Antigenic relationships among homologous structural polypeptides of porcine, feline, and canine coronaviruses

M. C. Horzinek, H. Lutz, Niels C Pedersen

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Abstract

Transmissible gastroenteritis virus of swine (TGEV), feline infectious peritonitis virus (FIPV), and canine coronavirus were studied with respect to their serological cross-reactivity in homologous and heterologous virus neutralization, immune precipitation of radiolabeled TGEV, electroblotting, and enzyme-linked immunosorbent assay using individual virion polypeptides prepared by polyacrylamide gel electrophoresis. TGEV was neutralized by feline anti-FIPV serum, and the reaction was potentiated by complement; heterologous neutralization involved antibody reacting with the peplomer protein (P), the envelope protein (E), and cellular (glycolipid) components incorporated into the TGEV membrane. Electrophoretic analysis of immune precipitates containing [35S]methionine-labeled disrupted TGEV and feline anti-FIPV antibody confirmed the reaction with the P and E polypeptides and showed the nucleocapsid protein (N) in addition. Electroblotting, followed by incubation with antibody, 125I-labeled protein A, and fluorography, disclosed cross-reactions between the three viruses at the N and E levels and revealed differences in the apparent molecular weights of the latter. Enzyme immunoassays performed with standard amounts of immobilized P, N, and E polypeptides of the three viruses showed recognition of the antigens by homologous and heterologous antibody to comparable degrees. These results indicate a close antigenic relationship between TGEV, FIPV, and canine coronavirus due to common determinants on the three major virion proteins. The taxonomic implications of these findings are discussed.

Original languageEnglish (US)
Pages (from-to)1148-1155
Number of pages8
JournalInfection and Immunity
Volume37
Issue number3
StatePublished - 1982
Externally publishedYes

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Canine Coronavirus
Feline Coronavirus
Transmissible gastroenteritis virus
Swine
Peptides
Felidae
Viruses
Virion
Antibodies
Photofluorography
Heterophile Antibodies
Nucleocapsid Proteins
Precipitins
Proteins
Glycolipids
Cross Reactions
Staphylococcal Protein A
Immunoenzyme Techniques
Immunoprecipitation
Methionine

ASJC Scopus subject areas

  • Immunology

Cite this

Antigenic relationships among homologous structural polypeptides of porcine, feline, and canine coronaviruses. / Horzinek, M. C.; Lutz, H.; Pedersen, Niels C.

In: Infection and Immunity, Vol. 37, No. 3, 1982, p. 1148-1155.

Research output: Contribution to journalArticle

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