Anopheles darlingi polytene chromosomes: Revised maps including newly described inversions and evidence for population structure in Manaus

Anthony J. Cornel, Katherine K. Brisco, Wanderli P. Tadei, Nágila F C Secundino, Miriam S. Rafael, Allan K R Galardo, Jansen F. Medeiros, Felipe A C Pessoa, Claudia M. Ríos-Velásquez, Yoosook Lee, Paulo F P Pimenta, Gregory C Lanzaro

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Salivary gland polytene chromosomes of 4th instar Anopheles darlingi Root were examined from multiple locations in the Brazilian Amazon. Minor modifications were made to existing polytene photomaps. These included changes to the breakpoint positions of several previously described paracentric inversions and descriptions of four new paracentric inversions, two on the right arm of chromosome 3 and two on the left arm of chromosome 3 that were found in multiple locations. A total of 18 inversions on the X (n = 1) chromosome, chromosome 2 (n = 7) and 3 (n = 11) were scored for 83 individuals from Manaus, Macapá and Porto Velho municipalities. The frequency of 2Ra inversion karyotypes in Manaus shows significant deficiency of heterozygotes (p < 0.0009). No significant linkage disequilibrium was found between inversions on chromosome 2 and 3. We hypothesize that at least two sympatric subpopulations exist within the An. darlingi population at Manaus based on inversion frequencies.

Original languageEnglish (US)
Pages (from-to)335-346
Number of pages12
JournalMemorias do Instituto Oswaldo Cruz
Volume111
Issue number5
DOIs
StatePublished - May 1 2016

Keywords

  • Anopheles darlingi
  • Chromosome inversions
  • Salivary gland polytene chromosomes

ASJC Scopus subject areas

  • Microbiology (medical)

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    Cornel, A. J., Brisco, K. K., Tadei, W. P., Secundino, N. F. C., Rafael, M. S., Galardo, A. K. R., Medeiros, J. F., Pessoa, F. A. C., Ríos-Velásquez, C. M., Lee, Y., Pimenta, P. F. P., & Lanzaro, G. C. (2016). Anopheles darlingi polytene chromosomes: Revised maps including newly described inversions and evidence for population structure in Manaus. Memorias do Instituto Oswaldo Cruz, 111(5), 335-346. https://doi.org/10.1590/0074-02760150470