Abstract
An insect-selective scorpion toxin (AaIT5) was purified from the venom of the North African scorpion Androctonus australis, and its amino acid sequence was determined by a combination of automated Edman degradation, electrospray-ionization mass spectrometry, and sequence alignment. This insect toxin is very potent against the tobacco budworm, Heliothis virescens (100% lethal dose <1.8 μg/100 mg body mass) and shows a distinct insect specificity and various symptoms. It is not toxic to mice after subcutaneous injection. The molecular mass of this toxin is 6882 Da and the amino acid sequence is similar to those of Androctonus australis anti-insect toxin 4 (AaIT4), Leiuris quinquestriatus depressant anti-insect toxins (LqhIT2, LqqIT2), and Buthotus judaicus depressant anti-insect toxin (BjIT2).
Original language | English (US) |
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Pages (from-to) | 496-501 |
Number of pages | 6 |
Journal | European Journal of Biochemistry |
Volume | 246 |
Issue number | 2 |
State | Published - 1997 |
Keywords
- Amino acid sequence
- Androctonus australis
- Anti-insect toxin
- Neurotoxin
- Scorpion toxin
ASJC Scopus subject areas
- Biochemistry