Antibodies to galactocerebroside bind to oligodendroglia in suspension culture

Robert P. Lisak, Oded Abramsky, Susan H. Dorfman, Joan George, Margaret C. Manning, David E Pleasure, Takahiko Saida, Donald H. Silbergerg

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Antisera raised in rabbits against galactocerebroside bind to bovine oligodendroglia in suspension in significant titer as demonstrated by indirect immunofluorescence. Absorption of antigalactocerebroside antiserum with galactocerebroside, oligodendroglia, or myelin markedly reduces the antigalactocerebroside antibody titer as measured by a radioimmunoprecipitation test as well as the binding to oligodendroglia. Incubation with some other galactose-containing glycolipids results in a parallel decrease in binding to oligodendroglia and reduction in antigalactocerebroside antibody titer. Antigalactocerebroside antibodies provide a useful and specific tool with which to study development of oligodendroglia and myelin as well as immunopathologic mechanisms which might be involved in demyelinating diseases.

Original languageEnglish (US)
Pages (from-to)65-73
Number of pages9
JournalJournal of the Neurological Sciences
Volume40
Issue number1
DOIs
StatePublished - 1979
Externally publishedYes

ASJC Scopus subject areas

  • Aging
  • Clinical Neurology
  • Surgery
  • Neuroscience(all)
  • Developmental Neuroscience
  • Neurology

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    Lisak, R. P., Abramsky, O., Dorfman, S. H., George, J., Manning, M. C., Pleasure, D. E., Saida, T., & Silbergerg, D. H. (1979). Antibodies to galactocerebroside bind to oligodendroglia in suspension culture. Journal of the Neurological Sciences, 40(1), 65-73. https://doi.org/10.1016/0022-510X(79)90009-1