Angiogenesis in high- and low-metastatic mammary tumors an intravital microscopic study

A. T.W. Cheung, P. C.Y. Chen, L. T.J. Young, A. Ndoye, C. Y. Chao, S. Wiltse, W. J. Muller, Robert Cardiff

Research output: Contribution to journalArticle

Abstract

Transplantable mammary tumor lines have been established in nude mice. The high-metastatic (Met-1) line gave rise to detectable pulmonary metastasis (80%; n=39) and differed significantly (P<0.001) from the low-metastatic (Db-7) line (18%; n=22) Computer-assisted intravital microscopy was used to objectively study intratumoral angiogenesis, vascular morphometry, flow and tortuosity characteristics. The microvascular development of the two lines differed in organization (branching, pattern, distribution), however, vessel/venual diameter (range=65-110 (im) and flow velocity (range=0.2-2.7 mm/s) in both lines were measured to be nearly identical Significant intratumoral vessel tortuosity existed inside Met-1, and the tumor vessel tortuosity index (shortest distance between vessel ends/actual vessel length) of Met-1 (range=0.25-0.47; mean=0.39) differed significantly (P<0.001) from Db-7 (range=0.56-0.92, mean=0.81 ). The intratumoral tortuosity and microvascular patterns of Met-1 and Db-7 have been compared with angiogenic microvessel density (MVD) measured in tx-actin stained sections of the tumors Tortuosity and microvascular patterns are unique intratumoral features and can be used to explain the MVD hoi spots identified for human breast cancer prognosis.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996

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Microvessels
Tumors
Breast Neoplasms
Nude Mice
Blood Vessels
Actins
Neoplasms
Neoplasm Metastasis
Flow velocity
Lung
Intravital Microscopy

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Cheung, A. T. W., Chen, P. C. Y., Young, L. T. J., Ndoye, A., Chao, C. Y., Wiltse, S., ... Cardiff, R. (1996). Angiogenesis in high- and low-metastatic mammary tumors an intravital microscopic study. FASEB Journal, 10(3).

Angiogenesis in high- and low-metastatic mammary tumors an intravital microscopic study. / Cheung, A. T.W.; Chen, P. C.Y.; Young, L. T.J.; Ndoye, A.; Chao, C. Y.; Wiltse, S.; Muller, W. J.; Cardiff, Robert.

In: FASEB Journal, Vol. 10, No. 3, 01.12.1996.

Research output: Contribution to journalArticle

Cheung, ATW, Chen, PCY, Young, LTJ, Ndoye, A, Chao, CY, Wiltse, S, Muller, WJ & Cardiff, R 1996, 'Angiogenesis in high- and low-metastatic mammary tumors an intravital microscopic study', FASEB Journal, vol. 10, no. 3.
Cheung ATW, Chen PCY, Young LTJ, Ndoye A, Chao CY, Wiltse S et al. Angiogenesis in high- and low-metastatic mammary tumors an intravital microscopic study. FASEB Journal. 1996 Dec 1;10(3).
Cheung, A. T.W. ; Chen, P. C.Y. ; Young, L. T.J. ; Ndoye, A. ; Chao, C. Y. ; Wiltse, S. ; Muller, W. J. ; Cardiff, Robert. / Angiogenesis in high- and low-metastatic mammary tumors an intravital microscopic study. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
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