Analysis of the mechanisms of mitochondrial NADH regulation in cardiac trabeculae

Rolf Brandes, Donald M Bers

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

We have previously shown that increased cardiac work initially caused a rapid Ca2+-independent fall of mitochondrial [NADH] ([NADH](m)) to a minimum level, and this was followed by a slow Ca2+-dependent recovery toward control level (Brandes and Bers, Biophys. J. 71:1024-1035, 1996; Brandes and Bers, Circ. Res. 80:82-87, 1997). The purpose of this study is to improve our understanding of the factors that control [NADH](m) during increased work. [NADH](m) was monitored using fluorescence spectroscopy in intact rat trabeculae isolated from the right ventricular wall. Work was increased by increasing sarcomere length, pacing frequency, external [Ca2+], or by decreased temperature. The results were: 1) The initial fall of [NADH](m) during increased pacing frequency depends independently on increased myofilament work and on increased Ca2+-transport ATPase activity. 2) The [NADH](m) recovery process depends on average cytosolic [Ca2+] (Av[Ca2+](c), but not on absolute work level. 3) The initial fall of [NADH](m) and the [NADH](m) recovery are similar whether increased work is associated with low frequency and high Ca2+-transient amplitude or vice versa (at the same myofilament work level and Av[Ca2+](c). 4) The mechanisms associated with the smaller fall and recovery of [NADH](m) at 37°C versus 27°C, may be explained by lowered Av[Ca2+](c) and myofilament work. The NADH control mechanisms that operate at lower temperature are thus qualitatively similar at more physiological temperatures.

Original languageEnglish (US)
Pages (from-to)1666-1682
Number of pages17
JournalBiophysical Journal
Volume77
Issue number3
StatePublished - Sep 1999
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics

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