Analysis of the frequencies and of the memory T cell phenotypes of human CD8+ T cells specific for influenza A viruses

Xiaosong He, Kutubuddin Mahmood, Holden T. Maecker, Tyson H. Holmes, George W. Kemble, Ann M. Arvin, Harry B. Greenberg

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

We characterized the human CD8+ T cell response against influenza A viruses by a flow cytometry-based assay. Peripheral blood mononuclear cells (PBMCs) were incubated with inactivated influenza virus preparation, for 17 h, and were stained for intracellular interferon-γ. Major histocompatibility complex class I-restricted memory CD8+ T cells specific for influenza antigens were detected in PBMCs from all 19 adult donors, at an average frequency of 0.39%. On average, 83% of influenza virus-specific CD8+ T cells expressed the differentiation-associated marker CD27, a percentage that is significantly higher than that of CD8+ T cells specific for pp65 of human cytomegalovirus (53%). These observations indicate that class I-restricted immunity against influenza A viruses is characterized by the persistence, after clearance of infection, of circulating antigen-specific CD8+ T cells. The different patterns of CD27 expression in influenza virus- and cytomegalovirus-specific CD8+ T cells suggest that influenza virus-specific memory and effector CD8+ T cells can be differentiated by phenotypic analysis.

Original languageEnglish (US)
Pages (from-to)1075-1084
Number of pages10
JournalJournal of Infectious Diseases
Volume187
Issue number7
DOIs
StatePublished - Apr 1 2003
Externally publishedYes

Fingerprint

Influenza A virus
T-Lymphocytes
Phenotype
Orthomyxoviridae
Cytomegalovirus
Blood Cells
CD8 Antigens
Differentiation Antigens
Major Histocompatibility Complex
Interferons
Human Influenza
Cell Differentiation
Immunity
Flow Cytometry
Tissue Donors
Antigens
Infection

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

He, X., Mahmood, K., Maecker, H. T., Holmes, T. H., Kemble, G. W., Arvin, A. M., & Greenberg, H. B. (2003). Analysis of the frequencies and of the memory T cell phenotypes of human CD8+ T cells specific for influenza A viruses. Journal of Infectious Diseases, 187(7), 1075-1084. https://doi.org/10.1086/368218

Analysis of the frequencies and of the memory T cell phenotypes of human CD8+ T cells specific for influenza A viruses. / He, Xiaosong; Mahmood, Kutubuddin; Maecker, Holden T.; Holmes, Tyson H.; Kemble, George W.; Arvin, Ann M.; Greenberg, Harry B.

In: Journal of Infectious Diseases, Vol. 187, No. 7, 01.04.2003, p. 1075-1084.

Research output: Contribution to journalArticle

He, X, Mahmood, K, Maecker, HT, Holmes, TH, Kemble, GW, Arvin, AM & Greenberg, HB 2003, 'Analysis of the frequencies and of the memory T cell phenotypes of human CD8+ T cells specific for influenza A viruses', Journal of Infectious Diseases, vol. 187, no. 7, pp. 1075-1084. https://doi.org/10.1086/368218
He, Xiaosong ; Mahmood, Kutubuddin ; Maecker, Holden T. ; Holmes, Tyson H. ; Kemble, George W. ; Arvin, Ann M. ; Greenberg, Harry B. / Analysis of the frequencies and of the memory T cell phenotypes of human CD8+ T cells specific for influenza A viruses. In: Journal of Infectious Diseases. 2003 ; Vol. 187, No. 7. pp. 1075-1084.
@article{afa0f493fded4ab48c6ee6ae6a4041b1,
title = "Analysis of the frequencies and of the memory T cell phenotypes of human CD8+ T cells specific for influenza A viruses",
abstract = "We characterized the human CD8+ T cell response against influenza A viruses by a flow cytometry-based assay. Peripheral blood mononuclear cells (PBMCs) were incubated with inactivated influenza virus preparation, for 17 h, and were stained for intracellular interferon-γ. Major histocompatibility complex class I-restricted memory CD8+ T cells specific for influenza antigens were detected in PBMCs from all 19 adult donors, at an average frequency of 0.39{\%}. On average, 83{\%} of influenza virus-specific CD8+ T cells expressed the differentiation-associated marker CD27, a percentage that is significantly higher than that of CD8+ T cells specific for pp65 of human cytomegalovirus (53{\%}). These observations indicate that class I-restricted immunity against influenza A viruses is characterized by the persistence, after clearance of infection, of circulating antigen-specific CD8+ T cells. The different patterns of CD27 expression in influenza virus- and cytomegalovirus-specific CD8+ T cells suggest that influenza virus-specific memory and effector CD8+ T cells can be differentiated by phenotypic analysis.",
author = "Xiaosong He and Kutubuddin Mahmood and Maecker, {Holden T.} and Holmes, {Tyson H.} and Kemble, {George W.} and Arvin, {Ann M.} and Greenberg, {Harry B.}",
year = "2003",
month = "4",
day = "1",
doi = "10.1086/368218",
language = "English (US)",
volume = "187",
pages = "1075--1084",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "7",

}

TY - JOUR

T1 - Analysis of the frequencies and of the memory T cell phenotypes of human CD8+ T cells specific for influenza A viruses

AU - He, Xiaosong

AU - Mahmood, Kutubuddin

AU - Maecker, Holden T.

AU - Holmes, Tyson H.

AU - Kemble, George W.

AU - Arvin, Ann M.

AU - Greenberg, Harry B.

PY - 2003/4/1

Y1 - 2003/4/1

N2 - We characterized the human CD8+ T cell response against influenza A viruses by a flow cytometry-based assay. Peripheral blood mononuclear cells (PBMCs) were incubated with inactivated influenza virus preparation, for 17 h, and were stained for intracellular interferon-γ. Major histocompatibility complex class I-restricted memory CD8+ T cells specific for influenza antigens were detected in PBMCs from all 19 adult donors, at an average frequency of 0.39%. On average, 83% of influenza virus-specific CD8+ T cells expressed the differentiation-associated marker CD27, a percentage that is significantly higher than that of CD8+ T cells specific for pp65 of human cytomegalovirus (53%). These observations indicate that class I-restricted immunity against influenza A viruses is characterized by the persistence, after clearance of infection, of circulating antigen-specific CD8+ T cells. The different patterns of CD27 expression in influenza virus- and cytomegalovirus-specific CD8+ T cells suggest that influenza virus-specific memory and effector CD8+ T cells can be differentiated by phenotypic analysis.

AB - We characterized the human CD8+ T cell response against influenza A viruses by a flow cytometry-based assay. Peripheral blood mononuclear cells (PBMCs) were incubated with inactivated influenza virus preparation, for 17 h, and were stained for intracellular interferon-γ. Major histocompatibility complex class I-restricted memory CD8+ T cells specific for influenza antigens were detected in PBMCs from all 19 adult donors, at an average frequency of 0.39%. On average, 83% of influenza virus-specific CD8+ T cells expressed the differentiation-associated marker CD27, a percentage that is significantly higher than that of CD8+ T cells specific for pp65 of human cytomegalovirus (53%). These observations indicate that class I-restricted immunity against influenza A viruses is characterized by the persistence, after clearance of infection, of circulating antigen-specific CD8+ T cells. The different patterns of CD27 expression in influenza virus- and cytomegalovirus-specific CD8+ T cells suggest that influenza virus-specific memory and effector CD8+ T cells can be differentiated by phenotypic analysis.

UR - http://www.scopus.com/inward/record.url?scp=0345270018&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0345270018&partnerID=8YFLogxK

U2 - 10.1086/368218

DO - 10.1086/368218

M3 - Article

C2 - 12660922

AN - SCOPUS:0345270018

VL - 187

SP - 1075

EP - 1084

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 7

ER -