Antimicrobial Effect of Photosensitized Rose Bengal on Bacteria and Viruses in Model Wash Water

Andrea Cossu, Duygu Ercan, Rohan V. Tikekar, Nitin Nitin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Peroxides and chlorite-based sanitizers are commonly used for washing procedures. The efficacy of these sanitizers is significantly reduced in the presence of organic content and may result in the formation of harmful secondary products. Therefore, new food-grade sanitizers with enhanced antimicrobial efficacy are needed. In this work, we tested a visible light-activated photosensitizer, Rose Bengal (RB), for the reduction of microbial load in simulated wash water. Escherichia coli BL21 and bacteriophage T7 were selected as model bacterial and viral targets, respectively. Effects of duration of illumination, growth phase of the bacterium, and the presence of organic matter on efficacy of inactivation were evaluated. Photosensitized RB was able to achieve 6 log colony-forming units (CFU)/mL reduction in the exponential-phase bacteria in the presence of high organic content (2000 ppm LB broth) within 45 min of treatment. The results also demonstrated that the stationary-phase microbes were significantly more resistant to photoinactivation as compared to the exponential-phase microbes at both low and high organic loads (200 and 2000 ppm). The results indicate that RB dye can be internalized in bacteria and induces damage to the cell membrane upon photoactivation. Viral inactivation studies demonstrated that photoactivation of RB can achieve 5 log PFU/mL reduction in viral load in the presence of 2000 ppm LB after 30 min of treatment. Overall, these results highlight the efficacy of RB as an antimicrobial against bacteria and viruses in simulated wash water and demonstrate its potential as an alternative sanitizer for the food industry.

Original languageEnglish (US)
Pages (from-to)441-451
Number of pages11
JournalFood and Bioprocess Technology
Volume9
Issue number3
DOIs
StatePublished - Mar 1 2016

Fingerprint

Rose Bengal
anti-infective properties
Viruses
sanitizers
Bacteria
viruses
Water
bacteria
water
inactivation
anti-infective agents
Bacteriophages
Photosensitizers
Virus Inactivation
Bacteriophage T7
microorganisms
chlorites
Cell membranes
Peroxides
Washing

Keywords

  • Escherichia coli
  • Model wash water
  • Photosensitization
  • Rose Bengal
  • T7 bacteriophage

ASJC Scopus subject areas

  • Food Science
  • Process Chemistry and Technology
  • Safety, Risk, Reliability and Quality
  • Industrial and Manufacturing Engineering

Cite this

Antimicrobial Effect of Photosensitized Rose Bengal on Bacteria and Viruses in Model Wash Water. / Cossu, Andrea; Ercan, Duygu; Tikekar, Rohan V.; Nitin, Nitin.

In: Food and Bioprocess Technology, Vol. 9, No. 3, 01.03.2016, p. 441-451.

Research output: Contribution to journalArticle

Cossu, Andrea ; Ercan, Duygu ; Tikekar, Rohan V. ; Nitin, Nitin. / Antimicrobial Effect of Photosensitized Rose Bengal on Bacteria and Viruses in Model Wash Water. In: Food and Bioprocess Technology. 2016 ; Vol. 9, No. 3. pp. 441-451.
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