Analysis of the Rhesus Cytomegalovirus Immediate-Early Gene Promoter

Donald J. Alcendor, Peter A Barry, Elissa Pratt-Lowe, Paul A Luciw

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

A cytomegalovirus (CMV) isolate has been recovered from a rhesus macaque (Macaca mulatta) experimentally infected with simian immunodeficiency virus. The full-length immediate-early gene (IE) was cloned from a genomic library of this primary rhesus CMV isolate (RhCMV) using the African green monkey (AGM) CMV IE gene as probe. The promoter region and the first non-coding exon of the RhCMV IE gene have been sequenced. Nucleotide sequence comparisons from positions -733 to +625 (relative to the start site of transcription at +1) reveal 70 and 48% sequence homology with the AGM CMVIE and the human CMV (HCMV) IE genes, respectively. A region that includes the TATA box, the cis repression signal and the first exon in the RhCMV IE gene sequence is 91 and 76% homologous with the AGM CMV and HCMV IE genes, respectively. In permissive MRC-5 cells infected with RhCMV, synthesis of RNA from the IE gene peaks at 4-8 hr post infection. These studies provide a basis for elucidating molecular mechanisms regulating expression of the RhCMV IE gene and viral replication.

Original languageEnglish (US)
Pages (from-to)815-821
Number of pages7
JournalVirology
Volume194
Issue number2
DOIs
StatePublished - Jun 1993

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

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