Anatomic site and immune function correlate with relative cytokine mRNA expression levels in lymphoid tissues of normal rhesus macaques

Kristina Abel, Michelle J. Alegria-Hartman, Kenneth Zanotto, Michael B. McChesney, Marta Marthas, Chris J Miller

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Reverse transcriptase real-time polymerase chain reaction was used to determine proinflammatory, anti-viral and immunoregulatory cytokine mRNA expression levels in peripheral blood mononuclear cells (PBMC) of healthy juvenile, adolescent and adult rhesus macaques. Few age-related changes in cytokine mRNA expression levels were observed. Expression of interleukin 2 and Mx, a type I interferon-inducible gene, decreased with age, whereas interleukin 4 and macrophage inflammatory protein 1 (MIP-1) alpha and beta mRNA levels increased in older monkeys. Independent of age, the pro-inflammatory cytokines [tumour necrosis factor alpha (TNF-α) and chemokines] were expressed at higher mRNA levels in PBMC than the immunoregulatory cytokines (interleukins 2, 4, 12). Pro-inflammatory cytokine mRNA expression levels were highest in lymphoid tissues draining mucosal surfaces. Thus, a correlation exists between cytokine mRNA levels in lymphoid tissues and the anatomical site.

Original languageEnglish (US)
Pages (from-to)191-204
Number of pages14
JournalCytokine
Volume16
Issue number5
DOIs
StatePublished - Dec 7 2001

Keywords

  • Age
  • Cytokine
  • Function
  • mRNA
  • Rhesus macaques
  • Tissue

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

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