Abstract
We characterized the human CD8+ T cell response against influenza A viruses by a flow cytometry-based assay. Peripheral blood mononuclear cells (PBMCs) were incubated with inactivated influenza virus preparation, for 17 h, and were stained for intracellular interferon-γ. Major histocompatibility complex class I-restricted memory CD8+ T cells specific for influenza antigens were detected in PBMCs from all 19 adult donors, at an average frequency of 0.39%. On average, 83% of influenza virus-specific CD8+ T cells expressed the differentiation-associated marker CD27, a percentage that is significantly higher than that of CD8+ T cells specific for pp65 of human cytomegalovirus (53%). These observations indicate that class I-restricted immunity against influenza A viruses is characterized by the persistence, after clearance of infection, of circulating antigen-specific CD8+ T cells. The different patterns of CD27 expression in influenza virus- and cytomegalovirus-specific CD8+ T cells suggest that influenza virus-specific memory and effector CD8+ T cells can be differentiated by phenotypic analysis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1075-1084 |
| Number of pages | 10 |
| Journal | Journal of Infectious Diseases |
| Volume | 187 |
| Issue number | 7 |
| DOIs | |
| State | Published - Apr 1 2003 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Public Health, Environmental and Occupational Health
- Immunology
Cite this
Analysis of the frequencies and of the memory T cell phenotypes of human CD8+ T cells specific for influenza A viruses. / He, Xiaosong; Mahmood, Kutubuddin; Maecker, Holden T.; Holmes, Tyson H.; Kemble, George W.; Arvin, Ann M.; Greenberg, Harry B.
In: Journal of Infectious Diseases, Vol. 187, No. 7, 01.04.2003, p. 1075-1084.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of the frequencies and of the memory T cell phenotypes of human CD8+ T cells specific for influenza A viruses
AU - He, Xiaosong
AU - Mahmood, Kutubuddin
AU - Maecker, Holden T.
AU - Holmes, Tyson H.
AU - Kemble, George W.
AU - Arvin, Ann M.
AU - Greenberg, Harry B.
PY - 2003/4/1
Y1 - 2003/4/1
N2 - We characterized the human CD8+ T cell response against influenza A viruses by a flow cytometry-based assay. Peripheral blood mononuclear cells (PBMCs) were incubated with inactivated influenza virus preparation, for 17 h, and were stained for intracellular interferon-γ. Major histocompatibility complex class I-restricted memory CD8+ T cells specific for influenza antigens were detected in PBMCs from all 19 adult donors, at an average frequency of 0.39%. On average, 83% of influenza virus-specific CD8+ T cells expressed the differentiation-associated marker CD27, a percentage that is significantly higher than that of CD8+ T cells specific for pp65 of human cytomegalovirus (53%). These observations indicate that class I-restricted immunity against influenza A viruses is characterized by the persistence, after clearance of infection, of circulating antigen-specific CD8+ T cells. The different patterns of CD27 expression in influenza virus- and cytomegalovirus-specific CD8+ T cells suggest that influenza virus-specific memory and effector CD8+ T cells can be differentiated by phenotypic analysis.
AB - We characterized the human CD8+ T cell response against influenza A viruses by a flow cytometry-based assay. Peripheral blood mononuclear cells (PBMCs) were incubated with inactivated influenza virus preparation, for 17 h, and were stained for intracellular interferon-γ. Major histocompatibility complex class I-restricted memory CD8+ T cells specific for influenza antigens were detected in PBMCs from all 19 adult donors, at an average frequency of 0.39%. On average, 83% of influenza virus-specific CD8+ T cells expressed the differentiation-associated marker CD27, a percentage that is significantly higher than that of CD8+ T cells specific for pp65 of human cytomegalovirus (53%). These observations indicate that class I-restricted immunity against influenza A viruses is characterized by the persistence, after clearance of infection, of circulating antigen-specific CD8+ T cells. The different patterns of CD27 expression in influenza virus- and cytomegalovirus-specific CD8+ T cells suggest that influenza virus-specific memory and effector CD8+ T cells can be differentiated by phenotypic analysis.
UR - http://www.scopus.com/inward/record.url?scp=0345270018&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0345270018&partnerID=8YFLogxK
U2 - 10.1086/368218
DO - 10.1086/368218
M3 - Article
C2 - 12660922
AN - SCOPUS:0345270018
VL - 187
SP - 1075
EP - 1084
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 7
ER -