Anti-ulcer efficacy of soluble epoxide hydrolase inhibitor TPPU on diclofenac-induced intestinal ulcers

Sumanta Kumar Goswami, Debin Wan, Jun Yang, Carlos A. Trindade Da Silva, Christophe Morisseau, Sean D. Kodani, Guang Yu Yang, Bora Inceoglu, Bruce D. Hammock

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23 Scopus citations

Abstract

Proton pump inhibitors such as omeprazole (OME) reduce the severity of gastrointestinal (GI) ulcers induced by nonsteroidal anti-inflammatory drugs (NSAIDs) but can also increase the chance of dysbiosis. The aim of this study was to test the hypothesis that preventive use of a soluble epoxide hydrolase inhibitor (sEHI) such as TPPU can decrease NSAID-induced ulcers by increasing anti-inflammatory epoxyeicosatrienoic acids (EETs). Dose- [10, 30, and 100 mg/kg, by mouth (PO)] and time-dependent (6 and 18 hours) ulcerative effects of diclofenac sodium (DCF, an NSAID) were studied in the small intestine of Swiss Webster mice. Dose-dependent effects of TPPU (0.001-0.1 mg/kg per day for 7 days, in drinking water) were evaluated in DCF-induced intestinal toxicity and compared with OME (20 mg/kg, PO). In addition, the effect of treatment was studied on levels of Hb in blood, EETs in plasma, inflammatory markers such as myeloperoxidase (MPO) in intestinal tissue homogenates, and tissue necrosis factor-α (TNF-α) in serum. DCF dose dependently induced ulcers that were associated with both a significant (P < 0.05) loss of Hb and an increase in the level of MPO and TNF-α, with severity of ulceration highest at 18 hours. Pretreatment with TPPU dose dependently prevented ulcer formation by DCF, increased the levels of epoxy fatty acids, including EETs, and TPPU's efficacy was comparable to OME. TPPU significantly (P < 0.05) reversed the effect of DCF on the level of Hb, MPO, and TNF-α. Thus sEHI might be useful in the management of NSAID-induced ulcers.

Original languageEnglish (US)
Pages (from-to)529-536
Number of pages8
JournalJournal of Pharmacology and Experimental Therapeutics
Volume357
Issue number3
DOIs
StatePublished - Jun 1 2016

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ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Goswami, S. K., Wan, D., Yang, J., Trindade Da Silva, C. A., Morisseau, C., Kodani, S. D., Yang, G. Y., Inceoglu, B., & Hammock, B. D. (2016). Anti-ulcer efficacy of soluble epoxide hydrolase inhibitor TPPU on diclofenac-induced intestinal ulcers. Journal of Pharmacology and Experimental Therapeutics, 357(3), 529-536. https://doi.org/10.1124/jpet.116.232108