Anticancer effects of tanshinone I in human non-small cell lung cancer

Chen Yu Lee, Hui Fang Sher, Huei Wen Chen, Chun Chi Liu, Ching-Hsien Chen, Choun Sea Lin, Pan Chyr Yang, Hsin Sheng Tsay, Jeremy J.W. Chen

Research output: Contribution to journalArticle

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Abstract

Tanshinones are the major bioactive compounds of Salvia miltiorrhiza Bunge (Danshen) roots, which are used in many therapeutic remedies in Chinese traditional medicine. We investigated the anticancer effects of tanshinones on the highly invasive human lung adenocarcinoma cell line, CL1-5. Tanshinone I significantly inhibited migration, invasion, and gelatinase activity in macrophage-conditioned medium-stimulated CL1-5 cells in vitro and also reduced the tumorigenesis and metastasis in CL1-5-bearing severe combined immunodeficient mice. Unlike tanshinone IIA, which induces cell apoptosis, tanshinone I did not have direct cytotoxicity. Real-time quantitative PCR, luciferase reporter assay, and electrophoretic mobility shift assay revealed that tanshinone I reduces the transcriptional activity of interleukin-8, the angiogenic factor involved in cancer metastasis, by attenuating the DNA-binding activity of activator protein-1 and nuclear factor-κB in conditioned medium-stimulated CL1-5 cells. Microarray and pathway analysis of tumor-related genes identified the differentially expressed genes responding to tanshinone I, which may be associated with the Ras-mitogen-activated protein kinase and Rac1 signaling pathways. These results suggest that tanshinone I exhibits anticancer effects both in vitro and in vivo and that these effects are mediated at least partly through the interleukin-8, Ras-mitogen-activated protein kinase, and Rac1 signaling pathways. Although tanshinone I has a remarkable anticancer action, its potential anticoagulant effect should be noted and evaluated.

Original languageEnglish (US)
Pages (from-to)3527-3538
Number of pages12
JournalMolecular Cancer Therapeutics
Volume7
Issue number11
DOIs
StatePublished - Nov 1 2008
Externally publishedYes

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Non-Small Cell Lung Carcinoma
Salvia miltiorrhiza
Conditioned Culture Medium
Mitogen-Activated Protein Kinases
Interleukin-8
Neoplasm Metastasis
tanshinone
Gelatinases
SCID Mice
Angiogenesis Inducing Agents
Chinese Traditional Medicine
Transcription Factor AP-1
Electrophoretic Mobility Shift Assay
Microarray Analysis
Luciferases
Anticoagulants
Genes
Action Potentials
Real-Time Polymerase Chain Reaction
Neoplasms

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Lee, C. Y., Sher, H. F., Chen, H. W., Liu, C. C., Chen, C-H., Lin, C. S., ... Chen, J. J. W. (2008). Anticancer effects of tanshinone I in human non-small cell lung cancer. Molecular Cancer Therapeutics, 7(11), 3527-3538. https://doi.org/10.1158/1535-7163.MCT-07-2288

Anticancer effects of tanshinone I in human non-small cell lung cancer. / Lee, Chen Yu; Sher, Hui Fang; Chen, Huei Wen; Liu, Chun Chi; Chen, Ching-Hsien; Lin, Choun Sea; Yang, Pan Chyr; Tsay, Hsin Sheng; Chen, Jeremy J.W.

In: Molecular Cancer Therapeutics, Vol. 7, No. 11, 01.11.2008, p. 3527-3538.

Research output: Contribution to journalArticle

Lee, CY, Sher, HF, Chen, HW, Liu, CC, Chen, C-H, Lin, CS, Yang, PC, Tsay, HS & Chen, JJW 2008, 'Anticancer effects of tanshinone I in human non-small cell lung cancer', Molecular Cancer Therapeutics, vol. 7, no. 11, pp. 3527-3538. https://doi.org/10.1158/1535-7163.MCT-07-2288
Lee, Chen Yu ; Sher, Hui Fang ; Chen, Huei Wen ; Liu, Chun Chi ; Chen, Ching-Hsien ; Lin, Choun Sea ; Yang, Pan Chyr ; Tsay, Hsin Sheng ; Chen, Jeremy J.W. / Anticancer effects of tanshinone I in human non-small cell lung cancer. In: Molecular Cancer Therapeutics. 2008 ; Vol. 7, No. 11. pp. 3527-3538.
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