Antimicrobial peptides in the stomach of Xenopus laevis

Karen S. Moore, Charles L Bevins, Michael M. Brasseur, Natale Tomassini, Kent Turner, Howard Eck, Michael Zasloff

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

Antimicrobial peptides are widely distributed in nature and appear to play a role in the host defense of plants and animals. In this study we report the existence of antimicrobial peptides in the stomach of the vertebrate Xenopus laevis, an animal previously shown to store high concentrations of antimicrobial peptides in its skin. Antimicrobial activity was detected in extracts of X. laevis stomach tissue and nine antimicrobial peptides were then purified. A novel 24-amino acid peptide, designated PGQ, was isolated from these extracts, and has the following amino acid sequence: GVLSNVIGYLKKLGTGALNAVLKQ. PGQ is relatively basic and has the potential to form an amphipathic α-helix. The other peptides isolated are members of the magainin family of antimicrobial peptides, and include magainins I and II, PGLa, xenopsin precursor fragment, and four caerulein precursor fragments. None of these peptides had been previously identified in tissues other than the skin. The purification of the peptides from stomach extracts and subsequent protein sequence analysis reveals that the peptides have undergone the same processing as their dermal counterparts, and that they are stored in their processed forms. Northern blot analysis indicates that the magainin family of peptides are synthesized in the stomach, and immunohistochemical studies demonstrate that magainin is stored in a novel granular multinucleated cell in the gastric mucosa of Xenopus. This study demonstrates that the magainin family of antimicrobial peptides is found in the gastrointestinal system of X. laevis and offers an opportunity to further define the physiological role of these defense peptides.

Original languageEnglish (US)
Pages (from-to)19851-19857
Number of pages7
JournalJournal of Biological Chemistry
Volume266
Issue number29
StatePublished - Oct 15 1991
Externally publishedYes

Fingerprint

Xenopus laevis
Stomach
Peptides
Magainins
Skin
Animals
Tissue
Ceruletide
Amino Acids
Protein Sequence Analysis
Xenopus
Gastric Mucosa
Northern Blotting
Purification
Vertebrates
Amino Acid Sequence

ASJC Scopus subject areas

  • Biochemistry

Cite this

Moore, K. S., Bevins, C. L., Brasseur, M. M., Tomassini, N., Turner, K., Eck, H., & Zasloff, M. (1991). Antimicrobial peptides in the stomach of Xenopus laevis. Journal of Biological Chemistry, 266(29), 19851-19857.

Antimicrobial peptides in the stomach of Xenopus laevis. / Moore, Karen S.; Bevins, Charles L; Brasseur, Michael M.; Tomassini, Natale; Turner, Kent; Eck, Howard; Zasloff, Michael.

In: Journal of Biological Chemistry, Vol. 266, No. 29, 15.10.1991, p. 19851-19857.

Research output: Contribution to journalArticle

Moore, KS, Bevins, CL, Brasseur, MM, Tomassini, N, Turner, K, Eck, H & Zasloff, M 1991, 'Antimicrobial peptides in the stomach of Xenopus laevis', Journal of Biological Chemistry, vol. 266, no. 29, pp. 19851-19857.
Moore KS, Bevins CL, Brasseur MM, Tomassini N, Turner K, Eck H et al. Antimicrobial peptides in the stomach of Xenopus laevis. Journal of Biological Chemistry. 1991 Oct 15;266(29):19851-19857.
Moore, Karen S. ; Bevins, Charles L ; Brasseur, Michael M. ; Tomassini, Natale ; Turner, Kent ; Eck, Howard ; Zasloff, Michael. / Antimicrobial peptides in the stomach of Xenopus laevis. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 29. pp. 19851-19857.
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