Analysis of trace elements in animal tissues - III. Determination of managanese by graphite furnace atomic absorption spectrophotometry

Michael S. Clegg, Carl L Keen, Bo Lonnerdal, Lucille S. Hurley

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Graphite furnace atomic absorption spectrophotometry is a method used for the measurement of low concentrations of manganese (ppb range). Despite the widespread use of this technique, there is considerable inconsistency concerning sample preparation and choice of instrumental parameters. In this paper, we determined manganese concentrations of National Bureau of Standards (NBS) bovine liver by both graphite furnace (Instrumentation Laboratory IL 555B) and flame atomic absorption following wet digestion of the sample with nitric acid. The following instrumental parameters for the graphite furnace were found optimal for the measurement of manganese in digested NBS bovine liver: inert gas flow=14 SCFH, drying temperature 100°C/15 s (step 1), 125°C/15 s (step 2), pyrolysis temperature 500°C/15 s (step 3), and 1000°C/15 s (step 4); atomization temperature 2250°C/10 s (step 5). For optimal results, the nitric acid concentration of the sample should be between 2 and 4 M. There were no significant differences found for manganese concentrations determined by either peak height or peak area measurement. Additionally, no significant differences were found in manganese concentrations determined by flame or furnace methods. Assuming proper sample preparation and choice of instrumental parameters, values obtained for manganese concentration by graphite furnace and flame atomic absorption spectrophotometry are similar. Therefore, data obtained by these two methods can be compared directly.

Original languageEnglish (US)
Pages (from-to)145-156
Number of pages12
JournalBiological Trace Element Research
Issue number2-3
StatePublished - Jun 1982


  • analysis of biological trace elements
  • atomic absorption spectrophotometry, of Mn in animal tissues
  • biological samples
  • biotrace element analysis, of Mn
  • instrumental parameters, in biological trace element analysis
  • Manganese determination, by graphite furnace AAS
  • methods in biological trace element analysis

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Biochemistry, Genetics and Molecular Biology(all)


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