Anaplasma phagocytophilum APH-1387 is expressed throughout bacterial intracellular development and localizes to the pathogen-occupied vacuolar membrane

Bernice Huang, Matthew J. Troese, Shaojing Ye, Jonathan T. Sims, Nathan L. Galloway, Dori L Borjesson, Jason A. Carlyon

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Obligate vacuolar pathogens produce proteins that localize to the host cell-derived membranes of the vacuoles in which they reside, yielding unique organelles that are optimally suited for pathogen survival. Anaplasma phagocytophilum is an obligate vacuolar bacterium that infects neutrophils and causes the emerging and potentially fatal disease human granulocytic anaplasmosis. Here we identified APH-1387 as the first A. phagocytophilum- derived protein that associates with the A. phagocytophilum-occupied vacuolar membrane (AVM). APH-1387, also referred to as P100, is a 61.4-kDa acidic protein that migrates with an apparent molecular weight of 115 kDa on SDS-PAGE gels. It carries 3 tandem direct repeats that comprise 58% of the protein. Each APH-1387 repeat carries a bilobed hydrophobic alpha-helix domain, which is a structural characteristic that is consistent with the structure of chlamydia-derived proteins that traverse inclusion membranes. APH-1387 is not detectable on the surfaces of A. phagocytophilum dense core organisms bound at the HL-60 cell surface, but abundant APH-1387 is detected on the surfaces of intravacuolar reticulate cell and dense core organisms. APH-1387 accumulates on the AVM throughout infection. It associates with the AVM in human HL-60, THP-1, and HMEC-1 cells and tick ISE6 cells. APH-1387 is expressed and localizes to the AVM in neutrophils recovered from A. phagocytophilum-infected mice. This paper presents the first direct evidence that A. phagocytophilum actively modifies its host cell-derived vacuole.

Original languageEnglish (US)
Pages (from-to)1864-1873
Number of pages10
JournalInfection and Immunity
Volume78
Issue number5
DOIs
StatePublished - May 2010

Fingerprint

Anaplasma phagocytophilum
Membranes
Vacuoles
Proteins
Neutrophils
Anaplasmosis
Tandem Repeat Sequences
Chlamydia
HL-60 Cells
Ticks
Organelles
Polyacrylamide Gel Electrophoresis
Molecular Weight
Gels
Cell Membrane
Bacteria
Survival
Infection

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Anaplasma phagocytophilum APH-1387 is expressed throughout bacterial intracellular development and localizes to the pathogen-occupied vacuolar membrane. / Huang, Bernice; Troese, Matthew J.; Ye, Shaojing; Sims, Jonathan T.; Galloway, Nathan L.; Borjesson, Dori L; Carlyon, Jason A.

In: Infection and Immunity, Vol. 78, No. 5, 05.2010, p. 1864-1873.

Research output: Contribution to journalArticle

Huang, Bernice ; Troese, Matthew J. ; Ye, Shaojing ; Sims, Jonathan T. ; Galloway, Nathan L. ; Borjesson, Dori L ; Carlyon, Jason A. / Anaplasma phagocytophilum APH-1387 is expressed throughout bacterial intracellular development and localizes to the pathogen-occupied vacuolar membrane. In: Infection and Immunity. 2010 ; Vol. 78, No. 5. pp. 1864-1873.
@article{a9be8b3300034de691d0b3fb131dbd04,
title = "Anaplasma phagocytophilum APH-1387 is expressed throughout bacterial intracellular development and localizes to the pathogen-occupied vacuolar membrane",
abstract = "Obligate vacuolar pathogens produce proteins that localize to the host cell-derived membranes of the vacuoles in which they reside, yielding unique organelles that are optimally suited for pathogen survival. Anaplasma phagocytophilum is an obligate vacuolar bacterium that infects neutrophils and causes the emerging and potentially fatal disease human granulocytic anaplasmosis. Here we identified APH-1387 as the first A. phagocytophilum- derived protein that associates with the A. phagocytophilum-occupied vacuolar membrane (AVM). APH-1387, also referred to as P100, is a 61.4-kDa acidic protein that migrates with an apparent molecular weight of 115 kDa on SDS-PAGE gels. It carries 3 tandem direct repeats that comprise 58{\%} of the protein. Each APH-1387 repeat carries a bilobed hydrophobic alpha-helix domain, which is a structural characteristic that is consistent with the structure of chlamydia-derived proteins that traverse inclusion membranes. APH-1387 is not detectable on the surfaces of A. phagocytophilum dense core organisms bound at the HL-60 cell surface, but abundant APH-1387 is detected on the surfaces of intravacuolar reticulate cell and dense core organisms. APH-1387 accumulates on the AVM throughout infection. It associates with the AVM in human HL-60, THP-1, and HMEC-1 cells and tick ISE6 cells. APH-1387 is expressed and localizes to the AVM in neutrophils recovered from A. phagocytophilum-infected mice. This paper presents the first direct evidence that A. phagocytophilum actively modifies its host cell-derived vacuole.",
author = "Bernice Huang and Troese, {Matthew J.} and Shaojing Ye and Sims, {Jonathan T.} and Galloway, {Nathan L.} and Borjesson, {Dori L} and Carlyon, {Jason A.}",
year = "2010",
month = "5",
doi = "10.1128/IAI.01418-09",
language = "English (US)",
volume = "78",
pages = "1864--1873",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "5",

}

TY - JOUR

T1 - Anaplasma phagocytophilum APH-1387 is expressed throughout bacterial intracellular development and localizes to the pathogen-occupied vacuolar membrane

AU - Huang, Bernice

AU - Troese, Matthew J.

AU - Ye, Shaojing

AU - Sims, Jonathan T.

AU - Galloway, Nathan L.

AU - Borjesson, Dori L

AU - Carlyon, Jason A.

PY - 2010/5

Y1 - 2010/5

N2 - Obligate vacuolar pathogens produce proteins that localize to the host cell-derived membranes of the vacuoles in which they reside, yielding unique organelles that are optimally suited for pathogen survival. Anaplasma phagocytophilum is an obligate vacuolar bacterium that infects neutrophils and causes the emerging and potentially fatal disease human granulocytic anaplasmosis. Here we identified APH-1387 as the first A. phagocytophilum- derived protein that associates with the A. phagocytophilum-occupied vacuolar membrane (AVM). APH-1387, also referred to as P100, is a 61.4-kDa acidic protein that migrates with an apparent molecular weight of 115 kDa on SDS-PAGE gels. It carries 3 tandem direct repeats that comprise 58% of the protein. Each APH-1387 repeat carries a bilobed hydrophobic alpha-helix domain, which is a structural characteristic that is consistent with the structure of chlamydia-derived proteins that traverse inclusion membranes. APH-1387 is not detectable on the surfaces of A. phagocytophilum dense core organisms bound at the HL-60 cell surface, but abundant APH-1387 is detected on the surfaces of intravacuolar reticulate cell and dense core organisms. APH-1387 accumulates on the AVM throughout infection. It associates with the AVM in human HL-60, THP-1, and HMEC-1 cells and tick ISE6 cells. APH-1387 is expressed and localizes to the AVM in neutrophils recovered from A. phagocytophilum-infected mice. This paper presents the first direct evidence that A. phagocytophilum actively modifies its host cell-derived vacuole.

AB - Obligate vacuolar pathogens produce proteins that localize to the host cell-derived membranes of the vacuoles in which they reside, yielding unique organelles that are optimally suited for pathogen survival. Anaplasma phagocytophilum is an obligate vacuolar bacterium that infects neutrophils and causes the emerging and potentially fatal disease human granulocytic anaplasmosis. Here we identified APH-1387 as the first A. phagocytophilum- derived protein that associates with the A. phagocytophilum-occupied vacuolar membrane (AVM). APH-1387, also referred to as P100, is a 61.4-kDa acidic protein that migrates with an apparent molecular weight of 115 kDa on SDS-PAGE gels. It carries 3 tandem direct repeats that comprise 58% of the protein. Each APH-1387 repeat carries a bilobed hydrophobic alpha-helix domain, which is a structural characteristic that is consistent with the structure of chlamydia-derived proteins that traverse inclusion membranes. APH-1387 is not detectable on the surfaces of A. phagocytophilum dense core organisms bound at the HL-60 cell surface, but abundant APH-1387 is detected on the surfaces of intravacuolar reticulate cell and dense core organisms. APH-1387 accumulates on the AVM throughout infection. It associates with the AVM in human HL-60, THP-1, and HMEC-1 cells and tick ISE6 cells. APH-1387 is expressed and localizes to the AVM in neutrophils recovered from A. phagocytophilum-infected mice. This paper presents the first direct evidence that A. phagocytophilum actively modifies its host cell-derived vacuole.

UR - http://www.scopus.com/inward/record.url?scp=77951215124&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77951215124&partnerID=8YFLogxK

U2 - 10.1128/IAI.01418-09

DO - 10.1128/IAI.01418-09

M3 - Article

C2 - 20212090

AN - SCOPUS:77951215124

VL - 78

SP - 1864

EP - 1873

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 5

ER -