblotting
Pyruvate Dehydrogenase Complex
Enzymes
Epitopes
B-Lymphocytes
Antigens
Liver
Proteins

Keywords

  • anti-pyruvate dehydrogenase
  • Antimitochondrial antibodies
  • leprosy
  • primary biliary cirrhosis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Gilburd, B., Ziporen, L., Zharhary, D., Blank, M., Zurgil, N., Scheinberg, M. A., ... Shoenfeld, Y. (1994). Antimitochondrial (pyruvate dehydrogenase) antibodies in leprosy. Journal of Clinical Immunology, 14(1), 14-19. https://doi.org/10.1007/BF01541171

Antimitochondrial (pyruvate dehydrogenase) antibodies in leprosy. / Gilburd, B.; Ziporen, L.; Zharhary, D.; Blank, M.; Zurgil, N.; Scheinberg, M. A.; Guedes, L. H.; Gershwin, M. Eric; Shoenfeld, Y.

In: Journal of Clinical Immunology, Vol. 14, No. 1, 01.1994, p. 14-19.

Research output: Contribution to journalArticle

Gilburd, B, Ziporen, L, Zharhary, D, Blank, M, Zurgil, N, Scheinberg, MA, Guedes, LH, Gershwin, ME & Shoenfeld, Y 1994, 'Antimitochondrial (pyruvate dehydrogenase) antibodies in leprosy', Journal of Clinical Immunology, vol. 14, no. 1, pp. 14-19. https://doi.org/10.1007/BF01541171
Gilburd B, Ziporen L, Zharhary D, Blank M, Zurgil N, Scheinberg MA et al. Antimitochondrial (pyruvate dehydrogenase) antibodies in leprosy. Journal of Clinical Immunology. 1994 Jan;14(1):14-19. https://doi.org/10.1007/BF01541171
Gilburd, B. ; Ziporen, L. ; Zharhary, D. ; Blank, M. ; Zurgil, N. ; Scheinberg, M. A. ; Guedes, L. H. ; Gershwin, M. Eric ; Shoenfeld, Y. / Antimitochondrial (pyruvate dehydrogenase) antibodies in leprosy. In: Journal of Clinical Immunology. 1994 ; Vol. 14, No. 1. pp. 14-19.
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