Analyzing life-history traits and lipid storage using CARS microscopy for assessing effects of copper on the fitness of Caenorhabditis elegans

Hendrik Fueser, Nabil Majdi, Arne Haegerbaeumer, Christian Pilger, Henning Hachmeister, Paul Greife, Thomas R Huser, Walter Traunspurger

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Lipid storage provides energy for cell survival, growth, and reproduction and is closely related to the organismal response to stress imposed by toxic chemicals. However, the effects of toxicants on energy storage as it impacts certain life-history traits have rarely been investigated. Here, we used the nematode Caenorhabditis elegans as a test species for a chronic exposure to copper (Cu) at EC20 (0.50 mg Cu/l). Effects on the fatty acid distribution in C. elegans body were determined using coherent anti-Stokes Raman spectroscopy (CARS) to link population fitness responses with individual ecophysiological responses. Cu inhibited nematode reproductive capacity and offspring growth in addition to shortening the lifespan of exposed individuals. In adult nematodes, Cu exposure led to significant reduction of lipid storage compared to the Cu-free control: Under Cu, lipids filled only 0.5% of the nematode body volume vs. 7.5% in control nematodes, lipid droplets were on average 74% smaller and the number of tiny lipids (0–10 µm2) was increased. These results suggest that (1) Cu has an important effect on the life-history traits of nematodes; (2) the quantification of lipid storage can provide important information on the response of organisms to toxic stress; and (3) CARS microscopy is a promising tool for non-invasive quantitative and qualitative analyses of lipids as a measure of nematode fitness.

Original languageEnglish (US)
Pages (from-to)255-262
Number of pages8
JournalEcotoxicology and Environmental Safety
Volume156
DOIs
StatePublished - Jul 30 2018
Externally publishedYes

Fingerprint

Raman Spectrum Analysis
Caenorhabditis elegans
Lipids
Raman spectroscopy
Copper
Microscopy
Microscopic examination
Poisons
Energy storage
Growth
Cell growth
Reproduction
Life History Traits
Fatty acids
Cell Survival
Fatty Acids
Population

Keywords

  • Coherent anti-Stokes Raman scattering microscopy
  • Hanging-drop method
  • Heavy metal
  • Life-cycle
  • Lifespan
  • Lipid droplets

ASJC Scopus subject areas

  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Analyzing life-history traits and lipid storage using CARS microscopy for assessing effects of copper on the fitness of Caenorhabditis elegans. / Fueser, Hendrik; Majdi, Nabil; Haegerbaeumer, Arne; Pilger, Christian; Hachmeister, Henning; Greife, Paul; Huser, Thomas R; Traunspurger, Walter.

In: Ecotoxicology and Environmental Safety, Vol. 156, 30.07.2018, p. 255-262.

Research output: Contribution to journalArticle

Fueser, Hendrik ; Majdi, Nabil ; Haegerbaeumer, Arne ; Pilger, Christian ; Hachmeister, Henning ; Greife, Paul ; Huser, Thomas R ; Traunspurger, Walter. / Analyzing life-history traits and lipid storage using CARS microscopy for assessing effects of copper on the fitness of Caenorhabditis elegans. In: Ecotoxicology and Environmental Safety. 2018 ; Vol. 156. pp. 255-262.
@article{0d10637c1d814b5285f8e47b45077353,
title = "Analyzing life-history traits and lipid storage using CARS microscopy for assessing effects of copper on the fitness of Caenorhabditis elegans",
abstract = "Lipid storage provides energy for cell survival, growth, and reproduction and is closely related to the organismal response to stress imposed by toxic chemicals. However, the effects of toxicants on energy storage as it impacts certain life-history traits have rarely been investigated. Here, we used the nematode Caenorhabditis elegans as a test species for a chronic exposure to copper (Cu) at EC20 (0.50 mg Cu/l). Effects on the fatty acid distribution in C. elegans body were determined using coherent anti-Stokes Raman spectroscopy (CARS) to link population fitness responses with individual ecophysiological responses. Cu inhibited nematode reproductive capacity and offspring growth in addition to shortening the lifespan of exposed individuals. In adult nematodes, Cu exposure led to significant reduction of lipid storage compared to the Cu-free control: Under Cu, lipids filled only 0.5{\%} of the nematode body volume vs. 7.5{\%} in control nematodes, lipid droplets were on average 74{\%} smaller and the number of tiny lipids (0–10 µm2) was increased. These results suggest that (1) Cu has an important effect on the life-history traits of nematodes; (2) the quantification of lipid storage can provide important information on the response of organisms to toxic stress; and (3) CARS microscopy is a promising tool for non-invasive quantitative and qualitative analyses of lipids as a measure of nematode fitness.",
keywords = "Coherent anti-Stokes Raman scattering microscopy, Hanging-drop method, Heavy metal, Life-cycle, Lifespan, Lipid droplets",
author = "Hendrik Fueser and Nabil Majdi and Arne Haegerbaeumer and Christian Pilger and Henning Hachmeister and Paul Greife and Huser, {Thomas R} and Walter Traunspurger",
year = "2018",
month = "7",
day = "30",
doi = "10.1016/j.ecoenv.2018.03.037",
language = "English (US)",
volume = "156",
pages = "255--262",
journal = "Ecotoxicology and Environmental Safety",
issn = "0147-6513",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Analyzing life-history traits and lipid storage using CARS microscopy for assessing effects of copper on the fitness of Caenorhabditis elegans

AU - Fueser, Hendrik

AU - Majdi, Nabil

AU - Haegerbaeumer, Arne

AU - Pilger, Christian

AU - Hachmeister, Henning

AU - Greife, Paul

AU - Huser, Thomas R

AU - Traunspurger, Walter

PY - 2018/7/30

Y1 - 2018/7/30

N2 - Lipid storage provides energy for cell survival, growth, and reproduction and is closely related to the organismal response to stress imposed by toxic chemicals. However, the effects of toxicants on energy storage as it impacts certain life-history traits have rarely been investigated. Here, we used the nematode Caenorhabditis elegans as a test species for a chronic exposure to copper (Cu) at EC20 (0.50 mg Cu/l). Effects on the fatty acid distribution in C. elegans body were determined using coherent anti-Stokes Raman spectroscopy (CARS) to link population fitness responses with individual ecophysiological responses. Cu inhibited nematode reproductive capacity and offspring growth in addition to shortening the lifespan of exposed individuals. In adult nematodes, Cu exposure led to significant reduction of lipid storage compared to the Cu-free control: Under Cu, lipids filled only 0.5% of the nematode body volume vs. 7.5% in control nematodes, lipid droplets were on average 74% smaller and the number of tiny lipids (0–10 µm2) was increased. These results suggest that (1) Cu has an important effect on the life-history traits of nematodes; (2) the quantification of lipid storage can provide important information on the response of organisms to toxic stress; and (3) CARS microscopy is a promising tool for non-invasive quantitative and qualitative analyses of lipids as a measure of nematode fitness.

AB - Lipid storage provides energy for cell survival, growth, and reproduction and is closely related to the organismal response to stress imposed by toxic chemicals. However, the effects of toxicants on energy storage as it impacts certain life-history traits have rarely been investigated. Here, we used the nematode Caenorhabditis elegans as a test species for a chronic exposure to copper (Cu) at EC20 (0.50 mg Cu/l). Effects on the fatty acid distribution in C. elegans body were determined using coherent anti-Stokes Raman spectroscopy (CARS) to link population fitness responses with individual ecophysiological responses. Cu inhibited nematode reproductive capacity and offspring growth in addition to shortening the lifespan of exposed individuals. In adult nematodes, Cu exposure led to significant reduction of lipid storage compared to the Cu-free control: Under Cu, lipids filled only 0.5% of the nematode body volume vs. 7.5% in control nematodes, lipid droplets were on average 74% smaller and the number of tiny lipids (0–10 µm2) was increased. These results suggest that (1) Cu has an important effect on the life-history traits of nematodes; (2) the quantification of lipid storage can provide important information on the response of organisms to toxic stress; and (3) CARS microscopy is a promising tool for non-invasive quantitative and qualitative analyses of lipids as a measure of nematode fitness.

KW - Coherent anti-Stokes Raman scattering microscopy

KW - Hanging-drop method

KW - Heavy metal

KW - Life-cycle

KW - Lifespan

KW - Lipid droplets

UR - http://www.scopus.com/inward/record.url?scp=85043990957&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85043990957&partnerID=8YFLogxK

U2 - 10.1016/j.ecoenv.2018.03.037

DO - 10.1016/j.ecoenv.2018.03.037

M3 - Article

C2 - 29554610

AN - SCOPUS:85043990957

VL - 156

SP - 255

EP - 262

JO - Ecotoxicology and Environmental Safety

JF - Ecotoxicology and Environmental Safety

SN - 0147-6513

ER -