Anaplasma phagocytophilum in zecche raccolte in parchi della regione Emilia-Romagna (Italia)

Translated title of the contribution: Anaplasma phagocytophilum in ticks from parks in the Emilia-Romagna region of northern Italy

Sara Aureli, Janet E Foley, Roberta Galuppi, Daniel Rejmanek, Cristina Bonoli, Maria Paola Tampieri

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The aim of this study was to assess the prevalence and phylogenetic characterisation of Anaplasma phagocytophilum in ticks in three parks of the Emilia-Romagna region of northern Italy, an area in which no survey of this agent had previously been conducted. A total of 360 tick samples were analysed; 292 were sourced from the environment and 68 from animals and humans. Real-time polymerase chain reaction revealed that 33 tick samples (9.2%) were positive for A. phagocytophilum. Ixodes ricinus was the only species found positive from the samples retrieved from the environment and was the most commonly infected among ticks removed from hosts. Sequence analysis of the 23S-5S rRNA gene performed on 23 samples revealed six variant sequences that differed by only a few nucleotides when compared to the GenBank sequences from humans, horse and small mammals. Msp4 gene sequences obtained from 7 samples were compared to those described in ruminants, especially roe deer (Capreolus capreolus) and goat isolates from different countries. The results of this study provided evidence of the circulation of A. phagocytophilum in the sites studied and indicated the possible involvement of wild ruminants. Additional studies that extend the sampling areas, or cover different sites, would contribute to a better understanding of the ecology and disease dynamics of A. phagocytophilum in northern Italy and would provide valuable information on zoonotic risks.

Original languageItalian
Pages (from-to)413-423
Number of pages11
JournalVeterinaria italiana
Volume48
Issue number4
StatePublished - Oct 2012

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Keywords

  • Anaplasma phagocytophilum
  • Anaplasmosis
  • Emilia-Romagna
  • Italy
  • Ixodes ricinus
  • Msp4
  • Polymerase chain reaction
  • RRNA
  • Tick

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Aureli, S., Foley, J. E., Galuppi, R., Rejmanek, D., Bonoli, C., & Tampieri, M. P. (2012). Anaplasma phagocytophilum in zecche raccolte in parchi della regione Emilia-Romagna (Italia). Veterinaria italiana, 48(4), 413-423.