TY - JOUR
T1 - Antibody response of two populations of common carp, Cyprinus carpio L., exposed to koi herpesvirus
AU - St-Hilaire, S.
AU - Beevers, N.
AU - Joiner, C.
AU - Hedrick, Ronald
AU - Way, K.
PY - 2009/4/1
Y1 - 2009/4/1
N2 - Common carp, Cyprinus carpio L., exp7osed to koi herpesvirus (KHV) may become persistently infected and populations containing such virus-infected individuals may transmit the virus to other fish when co-habited. Detection of virus-infected fish in a population is thus critical to surveillance and control programmes for KHV. A study was therefore designed to detect anti-KHV serum antibodies, with an enzyme-linked immunosorbent assay, in common carp following experimental exposures to KHV under varying environmental conditions. The study determined that a proportion of fish within a population experimentally exposed to KHV (at least 10-25%) develop high antibody titres (1/1600 or greater) to the virus, and this immunological response was detectable for several months (observed at the termination of the experiments at 65, 46 and 27 weeks post-exposure). Furthermore, this response was detected in one population of fish that did not succumb to a high level of mortality when maintained at water temperatures that were non-permissive for KHV. Elevating the water temperatures to permissive conditions for KHV resulted in recurrence of disease despite the presence of anti-virus antibodies, suggesting that serum antibodies alone are not protective under the conditions of our trials.
AB - Common carp, Cyprinus carpio L., exp7osed to koi herpesvirus (KHV) may become persistently infected and populations containing such virus-infected individuals may transmit the virus to other fish when co-habited. Detection of virus-infected fish in a population is thus critical to surveillance and control programmes for KHV. A study was therefore designed to detect anti-KHV serum antibodies, with an enzyme-linked immunosorbent assay, in common carp following experimental exposures to KHV under varying environmental conditions. The study determined that a proportion of fish within a population experimentally exposed to KHV (at least 10-25%) develop high antibody titres (1/1600 or greater) to the virus, and this immunological response was detectable for several months (observed at the termination of the experiments at 65, 46 and 27 weeks post-exposure). Furthermore, this response was detected in one population of fish that did not succumb to a high level of mortality when maintained at water temperatures that were non-permissive for KHV. Elevating the water temperatures to permissive conditions for KHV resulted in recurrence of disease despite the presence of anti-virus antibodies, suggesting that serum antibodies alone are not protective under the conditions of our trials.
KW - Cyprinus carpio
KW - Enzyme-linked immunosorbent assay
KW - Koi herpesvirus
KW - Persistent infections
KW - Serology
UR - http://www.scopus.com/inward/record.url?scp=63149102119&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=63149102119&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2761.2008.00993.x
DO - 10.1111/j.1365-2761.2008.00993.x
M3 - Article
C2 - 19236553
AN - SCOPUS:63149102119
VL - 32
SP - 311
EP - 320
JO - Journal of Fish Diseases
JF - Journal of Fish Diseases
SN - 0140-7775
IS - 4
ER -