Analytical performance of accelerator mass spectrometry and liquid scintillation counting for detection of 14C-labeled atrazine metabolites in human urine

S. Douglass Gilman, Shirley J. Gee, Bruce D. Hammock, John S. Vogel, Kurt Haack, Bruce A. Buchholz, Stewart P H T Freeman, Ronald C. Wester, Xiaoying Hui, Howard I. Maibach

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

Accelerator mass spectrometry (AMS) has been applied to the detection of 14C-labeled urinary metabolites of the triazine herbicide, atrazine, and the analytical performance of AMS has been directly compared to that of liquid scintillation counting (LSC). Ten human subjects were given a dermal dose of 14C-labeled atrazine over 24 h, and urine from the subjects was collected over a 7-day period. Concentrations of 14C in the samples have been determined by AMS and LSC and range from 1.8 fmol/mL to 4.3 pmol/mL. Data from these two methods have a correlation coefficient of 0.998 for a linear plot of the entire sample set. Accelerator mass spectrometry provides superior concentration (2.2 vs 27 fmol/mL) and mass (5.5 vs 54 000 amol) detection limits relative to those of LSC for these samples. The precision of the data provided by AMS for low-level samples is 1.7%, and the day-to-day reproducibility of the AMS measurements is 3.9%. Factors limiting AMS detection limits for these samples and ways in which these can be improved are examined.

Original languageEnglish (US)
Pages (from-to)3463-3469
Number of pages7
JournalAnalytical Chemistry
Volume70
Issue number16
StatePublished - Aug 15 1998

ASJC Scopus subject areas

  • Analytical Chemistry

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