Antibodies to galactocerebroside bind to oligodendroglia in suspension culture

Robert P. Lisak; Oded Abramsky; Susan H. Dorfman; Joan George; Margaret C. Manning; David E Pleasure; Takahiko Saida; Donald H. Silbergerg

doi:10.1016/0022-510X(79)90009-1

Antibodies to galactocerebroside bind to oligodendroglia in suspension culture

Robert P. Lisak, Oded Abramsky, Susan H. Dorfman, Joan George, Margaret C. Manning, David E Pleasure, Takahiko Saida, Donald H. Silbergerg

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Antisera raised in rabbits against galactocerebroside bind to bovine oligodendroglia in suspension in significant titer as demonstrated by indirect immunofluorescence. Absorption of antigalactocerebroside antiserum with galactocerebroside, oligodendroglia, or myelin markedly reduces the antigalactocerebroside antibody titer as measured by a radioimmunoprecipitation test as well as the binding to oligodendroglia. Incubation with some other galactose-containing glycolipids results in a parallel decrease in binding to oligodendroglia and reduction in antigalactocerebroside antibody titer. Antigalactocerebroside antibodies provide a useful and specific tool with which to study development of oligodendroglia and myelin as well as immunopathologic mechanisms which might be involved in demyelinating diseases.

Original language	English (US)
Pages (from-to)	65-73
Number of pages	9
Journal	Journal of the Neurological Sciences
Volume	40
Issue number	1
DOIs	https://doi.org/10.1016/0022-510X(79)90009-1
State	Published - 1979
Externally published	Yes

ASJC Scopus subject areas

Aging
Clinical Neurology
Surgery
Neuroscience(all)
Developmental Neuroscience
Neurology

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Antibodies to galactocerebroside bind to oligodendroglia in suspension culture. / Lisak, Robert P.; Abramsky, Oded; Dorfman, Susan H.; George, Joan; Manning, Margaret C.; Pleasure, David E; Saida, Takahiko; Silbergerg, Donald H.

In: Journal of the Neurological Sciences, Vol. 40, No. 1, 1979, p. 65-73.

Research output: Contribution to journal › Article › peer-review

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abstract = "Antisera raised in rabbits against galactocerebroside bind to bovine oligodendroglia in suspension in significant titer as demonstrated by indirect immunofluorescence. Absorption of antigalactocerebroside antiserum with galactocerebroside, oligodendroglia, or myelin markedly reduces the antigalactocerebroside antibody titer as measured by a radioimmunoprecipitation test as well as the binding to oligodendroglia. Incubation with some other galactose-containing glycolipids results in a parallel decrease in binding to oligodendroglia and reduction in antigalactocerebroside antibody titer. Antigalactocerebroside antibodies provide a useful and specific tool with which to study development of oligodendroglia and myelin as well as immunopathologic mechanisms which might be involved in demyelinating diseases.",

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AU - Lisak, Robert P.

AU - Abramsky, Oded

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AU - George, Joan

AU - Manning, Margaret C.

AU - Pleasure, David E

AU - Saida, Takahiko

AU - Silbergerg, Donald H.

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AB - Antisera raised in rabbits against galactocerebroside bind to bovine oligodendroglia in suspension in significant titer as demonstrated by indirect immunofluorescence. Absorption of antigalactocerebroside antiserum with galactocerebroside, oligodendroglia, or myelin markedly reduces the antigalactocerebroside antibody titer as measured by a radioimmunoprecipitation test as well as the binding to oligodendroglia. Incubation with some other galactose-containing glycolipids results in a parallel decrease in binding to oligodendroglia and reduction in antigalactocerebroside antibody titer. Antigalactocerebroside antibodies provide a useful and specific tool with which to study development of oligodendroglia and myelin as well as immunopathologic mechanisms which might be involved in demyelinating diseases.

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