To determine the efficacy of the chelating agent EDTA on microbial growth, separate cultures of two streptocoocal bovine mastitis isolates, Streptococcus agalactiae and Streptococcus uberis, were exposed to known concentrations of EDTA. Bacterial cultures of 108 CFU/ml were exposed to concentrations of EDTA ranging from 30 to 100 mM in an in-vitro-milk environment. Multiple replications of cultures exposed to EDTA were plated during a two-hour time course. A concentration of 100 mM EDTA resulted in a 90% reduction of S. agalactiae and a 99% reduction of S. uberis. Under these experimental conditions, EDTA treatments in cultures of both isolates exhibited from 1 to 2 log reductions suggesting that EDTA is a potentially effective antimicrobial against streptococcal isolates implicated in causing bovine mastitis.
|Original language||English (US)|
|Number of pages||3|
|Journal||Journal of Food Protection|
|State||Published - Jun 2006|
ASJC Scopus subject areas
- Food Science
- Applied Microbiology and Biotechnology