Abstract
An insect-selective scorpion toxin (AaIT5) was purified from the venom of the North African scorpion Androctonus australis, and its amino acid sequence was determined by a combination of automated Edman degradation, electrospray-ionization mass spectrometry, and sequence alignment. This insect toxin is very potent against the tobacco budworm, Heliothis virescens (100% lethal dose <1.8 μg/100 mg body mass) and shows a distinct insect specificity and various symptoms. It is not toxic to mice after subcutaneous injection. The molecular mass of this toxin is 6882 Da and the amino acid sequence is similar to those of Androctonus australis anti-insect toxin 4 (AaIT4), Leiuris quinquestriatus depressant anti-insect toxins (LqhIT2, LqqIT2), and Buthotus judaicus depressant anti-insect toxin (BjIT2).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 496-501 |
| Number of pages | 6 |
| Journal | European Journal of Biochemistry |
| Volume | 246 |
| Issue number | 2 |
| State | Published - 1997 |
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Keywords
- Amino acid sequence
- Androctonus australis
- Anti-insect toxin
- Neurotoxin
- Scorpion toxin
ASJC Scopus subject areas
- Biochemistry
Cite this
Anti-insect toxin 5 (AaIT5) from Androctonus australis. / Nakagawa, Yoshiaki; Lee, Young Moo; Lehmberg, Elisabeth; Herrmann, Rafael; Herrmann, Revital; Moskowitz, Haim; Daniel Jones, A.; Hammock, Bruce D.
In: European Journal of Biochemistry, Vol. 246, No. 2, 1997, p. 496-501.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Anti-insect toxin 5 (AaIT5) from Androctonus australis
AU - Nakagawa, Yoshiaki
AU - Lee, Young Moo
AU - Lehmberg, Elisabeth
AU - Herrmann, Rafael
AU - Herrmann, Revital
AU - Moskowitz, Haim
AU - Daniel Jones, A.
AU - Hammock, Bruce D.
PY - 1997
Y1 - 1997
N2 - An insect-selective scorpion toxin (AaIT5) was purified from the venom of the North African scorpion Androctonus australis, and its amino acid sequence was determined by a combination of automated Edman degradation, electrospray-ionization mass spectrometry, and sequence alignment. This insect toxin is very potent against the tobacco budworm, Heliothis virescens (100% lethal dose <1.8 μg/100 mg body mass) and shows a distinct insect specificity and various symptoms. It is not toxic to mice after subcutaneous injection. The molecular mass of this toxin is 6882 Da and the amino acid sequence is similar to those of Androctonus australis anti-insect toxin 4 (AaIT4), Leiuris quinquestriatus depressant anti-insect toxins (LqhIT2, LqqIT2), and Buthotus judaicus depressant anti-insect toxin (BjIT2).
AB - An insect-selective scorpion toxin (AaIT5) was purified from the venom of the North African scorpion Androctonus australis, and its amino acid sequence was determined by a combination of automated Edman degradation, electrospray-ionization mass spectrometry, and sequence alignment. This insect toxin is very potent against the tobacco budworm, Heliothis virescens (100% lethal dose <1.8 μg/100 mg body mass) and shows a distinct insect specificity and various symptoms. It is not toxic to mice after subcutaneous injection. The molecular mass of this toxin is 6882 Da and the amino acid sequence is similar to those of Androctonus australis anti-insect toxin 4 (AaIT4), Leiuris quinquestriatus depressant anti-insect toxins (LqhIT2, LqqIT2), and Buthotus judaicus depressant anti-insect toxin (BjIT2).
KW - Amino acid sequence
KW - Androctonus australis
KW - Anti-insect toxin
KW - Neurotoxin
KW - Scorpion toxin
UR - http://www.scopus.com/inward/record.url?scp=0030910076&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030910076&partnerID=8YFLogxK
M3 - Article
VL - 246
SP - 496
EP - 501
JO - FEBS Journal
JF - FEBS Journal
SN - 1742-464X
IS - 2
ER -