Anti-Galectin-3 IgG autoantibodies in patients with Crohn's disease characterized by means of phage display peptide libraries

Erika Jensen-Jarolim, Christian Neumann, Georg Oberhuber, Regina Gscheidlinger, Csilla Neuchrist, Walter Reinisch, Riaz I. Zuberi, Edward Penner, Fu-Tong Liu, George Boltz-Nitulescu

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Galectin-3, a member of β-galactoside-binding lectins, is expressed and secreted by a variety of cell types including human intestinal epithelial cells. The presence of anti-galectin-3 antibody in the sera of patients was analyzed by immunoblotting using recombinant human galectin-3. A substantially higher percentage of sera from Crohn's disease patients contained anti-galectin-3 IgG autoantibodies than from patients with ulcerative colitis, primary biliary cirrhosis, or autoimmune hepatitis and of apparently healthy control volunteers. In Crohn's disease patients the titer of autoantibodies was high and interestingly correlated negatively with disease activity. To characterize and generate artificial epitopes (mimotopes), the anti-galectin-3 monoclonal antibodies A3A12 and B2C10 were used for biopannings of phage display nonapeptide libraries. These mimotopes interfered with the binding of autoantibodies to recombinant and native intestinal epithelial galectin-3. Our data may suggest that galectin-3 mimotopes could be used for the induction of IgG with desired specificity to regulate immune responses in Crohn's disease patients.

Original languageEnglish (US)
Pages (from-to)348-356
Number of pages9
JournalJournal of Clinical Immunology
Volume21
Issue number5
DOIs
StatePublished - 2001

Keywords

  • Autoantibody
  • Crohn's disease
  • Galectin-3
  • Inflammatory bowel disease
  • Phage display

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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  • Cite this

    Jensen-Jarolim, E., Neumann, C., Oberhuber, G., Gscheidlinger, R., Neuchrist, C., Reinisch, W., Zuberi, R. I., Penner, E., Liu, F-T., & Boltz-Nitulescu, G. (2001). Anti-Galectin-3 IgG autoantibodies in patients with Crohn's disease characterized by means of phage display peptide libraries. Journal of Clinical Immunology, 21(5), 348-356. https://doi.org/10.1023/A:1012240719801