Antimicrobial properties of the chelating agent EDTA on streptococcal bovine mastitis isolates

Jeffrey S. Reidmiller, Wayne L. Smith, Mary M. Sawyer, Bennie Osburn, Jeffrey L Stott, James S Cullor

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


To determine the efficacy of the chelating agent EDTA on microbial growth, separate cultures of two streptocoocal bovine mastitis isolates, Streptococcus agalactiae and Streptococcus uberis, were exposed to known concentrations of EDTA. Bacterial cultures of 108 CFU/ml were exposed to concentrations of EDTA ranging from 30 to 100 mM in an in-vitro-milk environment. Multiple replications of cultures exposed to EDTA were plated during a two-hour time course. A concentration of 100 mM EDTA resulted in a 90% reduction of S. agalactiae and a 99% reduction of S. uberis. Under these experimental conditions, EDTA treatments in cultures of both isolates exhibited from 1 to 2 log reductions suggesting that EDTA is a potentially effective antimicrobial against streptococcal isolates implicated in causing bovine mastitis.

Original languageEnglish (US)
Pages (from-to)1460-1462
Number of pages3
JournalJournal of Food Protection
Issue number6
StatePublished - Jun 2006

ASJC Scopus subject areas

  • Food Science
  • Applied Microbiology and Biotechnology
  • Biotechnology


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