Antigenically variable Borrelia burgdorferi isolated from cottontail rabbits and Ixodes dentatus in rural and urban areas

J. F. Anderson, L. A. Magnarelli, Rance B Lefebvre, T. G. Andreadis, J. B. McAninch, G. C. Perng, R. C. Johnson

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Spirochetes were isolated from 71 subadult Ixodes dentatus removed from cottontail rabbits captured in Millbrook, N.Y., and in New York, N.Y. Spirochetes were also cultured from kidney tissues of six rabbits. While all isolates reacted with monoclonal antibody H9724, which identifies the spirochetes as borreliae, more than half did not bind with antibody H5332 and even fewer reacted with H3TS, both of which were produced to outer surface protein A of Borrelia burgdorferi. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein profiles of three isolates differed from one another and from all previously characterized B. burgdorferi strains from humans, ticks, and wildlife in North America. The 12 periplasmic flagella that originated subterminally from each pointed end of a rabbit Borrelia isolate contrasted with the 11 or fewer flagella of B. burgdorferi reported previously from North America. Although DNA homology and restriction endonuclease analysis also revealed differences among a rabbit kidney isolate, an I. dentatus isolate, and B. burgdorferi B31, similarities were sufficient to lead us to conclude that the borreliae in rabbits and I. dentatus are B. burgdorferi. Enzyme-linked immunosorbent assay titers of sera from humans with diagnosed Lyme disease to rabbit tick B. burgdorferi were often similar to one another and to those recorded for a reference B. burgdorferi strain.

Original languageEnglish (US)
Pages (from-to)13-20
Number of pages8
JournalJournal of Clinical Microbiology
Volume27
Issue number1
StatePublished - 1989
Externally publishedYes

Fingerprint

Ixodes
Borrelia burgdorferi
Rabbits
Borrelia
Spirochaetales
Flagella
Ticks
North America
Kidney
Lyme Disease
Deoxyribonuclease I
DNA Restriction Enzymes
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Antibodies
Serum

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Anderson, J. F., Magnarelli, L. A., Lefebvre, R. B., Andreadis, T. G., McAninch, J. B., Perng, G. C., & Johnson, R. C. (1989). Antigenically variable Borrelia burgdorferi isolated from cottontail rabbits and Ixodes dentatus in rural and urban areas. Journal of Clinical Microbiology, 27(1), 13-20.

Antigenically variable Borrelia burgdorferi isolated from cottontail rabbits and Ixodes dentatus in rural and urban areas. / Anderson, J. F.; Magnarelli, L. A.; Lefebvre, Rance B; Andreadis, T. G.; McAninch, J. B.; Perng, G. C.; Johnson, R. C.

In: Journal of Clinical Microbiology, Vol. 27, No. 1, 1989, p. 13-20.

Research output: Contribution to journalArticle

Anderson, JF, Magnarelli, LA, Lefebvre, RB, Andreadis, TG, McAninch, JB, Perng, GC & Johnson, RC 1989, 'Antigenically variable Borrelia burgdorferi isolated from cottontail rabbits and Ixodes dentatus in rural and urban areas', Journal of Clinical Microbiology, vol. 27, no. 1, pp. 13-20.
Anderson, J. F. ; Magnarelli, L. A. ; Lefebvre, Rance B ; Andreadis, T. G. ; McAninch, J. B. ; Perng, G. C. ; Johnson, R. C. / Antigenically variable Borrelia burgdorferi isolated from cottontail rabbits and Ixodes dentatus in rural and urban areas. In: Journal of Clinical Microbiology. 1989 ; Vol. 27, No. 1. pp. 13-20.
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abstract = "Spirochetes were isolated from 71 subadult Ixodes dentatus removed from cottontail rabbits captured in Millbrook, N.Y., and in New York, N.Y. Spirochetes were also cultured from kidney tissues of six rabbits. While all isolates reacted with monoclonal antibody H9724, which identifies the spirochetes as borreliae, more than half did not bind with antibody H5332 and even fewer reacted with H3TS, both of which were produced to outer surface protein A of Borrelia burgdorferi. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein profiles of three isolates differed from one another and from all previously characterized B. burgdorferi strains from humans, ticks, and wildlife in North America. The 12 periplasmic flagella that originated subterminally from each pointed end of a rabbit Borrelia isolate contrasted with the 11 or fewer flagella of B. burgdorferi reported previously from North America. Although DNA homology and restriction endonuclease analysis also revealed differences among a rabbit kidney isolate, an I. dentatus isolate, and B. burgdorferi B31, similarities were sufficient to lead us to conclude that the borreliae in rabbits and I. dentatus are B. burgdorferi. Enzyme-linked immunosorbent assay titers of sera from humans with diagnosed Lyme disease to rabbit tick B. burgdorferi were often similar to one another and to those recorded for a reference B. burgdorferi strain.",
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