Antigenic diversity of granulocytic Ehrlichia isolates from humans in Wisconsin and New York and a horse in California

Kristin M. Asanovich, Johan S. Bakken, John E. Madigan, Maria Aguero-Rosenfeld, Gary P. Wormser, J. Stephen Dumler

Research output: Contribution to journalArticle

102 Scopus citations

Abstract

The agent of human granulocytic ehrlichiosis (HGE), Ehrlichia phagocytophila, and Ehrlichia equi are very similar. HGE is of variable severity. Genetic and antigenic differences among 3 human isolates (Webster, Spooner, and NY-8) and 1 horse isolate (MRK) were evaluated. The 16S rRNA gene sequences were identical in all human isolates. By use of 5 homologous antisera from these 3 humans and 1 horse and an additional 5 antisera in heterologous reactions, the immunodominant antigens of each isolate were noted to differ in molecular size: 43 kDa in the Webster (Wisconsin) isolate, 46 kDa in the Spooner (Wisconsin) isolate, 42 and 45 kDa in the NY-8 (New York State) isolate, and a 42 kDa doublet in the E. equi MRK isolate from California. Two sera from a Wisconsin patient reacted weakly or not at all with the NY-8 isolate. Antigenic structural diversity exists among otherwise indistinguishable granulocytic ehrlichial isolates.

Original languageEnglish (US)
Pages (from-to)1029-1034
Number of pages6
JournalJournal of Infectious Diseases
Volume176
Issue number4
StatePublished - 1997

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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    Asanovich, K. M., Bakken, J. S., Madigan, J. E., Aguero-Rosenfeld, M., Wormser, G. P., & Dumler, J. S. (1997). Antigenic diversity of granulocytic Ehrlichia isolates from humans in Wisconsin and New York and a horse in California. Journal of Infectious Diseases, 176(4), 1029-1034.