Abstract
Accelerator mass spectrometry (AMS) has been applied to the detection of 14C-labeled urinary metabolites of the triazine herbicide, atrazine, and the analytical performance of AMS has been directly compared to that of liquid scintillation counting (LSC). Ten human subjects were given a dermal dose of 14C-labeled atrazine over 24 h, and urine from the subjects was collected over a 7-day period. Concentrations of 14C in the samples have been determined by AMS and LSC and range from 1.8 fmol/mL to 4.3 pmol/mL. Data from these two methods have a correlation coefficient of 0.998 for a linear plot of the entire sample set. Accelerator mass spectrometry provides superior concentration (2.2 vs 27 fmol/mL) and mass (5.5 vs 54 000 amol) detection limits relative to those of LSC for these samples. The precision of the data provided by AMS for low-level samples is 1.7%, and the day-to-day reproducibility of the AMS measurements is 3.9%. Factors limiting AMS detection limits for these samples and ways in which these can be improved are examined.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3463-3469 |
| Number of pages | 7 |
| Journal | Analytical Chemistry |
| Volume | 70 |
| Issue number | 16 |
| State | Published - Aug 15 1998 |
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ASJC Scopus subject areas
- Analytical Chemistry
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Analytical performance of accelerator mass spectrometry and liquid scintillation counting for detection of 14C-labeled atrazine metabolites in human urine. / Gilman, S. Douglass; Gee, Shirley J.; Hammock, Bruce D.; Vogel, John S.; Haack, Kurt; Buchholz, Bruce A.; Freeman, Stewart P H T; Wester, Ronald C.; Hui, Xiaoying; Maibach, Howard I.
In: Analytical Chemistry, Vol. 70, No. 16, 15.08.1998, p. 3463-3469.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analytical performance of accelerator mass spectrometry and liquid scintillation counting for detection of 14C-labeled atrazine metabolites in human urine
AU - Gilman, S. Douglass
AU - Gee, Shirley J.
AU - Hammock, Bruce D.
AU - Vogel, John S.
AU - Haack, Kurt
AU - Buchholz, Bruce A.
AU - Freeman, Stewart P H T
AU - Wester, Ronald C.
AU - Hui, Xiaoying
AU - Maibach, Howard I.
PY - 1998/8/15
Y1 - 1998/8/15
N2 - Accelerator mass spectrometry (AMS) has been applied to the detection of 14C-labeled urinary metabolites of the triazine herbicide, atrazine, and the analytical performance of AMS has been directly compared to that of liquid scintillation counting (LSC). Ten human subjects were given a dermal dose of 14C-labeled atrazine over 24 h, and urine from the subjects was collected over a 7-day period. Concentrations of 14C in the samples have been determined by AMS and LSC and range from 1.8 fmol/mL to 4.3 pmol/mL. Data from these two methods have a correlation coefficient of 0.998 for a linear plot of the entire sample set. Accelerator mass spectrometry provides superior concentration (2.2 vs 27 fmol/mL) and mass (5.5 vs 54 000 amol) detection limits relative to those of LSC for these samples. The precision of the data provided by AMS for low-level samples is 1.7%, and the day-to-day reproducibility of the AMS measurements is 3.9%. Factors limiting AMS detection limits for these samples and ways in which these can be improved are examined.
AB - Accelerator mass spectrometry (AMS) has been applied to the detection of 14C-labeled urinary metabolites of the triazine herbicide, atrazine, and the analytical performance of AMS has been directly compared to that of liquid scintillation counting (LSC). Ten human subjects were given a dermal dose of 14C-labeled atrazine over 24 h, and urine from the subjects was collected over a 7-day period. Concentrations of 14C in the samples have been determined by AMS and LSC and range from 1.8 fmol/mL to 4.3 pmol/mL. Data from these two methods have a correlation coefficient of 0.998 for a linear plot of the entire sample set. Accelerator mass spectrometry provides superior concentration (2.2 vs 27 fmol/mL) and mass (5.5 vs 54 000 amol) detection limits relative to those of LSC for these samples. The precision of the data provided by AMS for low-level samples is 1.7%, and the day-to-day reproducibility of the AMS measurements is 3.9%. Factors limiting AMS detection limits for these samples and ways in which these can be improved are examined.
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M3 - Article
C2 - 9726169
AN - SCOPUS:0032529441
VL - 70
SP - 3463
EP - 3469
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 16
ER -