Abstract
Antibody-based constructs genetically engineered from genes of diverse origin provide a remarkable opportunity to develop functional molecular imaging techniques and specific molecular targeted radionuclide therapies. Phage display libraries of antibody fragment genes can be used to select antibody-based constructs that bind any chosen epitope. A large naive human antibody-based library was used to illustrate binding of antibody constructs to a variety of common and unique antigens. Antibody-based libraries from hybridoma cells, lymphocytes from immunized humans or from mice and human antibody repertoires produced in transgenic mice have also been described. Several orders of magnitude of affinity enhancement can be achieved by random or site specific mutations of the selected binding peptide domains of the scFv. Affinities (k(d)) as high as 10-11 M (10 pM) for affinity-matured scFv have been documented. Such gene libraries thus offer an almost limitless variety of antibody-based molecular binding peptide modules that can be used in creative ways for the construction of new targeting agents for functional or molecular imaging and therapy.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 284-295 |
| Number of pages | 12 |
| Journal | Quarterly Journal of Nuclear Medicine |
| Volume | 44 |
| Issue number | 3 |
| State | Published - 2000 |
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Keywords
- Antibodies bispecific
- Breast neoplasms
- Genetic engineering
- Lymphoma
- Nuclear medicine
- Page display library
- Radioimmunotherapy
- Radionuclide imaging
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging
Cite this
Antibody phage display applications for nuclear medicine imaging and therapy. / Winthrop, M. D.; Denardo, Gerald L; Denardo, S. J.
In: Quarterly Journal of Nuclear Medicine, Vol. 44, No. 3, 2000, p. 284-295.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Antibody phage display applications for nuclear medicine imaging and therapy
AU - Winthrop, M. D.
AU - Denardo, Gerald L
AU - Denardo, S. J.
PY - 2000
Y1 - 2000
N2 - Antibody-based constructs genetically engineered from genes of diverse origin provide a remarkable opportunity to develop functional molecular imaging techniques and specific molecular targeted radionuclide therapies. Phage display libraries of antibody fragment genes can be used to select antibody-based constructs that bind any chosen epitope. A large naive human antibody-based library was used to illustrate binding of antibody constructs to a variety of common and unique antigens. Antibody-based libraries from hybridoma cells, lymphocytes from immunized humans or from mice and human antibody repertoires produced in transgenic mice have also been described. Several orders of magnitude of affinity enhancement can be achieved by random or site specific mutations of the selected binding peptide domains of the scFv. Affinities (k(d)) as high as 10-11 M (10 pM) for affinity-matured scFv have been documented. Such gene libraries thus offer an almost limitless variety of antibody-based molecular binding peptide modules that can be used in creative ways for the construction of new targeting agents for functional or molecular imaging and therapy.
AB - Antibody-based constructs genetically engineered from genes of diverse origin provide a remarkable opportunity to develop functional molecular imaging techniques and specific molecular targeted radionuclide therapies. Phage display libraries of antibody fragment genes can be used to select antibody-based constructs that bind any chosen epitope. A large naive human antibody-based library was used to illustrate binding of antibody constructs to a variety of common and unique antigens. Antibody-based libraries from hybridoma cells, lymphocytes from immunized humans or from mice and human antibody repertoires produced in transgenic mice have also been described. Several orders of magnitude of affinity enhancement can be achieved by random or site specific mutations of the selected binding peptide domains of the scFv. Affinities (k(d)) as high as 10-11 M (10 pM) for affinity-matured scFv have been documented. Such gene libraries thus offer an almost limitless variety of antibody-based molecular binding peptide modules that can be used in creative ways for the construction of new targeting agents for functional or molecular imaging and therapy.
KW - Antibodies bispecific
KW - Breast neoplasms
KW - Genetic engineering
KW - Lymphoma
KW - Nuclear medicine
KW - Page display library
KW - Radioimmunotherapy
KW - Radionuclide imaging
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UR - http://www.scopus.com/inward/citedby.url?scp=0033784713&partnerID=8YFLogxK
M3 - Review article
C2 - 11105591
AN - SCOPUS:0033784713
VL - 44
SP - 284
EP - 295
JO - Quarterly Journal of Nuclear Medicine and Molecular Imaging
JF - Quarterly Journal of Nuclear Medicine and Molecular Imaging
SN - 1824-4785
IS - 3
ER -