Angiogenesis and the expression of vascular endothelial growth factors A and C in squamous cell carcinoma of the piriform fossa

Jarrod J. Homer, Michael G. Prentice, Lynn Cawkwell, Martin Birchall, John Greenman, Nicholas D. Stafford

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Angiogenesis is essential for tumor growth and invasion. Vascular endothelial growth factor A (VEGF-A) is a prime mediator of tumor angiogenesis; VEGF-C, another member of the closely related VEGF family of proteins, has major effects on lymphatic endothelial cells and may be important in the process of lymphatic metastasis. Objectives: To evaluate the expression of these cytokines in hypopharyngeal squamous cell carcinoma and to ascertain the effects of these proteins on lymphatic metastasis and vascular angiogenesis. Design: Retrospective analysis of microvessel density and the expression of VEGF-A and VEGF-C. Setting: An academic referral center. Subjects: Thirty-four patients with stage T2 to T4 squamous cell carcinoma of the piriform fossa. Interventions: Expression of VEGF-A and VEGF-C was determined by immunohistochemistry on formalin-fixed, paraffin-embedded biopsy specimens. Angiogenesis was measured as microvessel density by staining endothelial cells for platelet-endothelial cell adhesion molecule 1/CD31. Results: Of the 34 tumors, 21 had clinicoradiologic evidence of lymphatic metastasis. Expression of VEGF-C was associated with lymphatic metastasis (P<.001), but not with microvessel density. The VEGF-A expression correlated with microvessel density (P<.001), but neither VEGF-A expression nor microvessel density was associated with lymphatic metastasis. Conclusions: The expression of VEGF-C is associated with lymphatic metastasis in squamous cell carcinoma of the piriform fossa. This is not secondary to effects on vascular angiogenesis and is hypothesized to be due to effects on lymphatic endothelial cells.

Original languageEnglish (US)
Pages (from-to)1110-1114
Number of pages5
JournalArchives of Otolaryngology - Head and Neck Surgery
Volume129
Issue number10
DOIs
StatePublished - Oct 2003
Externally publishedYes

Fingerprint

Pyriform Sinus
Vascular Endothelial Growth Factor C
Lymphatic Metastasis
Vascular Endothelial Growth Factor A
Squamous Cell Carcinoma
Microvessels
Endothelial Cells
Blood Vessels
CD31 Antigens
CD4-Positive T-Lymphocytes
Neoplasms
Paraffin
Formaldehyde
Proteins
Referral and Consultation
Immunohistochemistry
Staining and Labeling
Cytokines
Biopsy
Growth

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Angiogenesis and the expression of vascular endothelial growth factors A and C in squamous cell carcinoma of the piriform fossa. / Homer, Jarrod J.; Prentice, Michael G.; Cawkwell, Lynn; Birchall, Martin; Greenman, John; Stafford, Nicholas D.

In: Archives of Otolaryngology - Head and Neck Surgery, Vol. 129, No. 10, 10.2003, p. 1110-1114.

Research output: Contribution to journalArticle

Homer, Jarrod J. ; Prentice, Michael G. ; Cawkwell, Lynn ; Birchall, Martin ; Greenman, John ; Stafford, Nicholas D. / Angiogenesis and the expression of vascular endothelial growth factors A and C in squamous cell carcinoma of the piriform fossa. In: Archives of Otolaryngology - Head and Neck Surgery. 2003 ; Vol. 129, No. 10. pp. 1110-1114.
@article{54dd37b69da141c3b9172f5ea0bc6a1b,
title = "Angiogenesis and the expression of vascular endothelial growth factors A and C in squamous cell carcinoma of the piriform fossa",
abstract = "Background: Angiogenesis is essential for tumor growth and invasion. Vascular endothelial growth factor A (VEGF-A) is a prime mediator of tumor angiogenesis; VEGF-C, another member of the closely related VEGF family of proteins, has major effects on lymphatic endothelial cells and may be important in the process of lymphatic metastasis. Objectives: To evaluate the expression of these cytokines in hypopharyngeal squamous cell carcinoma and to ascertain the effects of these proteins on lymphatic metastasis and vascular angiogenesis. Design: Retrospective analysis of microvessel density and the expression of VEGF-A and VEGF-C. Setting: An academic referral center. Subjects: Thirty-four patients with stage T2 to T4 squamous cell carcinoma of the piriform fossa. Interventions: Expression of VEGF-A and VEGF-C was determined by immunohistochemistry on formalin-fixed, paraffin-embedded biopsy specimens. Angiogenesis was measured as microvessel density by staining endothelial cells for platelet-endothelial cell adhesion molecule 1/CD31. Results: Of the 34 tumors, 21 had clinicoradiologic evidence of lymphatic metastasis. Expression of VEGF-C was associated with lymphatic metastasis (P<.001), but not with microvessel density. The VEGF-A expression correlated with microvessel density (P<.001), but neither VEGF-A expression nor microvessel density was associated with lymphatic metastasis. Conclusions: The expression of VEGF-C is associated with lymphatic metastasis in squamous cell carcinoma of the piriform fossa. This is not secondary to effects on vascular angiogenesis and is hypothesized to be due to effects on lymphatic endothelial cells.",
author = "Homer, {Jarrod J.} and Prentice, {Michael G.} and Lynn Cawkwell and Martin Birchall and John Greenman and Stafford, {Nicholas D.}",
year = "2003",
month = "10",
doi = "10.1001/archotol.129.10.1110",
language = "English (US)",
volume = "129",
pages = "1110--1114",
journal = "JAMA Otolaryngology - Head and Neck Surgery",
issn = "2168-6181",
publisher = "American Medical Association",
number = "10",

}

TY - JOUR

T1 - Angiogenesis and the expression of vascular endothelial growth factors A and C in squamous cell carcinoma of the piriform fossa

AU - Homer, Jarrod J.

AU - Prentice, Michael G.

AU - Cawkwell, Lynn

AU - Birchall, Martin

AU - Greenman, John

AU - Stafford, Nicholas D.

PY - 2003/10

Y1 - 2003/10

N2 - Background: Angiogenesis is essential for tumor growth and invasion. Vascular endothelial growth factor A (VEGF-A) is a prime mediator of tumor angiogenesis; VEGF-C, another member of the closely related VEGF family of proteins, has major effects on lymphatic endothelial cells and may be important in the process of lymphatic metastasis. Objectives: To evaluate the expression of these cytokines in hypopharyngeal squamous cell carcinoma and to ascertain the effects of these proteins on lymphatic metastasis and vascular angiogenesis. Design: Retrospective analysis of microvessel density and the expression of VEGF-A and VEGF-C. Setting: An academic referral center. Subjects: Thirty-four patients with stage T2 to T4 squamous cell carcinoma of the piriform fossa. Interventions: Expression of VEGF-A and VEGF-C was determined by immunohistochemistry on formalin-fixed, paraffin-embedded biopsy specimens. Angiogenesis was measured as microvessel density by staining endothelial cells for platelet-endothelial cell adhesion molecule 1/CD31. Results: Of the 34 tumors, 21 had clinicoradiologic evidence of lymphatic metastasis. Expression of VEGF-C was associated with lymphatic metastasis (P<.001), but not with microvessel density. The VEGF-A expression correlated with microvessel density (P<.001), but neither VEGF-A expression nor microvessel density was associated with lymphatic metastasis. Conclusions: The expression of VEGF-C is associated with lymphatic metastasis in squamous cell carcinoma of the piriform fossa. This is not secondary to effects on vascular angiogenesis and is hypothesized to be due to effects on lymphatic endothelial cells.

AB - Background: Angiogenesis is essential for tumor growth and invasion. Vascular endothelial growth factor A (VEGF-A) is a prime mediator of tumor angiogenesis; VEGF-C, another member of the closely related VEGF family of proteins, has major effects on lymphatic endothelial cells and may be important in the process of lymphatic metastasis. Objectives: To evaluate the expression of these cytokines in hypopharyngeal squamous cell carcinoma and to ascertain the effects of these proteins on lymphatic metastasis and vascular angiogenesis. Design: Retrospective analysis of microvessel density and the expression of VEGF-A and VEGF-C. Setting: An academic referral center. Subjects: Thirty-four patients with stage T2 to T4 squamous cell carcinoma of the piriform fossa. Interventions: Expression of VEGF-A and VEGF-C was determined by immunohistochemistry on formalin-fixed, paraffin-embedded biopsy specimens. Angiogenesis was measured as microvessel density by staining endothelial cells for platelet-endothelial cell adhesion molecule 1/CD31. Results: Of the 34 tumors, 21 had clinicoradiologic evidence of lymphatic metastasis. Expression of VEGF-C was associated with lymphatic metastasis (P<.001), but not with microvessel density. The VEGF-A expression correlated with microvessel density (P<.001), but neither VEGF-A expression nor microvessel density was associated with lymphatic metastasis. Conclusions: The expression of VEGF-C is associated with lymphatic metastasis in squamous cell carcinoma of the piriform fossa. This is not secondary to effects on vascular angiogenesis and is hypothesized to be due to effects on lymphatic endothelial cells.

UR - http://www.scopus.com/inward/record.url?scp=0141891902&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141891902&partnerID=8YFLogxK

U2 - 10.1001/archotol.129.10.1110

DO - 10.1001/archotol.129.10.1110

M3 - Article

C2 - 14568798

AN - SCOPUS:0141891902

VL - 129

SP - 1110

EP - 1114

JO - JAMA Otolaryngology - Head and Neck Surgery

JF - JAMA Otolaryngology - Head and Neck Surgery

SN - 2168-6181

IS - 10

ER -