Antimicrobial polypeptides of the human colonic epithelium

Scott J. Howell, Dennis Wilk, Satya P. Yadav, Charles L Bevins

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

The lumen of the human colon is heavily colonized with microbes, but infections across its epithelial surface are infrequent. To address the hypothesis that antimicrobial polypeptides contribute to the barrier function of colonic epithelial cells, we examined cellular extracts from non-inflamed colonic mucosa using an antimicrobial assay. This approach yielded five polypeptides: three antimicrobials were previously identified as ribosomal polypeptides (L30, S19 and ubiquicidin), and two were members of the histone family (H1.5 and H2B). All exhibited bactericidal activity against Escherichia coli, and with the exception of S19, had been isolated by others based on their potent antimicrobial activity in other cells and tissues. These polypeptides normally reside inside cells and are proposed to contribute to the formation of the functional antimicrobial barrier of the colonic epithelium.

Original languageEnglish (US)
Pages (from-to)1763-1770
Number of pages8
JournalPeptides
Volume24
Issue number11
DOIs
StatePublished - Nov 2003
Externally publishedYes

Keywords

  • Antimicrobial peptide
  • Colon
  • Epithelia
  • Histone H1.5
  • Histone H2B
  • Ribosomal L30
  • Ribosomal S19
  • Ubiquicidin

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

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