Analysis of the frequencies and of the memory T cell phenotypes of human CD8+ T cells specific for influenza A viruses

Xiaosong He, Kutubuddin Mahmood, Holden T. Maecker, Tyson H. Holmes, George W. Kemble, Ann M. Arvin, Harry B. Greenberg

Research output: Contribution to journalArticle

58 Scopus citations

Abstract

We characterized the human CD8+ T cell response against influenza A viruses by a flow cytometry-based assay. Peripheral blood mononuclear cells (PBMCs) were incubated with inactivated influenza virus preparation, for 17 h, and were stained for intracellular interferon-γ. Major histocompatibility complex class I-restricted memory CD8+ T cells specific for influenza antigens were detected in PBMCs from all 19 adult donors, at an average frequency of 0.39%. On average, 83% of influenza virus-specific CD8+ T cells expressed the differentiation-associated marker CD27, a percentage that is significantly higher than that of CD8+ T cells specific for pp65 of human cytomegalovirus (53%). These observations indicate that class I-restricted immunity against influenza A viruses is characterized by the persistence, after clearance of infection, of circulating antigen-specific CD8+ T cells. The different patterns of CD27 expression in influenza virus- and cytomegalovirus-specific CD8+ T cells suggest that influenza virus-specific memory and effector CD8+ T cells can be differentiated by phenotypic analysis.

Original languageEnglish (US)
Pages (from-to)1075-1084
Number of pages10
JournalJournal of Infectious Diseases
Volume187
Issue number7
DOIs
StatePublished - Apr 1 2003
Externally publishedYes

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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    He, X., Mahmood, K., Maecker, H. T., Holmes, T. H., Kemble, G. W., Arvin, A. M., & Greenberg, H. B. (2003). Analysis of the frequencies and of the memory T cell phenotypes of human CD8+ T cells specific for influenza A viruses. Journal of Infectious Diseases, 187(7), 1075-1084. https://doi.org/10.1086/368218