Animal models for studies on cold-induced platelet activation in human beings

Fern Tablin, Naomi J. Walker, Susan D. Klein, Cara L. Field, John H. Crowe

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

When human platelets are chilled below about 20°C, they spontaneously activate, a phenomenon that limits their storage lifetime. We have previously shown that this activation in chilled human platelets is associated with passage through a lipid phase transition. Because animal models are necessary for investigating methods for cold storage of platelets, it is essential to determine whether such phase transitions and chilling-induced activation are found in these models. In this study we examined platelets from some commonly used animal models - pigs, rhesus monkeys, mice, dogs, and rabbits. Using Fourier transform infrared spectroscopy (FTIR), we detected the thermotropic membrane phase transition in intact platelets and assessed the morphologic response of the platelets to chilling. Statistical analysis of both FTIR and shape change show that of the animal models tested, pig platelets are most similar to human platelets. These studies suggest that pigs and pig platelets are the models of choice for the study of cold-induced platelet activation in human beings.

Original languageEnglish (US)
Pages (from-to)339-346
Number of pages8
JournalJournal of Laboratory and Clinical Medicine
Volume135
Issue number4
StatePublished - 2000

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'Animal models for studies on cold-induced platelet activation in human beings'. Together they form a unique fingerprint.

Cite this