Analytical magnetapheresis of ferritin-labeled lymphocytes

Maciej Zborowski, Chwan Bor Fuh, Ralph Green, Liping Sun, Ralph Green

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Analytical magnetapheresis is a technique for analyzing magnetic particles in suspension. The magnetically susceptible particles form a deposition pattern from the suspending medium under carefully controlled flow and magnetic field conditions. This technique was used to determine the effective magnetic volumetric susceptibility, Δχ of human lymphocytes labeled with an iron-rich protein, ferritin. Dynabeads M450, monodisperse polymeric beads doped with magnetite, of a diameter 4.5 μm, close to that of human lymphocytes, were used as a reference. The experiment showed an almost complete deposition of ferritin-labeled lymphocytes at an average flow velocity of 0.28 mm/s, a representative magnetic field of 1.67 T, and a magnetic field gradient of 2.57 T/mm. The calculated Δχ was (2.92 ± 0.24) × 10-6[SI] (ferritin-labeled lymphocytes), and the corresponding number of ferritin molecules per lymphocyte was (1.75 ± 0.44) × 107. In comparison, an almost complete deposition of the Dynabeads was observed at a much higher average flow velocity, 15 mm/s, a much lower field, 0.164 T, and a much lower field gradient, 0.025 T/mm. These results corresponded to a much higher Δχ = 0.245[SI] (Dynabeads M450). These results offer important guidelines in evaluating the use of ferritin as a soluble magnetic cell label.

Original languageEnglish (US)
Pages (from-to)3702-3712
Number of pages11
JournalAnalytical Chemistry
Volume67
Issue number20
StatePublished - Oct 15 1995
Externally publishedYes

Fingerprint

Lymphocytes
Ferritins
Magnetic fields
Flow velocity
Ferrosoferric Oxide
Labels
Flow fields
Suspensions
Iron
Molecules
Proteins
Experiments

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Zborowski, M., Fuh, C. B., Green, R., Sun, L., & Green, R. (1995). Analytical magnetapheresis of ferritin-labeled lymphocytes. Analytical Chemistry, 67(20), 3702-3712.

Analytical magnetapheresis of ferritin-labeled lymphocytes. / Zborowski, Maciej; Fuh, Chwan Bor; Green, Ralph; Sun, Liping; Green, Ralph.

In: Analytical Chemistry, Vol. 67, No. 20, 15.10.1995, p. 3702-3712.

Research output: Contribution to journalArticle

Zborowski, M, Fuh, CB, Green, R, Sun, L & Green, R 1995, 'Analytical magnetapheresis of ferritin-labeled lymphocytes', Analytical Chemistry, vol. 67, no. 20, pp. 3702-3712.
Zborowski M, Fuh CB, Green R, Sun L, Green R. Analytical magnetapheresis of ferritin-labeled lymphocytes. Analytical Chemistry. 1995 Oct 15;67(20):3702-3712.
Zborowski, Maciej ; Fuh, Chwan Bor ; Green, Ralph ; Sun, Liping ; Green, Ralph. / Analytical magnetapheresis of ferritin-labeled lymphocytes. In: Analytical Chemistry. 1995 ; Vol. 67, No. 20. pp. 3702-3712.
@article{8edd90c247de4483a84d41f6c74cee98,
title = "Analytical magnetapheresis of ferritin-labeled lymphocytes",
abstract = "Analytical magnetapheresis is a technique for analyzing magnetic particles in suspension. The magnetically susceptible particles form a deposition pattern from the suspending medium under carefully controlled flow and magnetic field conditions. This technique was used to determine the effective magnetic volumetric susceptibility, Δχ of human lymphocytes labeled with an iron-rich protein, ferritin. Dynabeads M450, monodisperse polymeric beads doped with magnetite, of a diameter 4.5 μm, close to that of human lymphocytes, were used as a reference. The experiment showed an almost complete deposition of ferritin-labeled lymphocytes at an average flow velocity of 0.28 mm/s, a representative magnetic field of 1.67 T, and a magnetic field gradient of 2.57 T/mm. The calculated Δχ was (2.92 ± 0.24) × 10-6[SI] (ferritin-labeled lymphocytes), and the corresponding number of ferritin molecules per lymphocyte was (1.75 ± 0.44) × 107. In comparison, an almost complete deposition of the Dynabeads was observed at a much higher average flow velocity, 15 mm/s, a much lower field, 0.164 T, and a much lower field gradient, 0.025 T/mm. These results corresponded to a much higher Δχ = 0.245[SI] (Dynabeads M450). These results offer important guidelines in evaluating the use of ferritin as a soluble magnetic cell label.",
author = "Maciej Zborowski and Fuh, {Chwan Bor} and Ralph Green and Liping Sun and Ralph Green",
year = "1995",
month = "10",
day = "15",
language = "English (US)",
volume = "67",
pages = "3702--3712",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "20",

}

TY - JOUR

T1 - Analytical magnetapheresis of ferritin-labeled lymphocytes

AU - Zborowski, Maciej

AU - Fuh, Chwan Bor

AU - Green, Ralph

AU - Sun, Liping

AU - Green, Ralph

PY - 1995/10/15

Y1 - 1995/10/15

N2 - Analytical magnetapheresis is a technique for analyzing magnetic particles in suspension. The magnetically susceptible particles form a deposition pattern from the suspending medium under carefully controlled flow and magnetic field conditions. This technique was used to determine the effective magnetic volumetric susceptibility, Δχ of human lymphocytes labeled with an iron-rich protein, ferritin. Dynabeads M450, monodisperse polymeric beads doped with magnetite, of a diameter 4.5 μm, close to that of human lymphocytes, were used as a reference. The experiment showed an almost complete deposition of ferritin-labeled lymphocytes at an average flow velocity of 0.28 mm/s, a representative magnetic field of 1.67 T, and a magnetic field gradient of 2.57 T/mm. The calculated Δχ was (2.92 ± 0.24) × 10-6[SI] (ferritin-labeled lymphocytes), and the corresponding number of ferritin molecules per lymphocyte was (1.75 ± 0.44) × 107. In comparison, an almost complete deposition of the Dynabeads was observed at a much higher average flow velocity, 15 mm/s, a much lower field, 0.164 T, and a much lower field gradient, 0.025 T/mm. These results corresponded to a much higher Δχ = 0.245[SI] (Dynabeads M450). These results offer important guidelines in evaluating the use of ferritin as a soluble magnetic cell label.

AB - Analytical magnetapheresis is a technique for analyzing magnetic particles in suspension. The magnetically susceptible particles form a deposition pattern from the suspending medium under carefully controlled flow and magnetic field conditions. This technique was used to determine the effective magnetic volumetric susceptibility, Δχ of human lymphocytes labeled with an iron-rich protein, ferritin. Dynabeads M450, monodisperse polymeric beads doped with magnetite, of a diameter 4.5 μm, close to that of human lymphocytes, were used as a reference. The experiment showed an almost complete deposition of ferritin-labeled lymphocytes at an average flow velocity of 0.28 mm/s, a representative magnetic field of 1.67 T, and a magnetic field gradient of 2.57 T/mm. The calculated Δχ was (2.92 ± 0.24) × 10-6[SI] (ferritin-labeled lymphocytes), and the corresponding number of ferritin molecules per lymphocyte was (1.75 ± 0.44) × 107. In comparison, an almost complete deposition of the Dynabeads was observed at a much higher average flow velocity, 15 mm/s, a much lower field, 0.164 T, and a much lower field gradient, 0.025 T/mm. These results corresponded to a much higher Δχ = 0.245[SI] (Dynabeads M450). These results offer important guidelines in evaluating the use of ferritin as a soluble magnetic cell label.

UR - http://www.scopus.com/inward/record.url?scp=0029645611&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029645611&partnerID=8YFLogxK

M3 - Article

C2 - 8644920

AN - SCOPUS:0029645611

VL - 67

SP - 3702

EP - 3712

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 20

ER -