Antimicrobial effect of synergistic interaction between UV-A light and gallic acid against Escherichia coli O157: H7 in fresh produce wash water and biofilm

Andrea Cossu, Duygu Ercan, Qingyang Wang, Wendy Ann Peer, Nitin Nitin, Rohan V. Tikekar

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A synergistic interaction between gallic acid (GA) and UV-A light (UV-A + GA) to inactivate E. coli O157:H7 in spinach wash water and in biofilm was evaluated. A 30-min exposure to UV-A light in presence of 10 mM GA had relevant biological effect in the inactivation of E. coli O157:H7 in suspension in the absence (> 5 log(CFU/mL)) and the presence of organic content (> 3-log(CFU/mL) in 2000 mg O2/L COD (Chemical Oxygen Demand) organic load), and resulted in ~ 80% decrease in the metabolic activity of E. coli O157:H7 biofilm. GA solutions could be recycled through at least 3-cycles of UV-A treatment without a significant loss in antibacterial effect. Catalase reduced the extent of E. coli O157:H7 inactivation from the UV-A + GA treatment suggesting that generation of hydrogen peroxide was partially responsible for the observed antimicrobial effect. The UV-A + GA treatment was also found to be effective in causing > 3 log(CFU/mL) reductions in E. coli O157:H7 on the surface of spinach leaves. UV-A + GA treatment can serve as an effective intervention in the fresh produce sanitation. Industrial relevance The results of this study show that a synergistic interaction between gallic acid (GA) and UV-A (365 nm) light is an effective treatment for sanitation of fresh produce and water used to wash fresh produce. It was also found to be effective against the E.coli O157:H7 biofilm. The attractive attributes of this technology include a relatively low cost; specific, light-triggered activity; non-toxic nature and scalability. Thus, this technology has potential to replace conventional chemical sanitizer-based sanitation approaches.

Original languageEnglish (US)
Pages (from-to)44-52
Number of pages9
JournalInnovative Food Science and Emerging Technologies
Volume37
DOIs
StatePublished - Oct 1 2016

Fingerprint

Gallic Acid
Escherichia coli O157
fresh produce
Biofilms
anti-infective properties
Ultraviolet Rays
gallic acid
biofilm
Escherichia coli
Acids
Water
Sanitation
sanitation
water
acid treatment
Spinacia oleracea
inactivation
Ipomoea aquatica
Technology
Biological Oxygen Demand Analysis

Keywords

  • Biofilm
  • Escherichia coli O157:H7
  • Gallic acid
  • Photodynamic treatment
  • Photosensitization
  • Wash water

ASJC Scopus subject areas

  • Food Science
  • Chemistry(all)
  • Industrial and Manufacturing Engineering

Cite this

Antimicrobial effect of synergistic interaction between UV-A light and gallic acid against Escherichia coli O157 : H7 in fresh produce wash water and biofilm. / Cossu, Andrea; Ercan, Duygu; Wang, Qingyang; Peer, Wendy Ann; Nitin, Nitin; Tikekar, Rohan V.

In: Innovative Food Science and Emerging Technologies, Vol. 37, 01.10.2016, p. 44-52.

Research output: Contribution to journalArticle

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abstract = "A synergistic interaction between gallic acid (GA) and UV-A light (UV-A + GA) to inactivate E. coli O157:H7 in spinach wash water and in biofilm was evaluated. A 30-min exposure to UV-A light in presence of 10 mM GA had relevant biological effect in the inactivation of E. coli O157:H7 in suspension in the absence (> 5 log(CFU/mL)) and the presence of organic content (> 3-log(CFU/mL) in 2000 mg O2/L COD (Chemical Oxygen Demand) organic load), and resulted in ~ 80{\%} decrease in the metabolic activity of E. coli O157:H7 biofilm. GA solutions could be recycled through at least 3-cycles of UV-A treatment without a significant loss in antibacterial effect. Catalase reduced the extent of E. coli O157:H7 inactivation from the UV-A + GA treatment suggesting that generation of hydrogen peroxide was partially responsible for the observed antimicrobial effect. The UV-A + GA treatment was also found to be effective in causing > 3 log(CFU/mL) reductions in E. coli O157:H7 on the surface of spinach leaves. UV-A + GA treatment can serve as an effective intervention in the fresh produce sanitation. Industrial relevance The results of this study show that a synergistic interaction between gallic acid (GA) and UV-A (365 nm) light is an effective treatment for sanitation of fresh produce and water used to wash fresh produce. It was also found to be effective against the E.coli O157:H7 biofilm. The attractive attributes of this technology include a relatively low cost; specific, light-triggered activity; non-toxic nature and scalability. Thus, this technology has potential to replace conventional chemical sanitizer-based sanitation approaches.",
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