Anti-insect toxin 5 (AaIT5) from Androctonus australis

Yoshiaki Nakagawa; Young Moo Lee; Elisabeth Lehmberg; Rafael Herrmann; Revital Herrmann; Haim Moskowitz; A. Daniel Jones; Bruce D. Hammock

Anti-insect toxin 5 (AaIT5) from Androctonus australis

Yoshiaki Nakagawa, Young Moo Lee, Elisabeth Lehmberg, Rafael Herrmann, Revital Herrmann, Haim Moskowitz, A. Daniel Jones, Bruce D. Hammock

Entomology

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

An insect-selective scorpion toxin (AaIT5) was purified from the venom of the North African scorpion Androctonus australis, and its amino acid sequence was determined by a combination of automated Edman degradation, electrospray-ionization mass spectrometry, and sequence alignment. This insect toxin is very potent against the tobacco budworm, Heliothis virescens (100% lethal dose <1.8 μg/100 mg body mass) and shows a distinct insect specificity and various symptoms. It is not toxic to mice after subcutaneous injection. The molecular mass of this toxin is 6882 Da and the amino acid sequence is similar to those of Androctonus australis anti-insect toxin 4 (AaIT4), Leiuris quinquestriatus depressant anti-insect toxins (LqhIT2, LqqIT2), and Buthotus judaicus depressant anti-insect toxin (BjIT2).

Original language	English (US)
Pages (from-to)	496-501
Number of pages	6
Journal	European Journal of Biochemistry
Volume	246
Issue number	2
State	Published - 1997

Keywords

Amino acid sequence
Androctonus australis
Anti-insect toxin
Neurotoxin
Scorpion toxin

ASJC Scopus subject areas

Biochemistry

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title = "Anti-insect toxin 5 (AaIT5) from Androctonus australis",

abstract = "An insect-selective scorpion toxin (AaIT5) was purified from the venom of the North African scorpion Androctonus australis, and its amino acid sequence was determined by a combination of automated Edman degradation, electrospray-ionization mass spectrometry, and sequence alignment. This insect toxin is very potent against the tobacco budworm, Heliothis virescens (100% lethal dose <1.8 μg/100 mg body mass) and shows a distinct insect specificity and various symptoms. It is not toxic to mice after subcutaneous injection. The molecular mass of this toxin is 6882 Da and the amino acid sequence is similar to those of Androctonus australis anti-insect toxin 4 (AaIT4), Leiuris quinquestriatus depressant anti-insect toxins (LqhIT2, LqqIT2), and Buthotus judaicus depressant anti-insect toxin (BjIT2).",

keywords = "Amino acid sequence, Androctonus australis, Anti-insect toxin, Neurotoxin, Scorpion toxin",

author = "Yoshiaki Nakagawa and Lee, {Young Moo} and Elisabeth Lehmberg and Rafael Herrmann and Revital Herrmann and Haim Moskowitz and {Daniel Jones}, A. and Hammock, {Bruce D.}",

year = "1997",

language = "English (US)",

volume = "246",

pages = "496--501",

journal = "FEBS Journal",

issn = "1742-464X",

publisher = "Wiley-Blackwell",

number = "2",

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TY - JOUR

T1 - Anti-insect toxin 5 (AaIT5) from Androctonus australis

AU - Nakagawa, Yoshiaki

AU - Lee, Young Moo

AU - Lehmberg, Elisabeth

AU - Herrmann, Rafael

AU - Herrmann, Revital

AU - Moskowitz, Haim

AU - Daniel Jones, A.

AU - Hammock, Bruce D.

PY - 1997

Y1 - 1997

N2 - An insect-selective scorpion toxin (AaIT5) was purified from the venom of the North African scorpion Androctonus australis, and its amino acid sequence was determined by a combination of automated Edman degradation, electrospray-ionization mass spectrometry, and sequence alignment. This insect toxin is very potent against the tobacco budworm, Heliothis virescens (100% lethal dose <1.8 μg/100 mg body mass) and shows a distinct insect specificity and various symptoms. It is not toxic to mice after subcutaneous injection. The molecular mass of this toxin is 6882 Da and the amino acid sequence is similar to those of Androctonus australis anti-insect toxin 4 (AaIT4), Leiuris quinquestriatus depressant anti-insect toxins (LqhIT2, LqqIT2), and Buthotus judaicus depressant anti-insect toxin (BjIT2).

AB - An insect-selective scorpion toxin (AaIT5) was purified from the venom of the North African scorpion Androctonus australis, and its amino acid sequence was determined by a combination of automated Edman degradation, electrospray-ionization mass spectrometry, and sequence alignment. This insect toxin is very potent against the tobacco budworm, Heliothis virescens (100% lethal dose <1.8 μg/100 mg body mass) and shows a distinct insect specificity and various symptoms. It is not toxic to mice after subcutaneous injection. The molecular mass of this toxin is 6882 Da and the amino acid sequence is similar to those of Androctonus australis anti-insect toxin 4 (AaIT4), Leiuris quinquestriatus depressant anti-insect toxins (LqhIT2, LqqIT2), and Buthotus judaicus depressant anti-insect toxin (BjIT2).

KW - Amino acid sequence

KW - Androctonus australis

KW - Anti-insect toxin

KW - Neurotoxin

KW - Scorpion toxin

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