Analysis of the genetic variation in growth, ecophysiology, and chemical and metabolomic composition of wood of Populus trichocarpa provenances

Fernando P. Guerra, James H. Richards, Oliver Fiehn, Randi Famula, Brian J. Stanton, Richard Shuren, Robert Sykes, Mark F. Davis, David B. Neale

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Populus trichocarpa is a biological model and a candidate species for bioethanol production. Although intraspecific variation is recognized, knowledge about genetic variation underlying the properties of its lignocellulosic biomass is still incomplete. Genetic variation is fundamental for continuing genetic improvement. In this study, we carried out a comprehensive phenotypic characterization of this species, analyzing a suite of quantitative traits associated with growth performance and wood quality. Traits involved growth rate (height, diameter), phenology (bud flush), and ecophysiology (leaf carbon and nitrogen content and isotopic composition), along with the chemical composition (contents of sugars and lignin) and metabolome of wood. We utilized 460 clones, representing 101 provenances collected from Oregon and Washington. These genotypes were planted in California, in 2009, and sampled after three growing seasons. Trait characterization was carried out by direct measurements, determination of stable isotopes (leaf samples), and technologies based on mass spectrometry (wood samples). A significant clonal effect was observed for most of the traits, explaining up to 76.4 % of total variation. Estimates of “broad-sense heritability” were moderate to high, reaching 0.96 (for date of bud flush). Phenotypic and genetic correlations varied extensively depending on specific traits. In addition, metabolomic analyses quantified 632 metabolites. Twenty-eight of these varied significantly with experimental factors, showing low to moderate heritability and correlation estimates. The results support the presence of significant clonal variation and inheritance for the assessed traits, required for response to genetic selection.

Original languageEnglish (US)
Article number6
Pages (from-to)1-16
Number of pages16
JournalTree Genetics and Genomes
Volume12
Issue number1
DOIs
StatePublished - Feb 1 2016

Fingerprint

Populus
ecophysiology
Populus balsamifera subsp. trichocarpa
Metabolomics
metabolomics
provenance
genetic variation
heritability
buds
clonal variation
bud
metabolome
wood quality
phenotypic correlation
growth traits
ethanol production
Growth
quantitative traits
sugar content
genetic correlation

Keywords

  • Cellulose
  • Growth
  • Lignin
  • Populus trichocarpa
  • Stable isotopes
  • Wood metabolome

ASJC Scopus subject areas

  • Forestry
  • Horticulture
  • Genetics
  • Molecular Biology

Cite this

Analysis of the genetic variation in growth, ecophysiology, and chemical and metabolomic composition of wood of Populus trichocarpa provenances. / Guerra, Fernando P.; Richards, James H.; Fiehn, Oliver; Famula, Randi; Stanton, Brian J.; Shuren, Richard; Sykes, Robert; Davis, Mark F.; Neale, David B.

In: Tree Genetics and Genomes, Vol. 12, No. 1, 6, 01.02.2016, p. 1-16.

Research output: Contribution to journalArticle

Guerra, FP, Richards, JH, Fiehn, O, Famula, R, Stanton, BJ, Shuren, R, Sykes, R, Davis, MF & Neale, DB 2016, 'Analysis of the genetic variation in growth, ecophysiology, and chemical and metabolomic composition of wood of Populus trichocarpa provenances', Tree Genetics and Genomes, vol. 12, no. 1, 6, pp. 1-16. https://doi.org/10.1007/s11295-015-0965-8
Guerra, Fernando P. ; Richards, James H. ; Fiehn, Oliver ; Famula, Randi ; Stanton, Brian J. ; Shuren, Richard ; Sykes, Robert ; Davis, Mark F. ; Neale, David B. / Analysis of the genetic variation in growth, ecophysiology, and chemical and metabolomic composition of wood of Populus trichocarpa provenances. In: Tree Genetics and Genomes. 2016 ; Vol. 12, No. 1. pp. 1-16.
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