Anthocyanidins inhibit activator protein 1 activity and cell transformation: Structure-activity relationship and molecular mechanisms

De Xing Hou, Keiko Kai, Jian-Jian Li, Shigang Lin, Norihiko Terahara, Mika Wakamatsu, Makoto Fujii, Mattew R. Young, Nancy Colburn

Research output: Contribution to journalArticle

168 Citations (Scopus)

Abstract

Anthocyanins are the chemical components that give the intense color to many fruits and vegetables, such as blueberries, red cabbages and purple sweet potatoes. Extensive studies have indicated that anthocyanins have strong antioxidant activities. To investigate the mechanism of anthocyanidins as an anticancer food source, six kinds of anthocyanidins representing the aglycons of most anthocyanins, were used to examine their effects on tumor promotion in mouse JB6 cells, a validated model for screening cancer chemopreventive agents and elucidating the molecular mechanisms. Of the six anthocyanins tested, only those with an ortho-dihydroxyphenyl structure on the B-ring suppressed 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell transformation and activator protein-1 transactivation, suggesting that the ortho-dihydroxyphenyl may contribute to the inhibitory action. Delphinidin, but not peonidin, blocked the phosphorylation of protein kinases in the extracellular signal-regulated protein kinase (ERK) pathway at early times and the c-Jun N-terminal kinase (JNK) signaling pathway at later times. p38 kinase was not inhibited by delphinidin. Furthermore, two mitogen-activated protein kinase (MAPK) specific inhibitors (SP600125 for JNK and UO126 for ERK) could specifically block the activation of JNK and ERK and cell transformation. Those results demonstrate that anthocyanidins contribute to the inhibition of tumorigenesis by blocking activation of the MAPK pathway. These findings provide the first molecular basis for the anticarcinogenic action of anthocyanidins.

Original languageEnglish (US)
Pages (from-to)29-36
Number of pages8
JournalCarcinogenesis
Volume25
Issue number1
DOIs
StatePublished - Jan 2004
Externally publishedYes

Fingerprint

Anthocyanins
Transcription Factor AP-1
Structure-Activity Relationship
delphinidin
JNK Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinases
Protein Kinases
Blueberry Plants
Ipomoea batatas
MAP Kinase Signaling System
Brassica
Extracellular Signal-Regulated MAP Kinases
Tetradecanoylphorbol Acetate
Protein Kinase Inhibitors
Early Detection of Cancer
Vegetables
Transcriptional Activation
Fruit
Carcinogenesis
Phosphotransferases

ASJC Scopus subject areas

  • Cancer Research

Cite this

Anthocyanidins inhibit activator protein 1 activity and cell transformation : Structure-activity relationship and molecular mechanisms. / Hou, De Xing; Kai, Keiko; Li, Jian-Jian; Lin, Shigang; Terahara, Norihiko; Wakamatsu, Mika; Fujii, Makoto; Young, Mattew R.; Colburn, Nancy.

In: Carcinogenesis, Vol. 25, No. 1, 01.2004, p. 29-36.

Research output: Contribution to journalArticle

Hou, De Xing ; Kai, Keiko ; Li, Jian-Jian ; Lin, Shigang ; Terahara, Norihiko ; Wakamatsu, Mika ; Fujii, Makoto ; Young, Mattew R. ; Colburn, Nancy. / Anthocyanidins inhibit activator protein 1 activity and cell transformation : Structure-activity relationship and molecular mechanisms. In: Carcinogenesis. 2004 ; Vol. 25, No. 1. pp. 29-36.
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