Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species

R. Brodersen, F. Bijlsma, K. Gori, K. T. Jensen, W. Chen, J. Dominguez, K. Haverson, Peter F Moore, A. Saalmüller, D. Sachs, W. J. Slierendrecht, C. Stokes, O. Vainio, F. Zuckermann, B. Aasted

Research output: Contribution to journalArticle

82 Scopus citations

Abstract

213 Monoclonal antibodies (mAbs) raised against leucocyte surface antigens from human and 11 animal species were analyzed for reactivities against leucocytes from human and 15 different animal species. We found 77 mAbs (36%) to cross-react. Altogether, 217 cross reactions were registered out of 3195 possible combinations (7%). Most of the cross reacting mAbs had integrin or MHC class II specificities. This study defined cross reactions an the following markers: CD 1a, 1c, 2, 4, 5, 8, 9, 11a, 11b, 14, 18, 20, 21, 23, 29, 31, 41, 43, 44, 45, 45R, 46, 49, 61, 62L, TCR γ/δ, BCR, Thy-1, MHC class I and MHC class II, Swine-WC7 and Cattle-WC1. In order to characterize the molecular weight (MW) of the corresponding cross reacting antigens, selected mAbs were used to immunoprecipitate the antigens. The MW's of the analyzed precipitated antigens were in good agreement with the MWs of the homologous antigens. The followed strategy was found to be efficient and economical in defining new leucocyte antigen reactive mAbs.

Original languageEnglish (US)
Pages (from-to)1-13
Number of pages13
JournalVeterinary Immunology and Immunopathology
Volume64
Issue number1
DOIs
StatePublished - Jun 30 1998

Keywords

  • Cat
  • Cattle
  • Chicken
  • Cross reaction
  • Dog
  • Goat
  • Guinea pig
  • Horse
  • Human
  • Leucocyte antigens
  • Mink
  • Monoclonal antibodies
  • Rabbit
  • Sheep
  • Swine
  • Toad
  • Trout
  • Turtle

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

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    Brodersen, R., Bijlsma, F., Gori, K., Jensen, K. T., Chen, W., Dominguez, J., Haverson, K., Moore, P. F., Saalmüller, A., Sachs, D., Slierendrecht, W. J., Stokes, C., Vainio, O., Zuckermann, F., & Aasted, B. (1998). Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species. Veterinary Immunology and Immunopathology, 64(1), 1-13. https://doi.org/10.1016/S0165-2427(98)00117-2