Anatomy of herpes simplex virus DNA. IX. Apparent exclusion of some parental DNA arrangements in the generation of intertypic (HSV-1 x HSV-2) recombinants

Lawrence S Morse, T. G. Buchman, B. Roizman, P. A. Schaffer

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

We are reporting the physical location of parental DNA sequences in 28 recombinants produced by crossing herpes simplex viruses (HSV) 1 and 2. The parental crosses were of two kinds. In the first, temperature-sensitive mutants of HSV-1 and HSV-2 were crossed to produce wild-type recombinants. In the second, temperature-sensitive mutants of HSV-1 rendered resistant to phosphonoacetic acid were crossed with wild-type HSV-2, and recombinants that multiplied at nonpermissive temperature and were resistant to the drug were selected. The DNAs of the recombinants were mapped with XbaI, EcoRI, HpaI, HsuI, BglII, and, in some instances, KpnI restriction endonucleases. The results were as follows. We established the colinear arrangements of HSV-1 and HSV-2 DNAs. There was extensive interchange of genomic regions, ranging from the exchange of the entire L of S component of HSV DNA to substitutions of regions within the same component. In some recombinants, the reiterated sequences ab and ac bracketing the L and S components of HSV DNA were heterotypic. Most recombinants grew well and showed no obvious defects. The number of crossover events ranged from one to as many as six. Although crossover events occurred throughout the DNA, some clustering of crossover events was observed. Analysis of recombinants permitted localization of several markers used in this study and appears to be a useful technique for marker mapping. As previously reported, HSV DNA consists of four populations, differing in relative orientation of the L and S components. All recombinants could be displayed in one arrangement of L and S such that the number of crossover events was minimized. The data are consistent with the hypothesis that only one arrangement of the parental DNA participates in the generation of recombinants.

Original languageEnglish (US)
Pages (from-to)231-248
Number of pages18
JournalJournal of Virology
Volume24
Issue number1
StatePublished - 1977
Externally publishedYes

Fingerprint

Human herpesvirus 2
Human herpesvirus 1
herpes simplex
DNA viruses
Human Herpesvirus 2
Human Herpesvirus 1
Simplexvirus
Anatomy
DNA
mutants
temperature
recombinant DNA
Temperature
restriction endonucleases
Phosphonoacetic Acid
Recombinant DNA
DNA Restriction Enzymes
genomics
nucleotide sequences
drugs

ASJC Scopus subject areas

  • Immunology

Cite this

Anatomy of herpes simplex virus DNA. IX. Apparent exclusion of some parental DNA arrangements in the generation of intertypic (HSV-1 x HSV-2) recombinants. / Morse, Lawrence S; Buchman, T. G.; Roizman, B.; Schaffer, P. A.

In: Journal of Virology, Vol. 24, No. 1, 1977, p. 231-248.

Research output: Contribution to journalArticle

@article{731b62f80eeb40fea8ab0463d432374f,
title = "Anatomy of herpes simplex virus DNA. IX. Apparent exclusion of some parental DNA arrangements in the generation of intertypic (HSV-1 x HSV-2) recombinants",
abstract = "We are reporting the physical location of parental DNA sequences in 28 recombinants produced by crossing herpes simplex viruses (HSV) 1 and 2. The parental crosses were of two kinds. In the first, temperature-sensitive mutants of HSV-1 and HSV-2 were crossed to produce wild-type recombinants. In the second, temperature-sensitive mutants of HSV-1 rendered resistant to phosphonoacetic acid were crossed with wild-type HSV-2, and recombinants that multiplied at nonpermissive temperature and were resistant to the drug were selected. The DNAs of the recombinants were mapped with XbaI, EcoRI, HpaI, HsuI, BglII, and, in some instances, KpnI restriction endonucleases. The results were as follows. We established the colinear arrangements of HSV-1 and HSV-2 DNAs. There was extensive interchange of genomic regions, ranging from the exchange of the entire L of S component of HSV DNA to substitutions of regions within the same component. In some recombinants, the reiterated sequences ab and ac bracketing the L and S components of HSV DNA were heterotypic. Most recombinants grew well and showed no obvious defects. The number of crossover events ranged from one to as many as six. Although crossover events occurred throughout the DNA, some clustering of crossover events was observed. Analysis of recombinants permitted localization of several markers used in this study and appears to be a useful technique for marker mapping. As previously reported, HSV DNA consists of four populations, differing in relative orientation of the L and S components. All recombinants could be displayed in one arrangement of L and S such that the number of crossover events was minimized. The data are consistent with the hypothesis that only one arrangement of the parental DNA participates in the generation of recombinants.",
author = "Morse, {Lawrence S} and Buchman, {T. G.} and B. Roizman and Schaffer, {P. A.}",
year = "1977",
language = "English (US)",
volume = "24",
pages = "231--248",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Anatomy of herpes simplex virus DNA. IX. Apparent exclusion of some parental DNA arrangements in the generation of intertypic (HSV-1 x HSV-2) recombinants

AU - Morse, Lawrence S

AU - Buchman, T. G.

AU - Roizman, B.

AU - Schaffer, P. A.

PY - 1977

Y1 - 1977

N2 - We are reporting the physical location of parental DNA sequences in 28 recombinants produced by crossing herpes simplex viruses (HSV) 1 and 2. The parental crosses were of two kinds. In the first, temperature-sensitive mutants of HSV-1 and HSV-2 were crossed to produce wild-type recombinants. In the second, temperature-sensitive mutants of HSV-1 rendered resistant to phosphonoacetic acid were crossed with wild-type HSV-2, and recombinants that multiplied at nonpermissive temperature and were resistant to the drug were selected. The DNAs of the recombinants were mapped with XbaI, EcoRI, HpaI, HsuI, BglII, and, in some instances, KpnI restriction endonucleases. The results were as follows. We established the colinear arrangements of HSV-1 and HSV-2 DNAs. There was extensive interchange of genomic regions, ranging from the exchange of the entire L of S component of HSV DNA to substitutions of regions within the same component. In some recombinants, the reiterated sequences ab and ac bracketing the L and S components of HSV DNA were heterotypic. Most recombinants grew well and showed no obvious defects. The number of crossover events ranged from one to as many as six. Although crossover events occurred throughout the DNA, some clustering of crossover events was observed. Analysis of recombinants permitted localization of several markers used in this study and appears to be a useful technique for marker mapping. As previously reported, HSV DNA consists of four populations, differing in relative orientation of the L and S components. All recombinants could be displayed in one arrangement of L and S such that the number of crossover events was minimized. The data are consistent with the hypothesis that only one arrangement of the parental DNA participates in the generation of recombinants.

AB - We are reporting the physical location of parental DNA sequences in 28 recombinants produced by crossing herpes simplex viruses (HSV) 1 and 2. The parental crosses were of two kinds. In the first, temperature-sensitive mutants of HSV-1 and HSV-2 were crossed to produce wild-type recombinants. In the second, temperature-sensitive mutants of HSV-1 rendered resistant to phosphonoacetic acid were crossed with wild-type HSV-2, and recombinants that multiplied at nonpermissive temperature and were resistant to the drug were selected. The DNAs of the recombinants were mapped with XbaI, EcoRI, HpaI, HsuI, BglII, and, in some instances, KpnI restriction endonucleases. The results were as follows. We established the colinear arrangements of HSV-1 and HSV-2 DNAs. There was extensive interchange of genomic regions, ranging from the exchange of the entire L of S component of HSV DNA to substitutions of regions within the same component. In some recombinants, the reiterated sequences ab and ac bracketing the L and S components of HSV DNA were heterotypic. Most recombinants grew well and showed no obvious defects. The number of crossover events ranged from one to as many as six. Although crossover events occurred throughout the DNA, some clustering of crossover events was observed. Analysis of recombinants permitted localization of several markers used in this study and appears to be a useful technique for marker mapping. As previously reported, HSV DNA consists of four populations, differing in relative orientation of the L and S components. All recombinants could be displayed in one arrangement of L and S such that the number of crossover events was minimized. The data are consistent with the hypothesis that only one arrangement of the parental DNA participates in the generation of recombinants.

UR - http://www.scopus.com/inward/record.url?scp=0017665927&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017665927&partnerID=8YFLogxK

M3 - Article

C2 - 198577

AN - SCOPUS:0017665927

VL - 24

SP - 231

EP - 248

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 1

ER -