Anaphase inactivation of the spindle checkpoint

William J. Palframan, Janet B. Meehl, Sue L. Jaspersen, Mark Winey, Andrew W. Murray

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

The spindle checkpoint delays cell cycle progression until microtubules attach each pair of sister chromosomes to opposite poles of the mitotic spindle. Following sister chromatid separation, however, the checkpoint ignores chromosomes whose kinetochores are attached to only one spindle pole, a state that activates the checkpoint prior to metaphase. We demonstrate that, in budding yeast, mutual inhibition between the anaphase-promoting complex (APC) and Mps1, an essential component of the checkpoint, leads to sustained inactivation of the spindle checkpoint. Mps1 protein abundance decreases in anaphase, and Mps1 is a target of the APC. Furthermore, expression of Mps1 in anaphase, or repression of the APC in anaphase, reactivates the spindle checkpoint. This APC-Mps1 feedback circuit allows cells to irreversibly inactivate the checkpoint during anaphase.

Original languageEnglish (US)
Pages (from-to)680-684
Number of pages5
JournalScience
Volume313
Issue number5787
DOIs
StatePublished - Aug 4 2006
Externally publishedYes

Fingerprint

Anaphase-Promoting Complex-Cyclosome
Anaphase
Chromosomes
Spindle Poles
Kinetochores
Spindle Apparatus
Saccharomycetales
Chromatids
Metaphase
Cell Cycle Checkpoints
Microtubules
Proteins

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Palframan, W. J., Meehl, J. B., Jaspersen, S. L., Winey, M., & Murray, A. W. (2006). Anaphase inactivation of the spindle checkpoint. Science, 313(5787), 680-684. https://doi.org/10.1126/science.1127205

Anaphase inactivation of the spindle checkpoint. / Palframan, William J.; Meehl, Janet B.; Jaspersen, Sue L.; Winey, Mark; Murray, Andrew W.

In: Science, Vol. 313, No. 5787, 04.08.2006, p. 680-684.

Research output: Contribution to journalArticle

Palframan, WJ, Meehl, JB, Jaspersen, SL, Winey, M & Murray, AW 2006, 'Anaphase inactivation of the spindle checkpoint', Science, vol. 313, no. 5787, pp. 680-684. https://doi.org/10.1126/science.1127205
Palframan WJ, Meehl JB, Jaspersen SL, Winey M, Murray AW. Anaphase inactivation of the spindle checkpoint. Science. 2006 Aug 4;313(5787):680-684. https://doi.org/10.1126/science.1127205
Palframan, William J. ; Meehl, Janet B. ; Jaspersen, Sue L. ; Winey, Mark ; Murray, Andrew W. / Anaphase inactivation of the spindle checkpoint. In: Science. 2006 ; Vol. 313, No. 5787. pp. 680-684.
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