Analysis of tissue-specific methylation patterns of mouse mammary tumor virus DNA by two-dimensional Southern blotting

T. G. Fanning, W. S. Hu, Robert Cardiff

Research output: Contribution to journalArticle

9 Scopus citations


We used a two-dimensional Southern blotting procedure to analyze the tissue-specific methylation patterns of the five endogenous mouse mammary viruses in the GR/A mouse strain. Our findings suggest that in certain tissues (brain, kidney, and liver) all proviruses are extensively methylated. In other tissues (spleen, placenta, and testes) all proviruses are hypomethylated to some degree. In these tissues individual proviruses display both quantitative and qualitative differences in methylation. We interpret the general patterns of tissue-specific hypomethylation in terms of a 'hitch-hiker' model: mouse mammary tumor virus proviral methylation patterns reflect the tissue-specific activity of neighboring sequences. The observation that certain sites on particular proviruses are differentially methylated in a tissue-specific fashion may reflect tissue-specific differences in the makeup or conformation, or both, of proviral-containing chromatin.

Original languageEnglish (US)
Pages (from-to)726-730
Number of pages5
JournalJournal of Virology
Issue number3
StatePublished - Jan 1 1985


ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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