Antenatal steroid and tracheal occlusion restore vascular endothelial growth factor receptors in congenital diaphragmatic hernia rat model

Augusto F. Schmidt, Frances L L Gonçalves, Azize C C Nassr, Luis A V D Pereira, Diana L Farmer, Lourenço Sbragia

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objective: Investigate the effects of antenatal steroids and tracheal occlusion on pulmonary expression of vascular endothelial growth factor receptors in rats with nitrofen-induced congenital diaphragmatic hernia. Study Design: Fetuses were exposed to nitrofen at embryonic day 9.5. Subgroups received dexamethasone or were operated on for tracheal occlusion, or received combined treatment. Morphologic variables were recorded. To analyze vascular endothelial growth factor receptor 1 and vascular endothelial growth factor receptor 2 expression, we performed Western blotting and immunohistochemistry. Morphologic variables were analyzed by analysis of variance and immunohistochemistry by Kruskal-Wallis test. Results: Congenital diaphragmatic hernia decreased body weight, total lung weight, and lung-to-body weight ratio. Tracheal occlusion increased total lung weight and lung-to-body weight ratio (P < .05). Fetuses with congenital diaphragmatic hernia had reduced vascular endothelial growth factor receptor 1 and vascular endothelial growth factor receptor 2 expression, whereas steroids and tracheal occlusion increased their expression. Combined treatment increased expression of receptors, but had no additive effect. Conclusion: Vascular endothelial growth factor signaling disruption may be associated with pulmonary hypertension in congenital diaphragmatic hernia. Tracheal occlusion and steroids provide a pathway for restoring expression of vascular endothelial growth factor receptors.

Original languageEnglish (US)
JournalAmerican Journal of Obstetrics and Gynecology
Volume203
Issue number2
DOIs
StatePublished - 2010

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Vascular Endothelial Growth Factor Receptor
Steroids
Lung
Vascular Endothelial Growth Factor Receptor-1
Vascular Endothelial Growth Factor Receptor-2
Body Weight
Fetus
Immunohistochemistry
Weights and Measures
Pulmonary Hypertension
Dexamethasone
Vascular Endothelial Growth Factor A
Analysis of Variance
Western Blotting
Congenital Diaphragmatic Hernias
Therapeutics

Keywords

  • congenital diaphragmatic hernia
  • rat model
  • steroids
  • tracheal occlusion
  • vascular endothelial growth factor receptor

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Antenatal steroid and tracheal occlusion restore vascular endothelial growth factor receptors in congenital diaphragmatic hernia rat model. / Schmidt, Augusto F.; Gonçalves, Frances L L; Nassr, Azize C C; Pereira, Luis A V D; Farmer, Diana L; Sbragia, Lourenço.

In: American Journal of Obstetrics and Gynecology, Vol. 203, No. 2, 2010.

Research output: Contribution to journalArticle

Schmidt, Augusto F. ; Gonçalves, Frances L L ; Nassr, Azize C C ; Pereira, Luis A V D ; Farmer, Diana L ; Sbragia, Lourenço. / Antenatal steroid and tracheal occlusion restore vascular endothelial growth factor receptors in congenital diaphragmatic hernia rat model. In: American Journal of Obstetrics and Gynecology. 2010 ; Vol. 203, No. 2.
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