Abstract
Antisera raised in rabbits against galactocerebroside bind to bovine oligodendroglia in suspension in significant titer as demonstrated by indirect immunofluorescence. Absorption of antigalactocerebroside antiserum with galactocerebroside, oligodendroglia, or myelin markedly reduces the antigalactocerebroside antibody titer as measured by a radioimmunoprecipitation test as well as the binding to oligodendroglia. Incubation with some other galactose-containing glycolipids results in a parallel decrease in binding to oligodendroglia and reduction in antigalactocerebroside antibody titer. Antigalactocerebroside antibodies provide a useful and specific tool with which to study development of oligodendroglia and myelin as well as immunopathologic mechanisms which might be involved in demyelinating diseases.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 65-73 |
| Number of pages | 9 |
| Journal | Journal of the Neurological Sciences |
| Volume | 40 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1979 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Aging
- Clinical Neurology
- Surgery
- Neuroscience(all)
- Developmental Neuroscience
- Neurology
Cite this
Antibodies to galactocerebroside bind to oligodendroglia in suspension culture. / Lisak, Robert P.; Abramsky, Oded; Dorfman, Susan H.; George, Joan; Manning, Margaret C.; Pleasure, David E; Saida, Takahiko; Silbergerg, Donald H.
In: Journal of the Neurological Sciences, Vol. 40, No. 1, 1979, p. 65-73.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Antibodies to galactocerebroside bind to oligodendroglia in suspension culture
AU - Lisak, Robert P.
AU - Abramsky, Oded
AU - Dorfman, Susan H.
AU - George, Joan
AU - Manning, Margaret C.
AU - Pleasure, David E
AU - Saida, Takahiko
AU - Silbergerg, Donald H.
PY - 1979
Y1 - 1979
N2 - Antisera raised in rabbits against galactocerebroside bind to bovine oligodendroglia in suspension in significant titer as demonstrated by indirect immunofluorescence. Absorption of antigalactocerebroside antiserum with galactocerebroside, oligodendroglia, or myelin markedly reduces the antigalactocerebroside antibody titer as measured by a radioimmunoprecipitation test as well as the binding to oligodendroglia. Incubation with some other galactose-containing glycolipids results in a parallel decrease in binding to oligodendroglia and reduction in antigalactocerebroside antibody titer. Antigalactocerebroside antibodies provide a useful and specific tool with which to study development of oligodendroglia and myelin as well as immunopathologic mechanisms which might be involved in demyelinating diseases.
AB - Antisera raised in rabbits against galactocerebroside bind to bovine oligodendroglia in suspension in significant titer as demonstrated by indirect immunofluorescence. Absorption of antigalactocerebroside antiserum with galactocerebroside, oligodendroglia, or myelin markedly reduces the antigalactocerebroside antibody titer as measured by a radioimmunoprecipitation test as well as the binding to oligodendroglia. Incubation with some other galactose-containing glycolipids results in a parallel decrease in binding to oligodendroglia and reduction in antigalactocerebroside antibody titer. Antigalactocerebroside antibodies provide a useful and specific tool with which to study development of oligodendroglia and myelin as well as immunopathologic mechanisms which might be involved in demyelinating diseases.
UR - http://www.scopus.com/inward/record.url?scp=0018326893&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018326893&partnerID=8YFLogxK
U2 - 10.1016/0022-510X(79)90009-1
DO - 10.1016/0022-510X(79)90009-1
M3 - Article
AN - SCOPUS:0018326893
VL - 40
SP - 65
EP - 73
JO - Journal of the Neurological Sciences
JF - Journal of the Neurological Sciences
SN - 0022-510X
IS - 1
ER -