Anti-HLA class I antibody inhibits Staphylococcus enterotoxin A (SEA)-induced proliferation of human PBMC

Padmavathi Baskar, James Hildreth, Francis J. Chrest, James E. Nagel, William H. Adler

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The antigen-presenting role of major histocompatibility complex (MHC) Class I molecules in the activation of appropriately restricted T cells is well documented. Now growing evidence indicates that MHC Class I molecules can, in addition, exert a regulatory effect and influence the resulting immune responses. In this report, we show that a monoclonal antibody (mAb) directed against a conserved region of the human leukocyte antigens (HLA)-A, -B, and -C was able to inhibit proliferation of human peripheral blood mononuclear cells induced by the superantigen, Staphylococcus enterotoxin A. While anti-HLA inhibition was associated with a decrease in IL-2 receptor (IL-2R) expression, the addition of exogenous IL-2 did not restore the proliferative response in the presence of anti-HLA mAb. The inhibition of DNA synthesis was also associated with a decrease in the expression of the early activation marker CD69. These results suggest a critical role for HLA Class I molecules in the early events of human lymphocyte activation and proliferation as well as in their expression of the IL-2R.

Original languageEnglish (US)
Pages (from-to)135-138
Number of pages4
JournalCellular Immunology
Volume172
Issue number1
DOIs
StatePublished - Aug 25 1996
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Fingerprint Dive into the research topics of 'Anti-HLA class I antibody inhibits Staphylococcus enterotoxin A (SEA)-induced proliferation of human PBMC'. Together they form a unique fingerprint.

Cite this