Analytical performance of accelerator mass spectrometry and liquid scintillation counting for detection of 14C-labeled atrazine metabolites in human urine

S. Douglass Gilman, Shirley J. Gee, Bruce D. Hammock, John S. Vogel, Kurt Haack, Bruce A. Buchholz, Stewart P H T Freeman, Ronald C. Wester, Xiaoying Hui, Howard I. Maibach

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Abstract

Accelerator mass spectrometry (AMS) has been applied to the detection of 14C-labeled urinary metabolites of the triazine herbicide, atrazine, and the analytical performance of AMS has been directly compared to that of liquid scintillation counting (LSC). Ten human subjects were given a dermal dose of 14C-labeled atrazine over 24 h, and urine from the subjects was collected over a 7-day period. Concentrations of 14C in the samples have been determined by AMS and LSC and range from 1.8 fmol/mL to 4.3 pmol/mL. Data from these two methods have a correlation coefficient of 0.998 for a linear plot of the entire sample set. Accelerator mass spectrometry provides superior concentration (2.2 vs 27 fmol/mL) and mass (5.5 vs 54 000 amol) detection limits relative to those of LSC for these samples. The precision of the data provided by AMS for low-level samples is 1.7%, and the day-to-day reproducibility of the AMS measurements is 3.9%. Factors limiting AMS detection limits for these samples and ways in which these can be improved are examined.

Original languageEnglish (US)
Pages (from-to)3463-3469
Number of pages7
JournalAnalytical Chemistry
Volume70
Issue number16
StatePublished - Aug 15 1998

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Atrazine
Scintillation
Metabolites
Particle accelerators
Mass spectrometry
Liquids
Triazines
Herbicides

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Gilman, S. D., Gee, S. J., Hammock, B. D., Vogel, J. S., Haack, K., Buchholz, B. A., ... Maibach, H. I. (1998). Analytical performance of accelerator mass spectrometry and liquid scintillation counting for detection of 14C-labeled atrazine metabolites in human urine. Analytical Chemistry, 70(16), 3463-3469.

Analytical performance of accelerator mass spectrometry and liquid scintillation counting for detection of 14C-labeled atrazine metabolites in human urine. / Gilman, S. Douglass; Gee, Shirley J.; Hammock, Bruce D.; Vogel, John S.; Haack, Kurt; Buchholz, Bruce A.; Freeman, Stewart P H T; Wester, Ronald C.; Hui, Xiaoying; Maibach, Howard I.

In: Analytical Chemistry, Vol. 70, No. 16, 15.08.1998, p. 3463-3469.

Research output: Contribution to journalArticle

Gilman, SD, Gee, SJ, Hammock, BD, Vogel, JS, Haack, K, Buchholz, BA, Freeman, SPHT, Wester, RC, Hui, X & Maibach, HI 1998, 'Analytical performance of accelerator mass spectrometry and liquid scintillation counting for detection of 14C-labeled atrazine metabolites in human urine', Analytical Chemistry, vol. 70, no. 16, pp. 3463-3469.
Gilman, S. Douglass ; Gee, Shirley J. ; Hammock, Bruce D. ; Vogel, John S. ; Haack, Kurt ; Buchholz, Bruce A. ; Freeman, Stewart P H T ; Wester, Ronald C. ; Hui, Xiaoying ; Maibach, Howard I. / Analytical performance of accelerator mass spectrometry and liquid scintillation counting for detection of 14C-labeled atrazine metabolites in human urine. In: Analytical Chemistry. 1998 ; Vol. 70, No. 16. pp. 3463-3469.
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