Anti-insect toxin 5 (AaIT5) from Androctonus australis

Yoshiaki Nakagawa, Young Moo Lee, Elisabeth Lehmberg, Rafael Herrmann, Revital Herrmann, Haim Moskowitz, A. Daniel Jones, Bruce D. Hammock

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

An insect-selective scorpion toxin (AaIT5) was purified from the venom of the North African scorpion Androctonus australis, and its amino acid sequence was determined by a combination of automated Edman degradation, electrospray-ionization mass spectrometry, and sequence alignment. This insect toxin is very potent against the tobacco budworm, Heliothis virescens (100% lethal dose <1.8 μg/100 mg body mass) and shows a distinct insect specificity and various symptoms. It is not toxic to mice after subcutaneous injection. The molecular mass of this toxin is 6882 Da and the amino acid sequence is similar to those of Androctonus australis anti-insect toxin 4 (AaIT4), Leiuris quinquestriatus depressant anti-insect toxins (LqhIT2, LqqIT2), and Buthotus judaicus depressant anti-insect toxin (BjIT2).

Original languageEnglish (US)
Pages (from-to)496-501
Number of pages6
JournalEuropean Journal of Biochemistry
Volume246
Issue number2
StatePublished - 1997

Fingerprint

Insects
Amino Acids
Electrospray ionization
Tobacco
Poisons
Venoms
Molecular mass
Mass spectrometry
Amino Acid Sequence
Degradation
Scorpions
Electrospray Ionization Mass Spectrometry
Sequence Alignment
Subcutaneous Injections
4-(3'-di(2-chloroethyl)aminopropionyl)biphenyl
scorpion toxin AaIT5
LqhIT2 insect toxin

Keywords

  • Amino acid sequence
  • Androctonus australis
  • Anti-insect toxin
  • Neurotoxin
  • Scorpion toxin

ASJC Scopus subject areas

  • Biochemistry

Cite this

Nakagawa, Y., Lee, Y. M., Lehmberg, E., Herrmann, R., Herrmann, R., Moskowitz, H., ... Hammock, B. D. (1997). Anti-insect toxin 5 (AaIT5) from Androctonus australis. European Journal of Biochemistry, 246(2), 496-501.

Anti-insect toxin 5 (AaIT5) from Androctonus australis. / Nakagawa, Yoshiaki; Lee, Young Moo; Lehmberg, Elisabeth; Herrmann, Rafael; Herrmann, Revital; Moskowitz, Haim; Daniel Jones, A.; Hammock, Bruce D.

In: European Journal of Biochemistry, Vol. 246, No. 2, 1997, p. 496-501.

Research output: Contribution to journalArticle

Nakagawa, Y, Lee, YM, Lehmberg, E, Herrmann, R, Herrmann, R, Moskowitz, H, Daniel Jones, A & Hammock, BD 1997, 'Anti-insect toxin 5 (AaIT5) from Androctonus australis', European Journal of Biochemistry, vol. 246, no. 2, pp. 496-501.
Nakagawa Y, Lee YM, Lehmberg E, Herrmann R, Herrmann R, Moskowitz H et al. Anti-insect toxin 5 (AaIT5) from Androctonus australis. European Journal of Biochemistry. 1997;246(2):496-501.
Nakagawa, Yoshiaki ; Lee, Young Moo ; Lehmberg, Elisabeth ; Herrmann, Rafael ; Herrmann, Revital ; Moskowitz, Haim ; Daniel Jones, A. ; Hammock, Bruce D. / Anti-insect toxin 5 (AaIT5) from Androctonus australis. In: European Journal of Biochemistry. 1997 ; Vol. 246, No. 2. pp. 496-501.
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