Analysis of whole blood manganese by flameless atomic absorption spectrophotometry and its use as an indicator of manganese status in animals

Michael S. Clegg, Bo Lönnerdal, Lucille S. Hurley, Carl L Keen

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

To investigate the use of whole blood manganese (Mn) as an indicator of total body Mn, we measured Mn in whole blood and liver of rats fed purified diets containing adequate (45 μg Mn/g diet) or deficient (1 μg Mn/g diet) Mn. The mean hepatic Mn concentration was significantly lower (P < 0.001) in the Mn-deficient group compared to the control group, 0.36, μg Mn/g and 1.73 μg Mn/g, respectively. Furthermore, whole blood Mn was significantly reduced (P < 0.001) in the deficient group when compared to the control group, 4.0 ng Mn/ml and 8.6 ng Mn/ml, respectively. Hepatic Mn linearly regressed against whole blood Mn yielded a statistically significant (P < 0.001) correlation coefficient of 0.775. These data suggest that whole blood Mn is a valid indicator of body Mn status and thus may be useful, in addition to the measurements of serum copper and zinc, for the diagnosis and prognosis of diseases in which the metabolism of trace elements is affected. In addition, this paper describes and delineates operational parameters for the measurement of whole blood Mn using the IL 551 atomic absorption spectrophotometer and the IL 555 B flameless atomizer.

Original languageEnglish (US)
Pages (from-to)12-18
Number of pages7
JournalAnalytical Biochemistry
Volume157
Issue number1
DOIs
StatePublished - Aug 15 1986

Keywords

  • atomic absorption
  • manganese
  • Mn
  • nutritional status
  • trace elements

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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