Abstract
Reasons for performing study: Early diagnosis and monitoring progression of chronic diseases in elastin-rich tissues, such as chronic progressive lymphoedema in draught horses and chronic obstructive pulmonary disease (COPD) is still a real challenge in the horse. Use of an enzyme-linked immunosorbent assay (ELISA) to detect anti-elastin antibody (AEAb) levels might be useful to assess the status of such diseases. Baseline levels, representing physiological breakdown of elastin in normal horses, are not available at present. Hypothesis: Levels of AEAb in healthy horses are generally low and follow the same age-related pattern as found in man. Therefore, elevation of AEAb levels in serum can be used to evaluate pathological elastin breakdown in elastin-rich tissues. Methods: Sera of 84 clinically healthy Warmblood horses were evaluated for the presence of AEAbs by means of a modified version of an ELISA technique used in man. The horses were divided in 5 age groups: A) <4 months; B) 4-23 months; C) 2-3 years; D) 4-10 years; and E) >11 years. Results: Antibodies to elastin were found in all equine serum samples tested. Their levels were lowest in Group A, low in Groups B and E and highest in animals age 2-10 years. Conclusions: Measuring AEAbs in serum of horses by an ELISA technique proved to be possible and levels were stable during well-defined life stages. Potential relevance: Changes in AEAb levels are expected to be useful for early diagnosis and for monitoring progression of diseases that affect elastin-rich tissues, such as chronic progressive lymphoedema and COPD.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 414-416 |
| Number of pages | 3 |
| Journal | Equine Veterinary Journal |
| Volume | 39 |
| Issue number | 5 |
| DOIs | |
| State | Published - Sep 2007 |
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Keywords
- Anti-elastin antibodies
- Elastin
- ELISA
- Horse
ASJC Scopus subject areas
- veterinary(all)
Cite this
Antibodies to elastin peptides in sera of Warmblood horses at different ages. / Van Brantegem, L.; De Cock, H. E V; Affolter, Verena K; Duchateau, L.; Govaere, J.; Ferraro, G. L.; Ducatelle, R.
In: Equine Veterinary Journal, Vol. 39, No. 5, 09.2007, p. 414-416.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Antibodies to elastin peptides in sera of Warmblood horses at different ages
AU - Van Brantegem, L.
AU - De Cock, H. E V
AU - Affolter, Verena K
AU - Duchateau, L.
AU - Govaere, J.
AU - Ferraro, G. L.
AU - Ducatelle, R.
PY - 2007/9
Y1 - 2007/9
N2 - Reasons for performing study: Early diagnosis and monitoring progression of chronic diseases in elastin-rich tissues, such as chronic progressive lymphoedema in draught horses and chronic obstructive pulmonary disease (COPD) is still a real challenge in the horse. Use of an enzyme-linked immunosorbent assay (ELISA) to detect anti-elastin antibody (AEAb) levels might be useful to assess the status of such diseases. Baseline levels, representing physiological breakdown of elastin in normal horses, are not available at present. Hypothesis: Levels of AEAb in healthy horses are generally low and follow the same age-related pattern as found in man. Therefore, elevation of AEAb levels in serum can be used to evaluate pathological elastin breakdown in elastin-rich tissues. Methods: Sera of 84 clinically healthy Warmblood horses were evaluated for the presence of AEAbs by means of a modified version of an ELISA technique used in man. The horses were divided in 5 age groups: A) <4 months; B) 4-23 months; C) 2-3 years; D) 4-10 years; and E) >11 years. Results: Antibodies to elastin were found in all equine serum samples tested. Their levels were lowest in Group A, low in Groups B and E and highest in animals age 2-10 years. Conclusions: Measuring AEAbs in serum of horses by an ELISA technique proved to be possible and levels were stable during well-defined life stages. Potential relevance: Changes in AEAb levels are expected to be useful for early diagnosis and for monitoring progression of diseases that affect elastin-rich tissues, such as chronic progressive lymphoedema and COPD.
AB - Reasons for performing study: Early diagnosis and monitoring progression of chronic diseases in elastin-rich tissues, such as chronic progressive lymphoedema in draught horses and chronic obstructive pulmonary disease (COPD) is still a real challenge in the horse. Use of an enzyme-linked immunosorbent assay (ELISA) to detect anti-elastin antibody (AEAb) levels might be useful to assess the status of such diseases. Baseline levels, representing physiological breakdown of elastin in normal horses, are not available at present. Hypothesis: Levels of AEAb in healthy horses are generally low and follow the same age-related pattern as found in man. Therefore, elevation of AEAb levels in serum can be used to evaluate pathological elastin breakdown in elastin-rich tissues. Methods: Sera of 84 clinically healthy Warmblood horses were evaluated for the presence of AEAbs by means of a modified version of an ELISA technique used in man. The horses were divided in 5 age groups: A) <4 months; B) 4-23 months; C) 2-3 years; D) 4-10 years; and E) >11 years. Results: Antibodies to elastin were found in all equine serum samples tested. Their levels were lowest in Group A, low in Groups B and E and highest in animals age 2-10 years. Conclusions: Measuring AEAbs in serum of horses by an ELISA technique proved to be possible and levels were stable during well-defined life stages. Potential relevance: Changes in AEAb levels are expected to be useful for early diagnosis and for monitoring progression of diseases that affect elastin-rich tissues, such as chronic progressive lymphoedema and COPD.
KW - Anti-elastin antibodies
KW - Elastin
KW - ELISA
KW - Horse
UR - http://www.scopus.com/inward/record.url?scp=38449120182&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38449120182&partnerID=8YFLogxK
U2 - 10.2746/042516407X204573
DO - 10.2746/042516407X204573
M3 - Article
C2 - 17910265
AN - SCOPUS:38449120182
VL - 39
SP - 414
EP - 416
JO - Equine veterinary journal. Supplement
JF - Equine veterinary journal. Supplement
SN - 2042-3306
IS - 5
ER -