Abstract
Galectin-3, a member of β-galactoside-binding lectins, is expressed and secreted by a variety of cell types including human intestinal epithelial cells. The presence of anti-galectin-3 antibody in the sera of patients was analyzed by immunoblotting using recombinant human galectin-3. A substantially higher percentage of sera from Crohn's disease patients contained anti-galectin-3 IgG autoantibodies than from patients with ulcerative colitis, primary biliary cirrhosis, or autoimmune hepatitis and of apparently healthy control volunteers. In Crohn's disease patients the titer of autoantibodies was high and interestingly correlated negatively with disease activity. To characterize and generate artificial epitopes (mimotopes), the anti-galectin-3 monoclonal antibodies A3A12 and B2C10 were used for biopannings of phage display nonapeptide libraries. These mimotopes interfered with the binding of autoantibodies to recombinant and native intestinal epithelial galectin-3. Our data may suggest that galectin-3 mimotopes could be used for the induction of IgG with desired specificity to regulate immune responses in Crohn's disease patients.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 348-356 |
| Number of pages | 9 |
| Journal | Journal of Clinical Immunology |
| Volume | 21 |
| Issue number | 5 |
| DOIs | |
| State | Published - 2001 |
Fingerprint
Keywords
- Autoantibody
- Crohn's disease
- Galectin-3
- Inflammatory bowel disease
- Phage display
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
Anti-Galectin-3 IgG autoantibodies in patients with Crohn's disease characterized by means of phage display peptide libraries. / Jensen-Jarolim, Erika; Neumann, Christian; Oberhuber, Georg; Gscheidlinger, Regina; Neuchrist, Csilla; Reinisch, Walter; Zuberi, Riaz I.; Penner, Edward; Liu, Fu-Tong; Boltz-Nitulescu, George.
In: Journal of Clinical Immunology, Vol. 21, No. 5, 2001, p. 348-356.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Anti-Galectin-3 IgG autoantibodies in patients with Crohn's disease characterized by means of phage display peptide libraries
AU - Jensen-Jarolim, Erika
AU - Neumann, Christian
AU - Oberhuber, Georg
AU - Gscheidlinger, Regina
AU - Neuchrist, Csilla
AU - Reinisch, Walter
AU - Zuberi, Riaz I.
AU - Penner, Edward
AU - Liu, Fu-Tong
AU - Boltz-Nitulescu, George
PY - 2001
Y1 - 2001
N2 - Galectin-3, a member of β-galactoside-binding lectins, is expressed and secreted by a variety of cell types including human intestinal epithelial cells. The presence of anti-galectin-3 antibody in the sera of patients was analyzed by immunoblotting using recombinant human galectin-3. A substantially higher percentage of sera from Crohn's disease patients contained anti-galectin-3 IgG autoantibodies than from patients with ulcerative colitis, primary biliary cirrhosis, or autoimmune hepatitis and of apparently healthy control volunteers. In Crohn's disease patients the titer of autoantibodies was high and interestingly correlated negatively with disease activity. To characterize and generate artificial epitopes (mimotopes), the anti-galectin-3 monoclonal antibodies A3A12 and B2C10 were used for biopannings of phage display nonapeptide libraries. These mimotopes interfered with the binding of autoantibodies to recombinant and native intestinal epithelial galectin-3. Our data may suggest that galectin-3 mimotopes could be used for the induction of IgG with desired specificity to regulate immune responses in Crohn's disease patients.
AB - Galectin-3, a member of β-galactoside-binding lectins, is expressed and secreted by a variety of cell types including human intestinal epithelial cells. The presence of anti-galectin-3 antibody in the sera of patients was analyzed by immunoblotting using recombinant human galectin-3. A substantially higher percentage of sera from Crohn's disease patients contained anti-galectin-3 IgG autoantibodies than from patients with ulcerative colitis, primary biliary cirrhosis, or autoimmune hepatitis and of apparently healthy control volunteers. In Crohn's disease patients the titer of autoantibodies was high and interestingly correlated negatively with disease activity. To characterize and generate artificial epitopes (mimotopes), the anti-galectin-3 monoclonal antibodies A3A12 and B2C10 were used for biopannings of phage display nonapeptide libraries. These mimotopes interfered with the binding of autoantibodies to recombinant and native intestinal epithelial galectin-3. Our data may suggest that galectin-3 mimotopes could be used for the induction of IgG with desired specificity to regulate immune responses in Crohn's disease patients.
KW - Autoantibody
KW - Crohn's disease
KW - Galectin-3
KW - Inflammatory bowel disease
KW - Phage display
UR - http://www.scopus.com/inward/record.url?scp=0034759454&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034759454&partnerID=8YFLogxK
U2 - 10.1023/A:1012240719801
DO - 10.1023/A:1012240719801
M3 - Article
C2 - 11720007
AN - SCOPUS:0034759454
VL - 21
SP - 348
EP - 356
JO - Journal of Clinical Immunology
JF - Journal of Clinical Immunology
SN - 0271-9142
IS - 5
ER -