Abstract
Wound healing is a complex process that requires the intervention of cytoactive factors. The one-time application of soluble factors to a wound bed does not maintain a steady, sufficient concentration. Here we investigated the benefits of anchoring a factor in a wound bed via a tether to endogenous collagen. We used a collagen-mimetic peptide (CMP) as a pylon. The CMP binds to damaged but not intact collagen and thus localizes a pendant cytoactive factor in the regions of a wound bed that require intervention. As a model factor, we chose substance P, a peptide of the tachykinin family that promotes wound healing. Using splinted wounds in db/db mice, we found that the one-time application of a CMP-substance P conjugate enhances wound healing compared to unconjugated substance P and other controls. Specifically, all 16 wounds treated with the conjugate closed more thoroughly and, did so with extensive re-epithelialization and mitigated inflammatory activity. These data validate a simple and general strategy for re-engineering wound beds by the integration of beneficial cytoactive factors.
| Original language | English (US) |
|---|---|
| Journal | Journal of Tissue Engineering and Regenerative Medicine |
| DOIs | |
| State | Accepted/In press - 2014 |
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Keywords
- Collagen
- Extracellular matrix
- Mus musculus
- Peptide
- Substance P
- Synthetic biology
ASJC Scopus subject areas
- Biomaterials
- Medicine (miscellaneous)
- Biomedical Engineering
Cite this
Anchoring a cytoactive factor in a wound bed promotes healing. / Chattopadhyay, Sayani; Guthrie, Kathleen M.; Teixeira, Leandro; Murphy, Christopher J; Dubielzig, Richard R.; Mcanulty, Jonathan F.; Raines, Ronald T.
In: Journal of Tissue Engineering and Regenerative Medicine, 2014.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Anchoring a cytoactive factor in a wound bed promotes healing
AU - Chattopadhyay, Sayani
AU - Guthrie, Kathleen M.
AU - Teixeira, Leandro
AU - Murphy, Christopher J
AU - Dubielzig, Richard R.
AU - Mcanulty, Jonathan F.
AU - Raines, Ronald T.
PY - 2014
Y1 - 2014
N2 - Wound healing is a complex process that requires the intervention of cytoactive factors. The one-time application of soluble factors to a wound bed does not maintain a steady, sufficient concentration. Here we investigated the benefits of anchoring a factor in a wound bed via a tether to endogenous collagen. We used a collagen-mimetic peptide (CMP) as a pylon. The CMP binds to damaged but not intact collagen and thus localizes a pendant cytoactive factor in the regions of a wound bed that require intervention. As a model factor, we chose substance P, a peptide of the tachykinin family that promotes wound healing. Using splinted wounds in db/db mice, we found that the one-time application of a CMP-substance P conjugate enhances wound healing compared to unconjugated substance P and other controls. Specifically, all 16 wounds treated with the conjugate closed more thoroughly and, did so with extensive re-epithelialization and mitigated inflammatory activity. These data validate a simple and general strategy for re-engineering wound beds by the integration of beneficial cytoactive factors.
AB - Wound healing is a complex process that requires the intervention of cytoactive factors. The one-time application of soluble factors to a wound bed does not maintain a steady, sufficient concentration. Here we investigated the benefits of anchoring a factor in a wound bed via a tether to endogenous collagen. We used a collagen-mimetic peptide (CMP) as a pylon. The CMP binds to damaged but not intact collagen and thus localizes a pendant cytoactive factor in the regions of a wound bed that require intervention. As a model factor, we chose substance P, a peptide of the tachykinin family that promotes wound healing. Using splinted wounds in db/db mice, we found that the one-time application of a CMP-substance P conjugate enhances wound healing compared to unconjugated substance P and other controls. Specifically, all 16 wounds treated with the conjugate closed more thoroughly and, did so with extensive re-epithelialization and mitigated inflammatory activity. These data validate a simple and general strategy for re-engineering wound beds by the integration of beneficial cytoactive factors.
KW - Collagen
KW - Extracellular matrix
KW - Mus musculus
KW - Peptide
KW - Substance P
KW - Synthetic biology
UR - http://www.scopus.com/inward/record.url?scp=84896691946&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84896691946&partnerID=8YFLogxK
U2 - 10.1002/term.1886
DO - 10.1002/term.1886
M3 - Article
JO - Journal of Tissue Engineering and Regenerative Medicine
JF - Journal of Tissue Engineering and Regenerative Medicine
SN - 1932-6254
ER -