Abstract
Wild type equine p53 was amplified between exons 2 and 9 by the polymerase chain reaction using primers designed from conserved regions in other species. An 828 base pair region, corresponding to codons 25-313 of human p53, was sequenced in both directions. Human and equine amino acid sequences were 87% homologous in this region and 96% homologous in conserved domains II-V. Of eight equine cutaneous or mucocutaneous squamous cell carcinomas directly sequenced from exons 5-8, two had p53 point mutations resulting in single amino acid substitutions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 125-130 |
| Number of pages | 6 |
| Journal | Cancer Letters |
| Volume | 107 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 1 1996 |
Keywords
- Horse
- p53
- Polymerase chain reaction
- Sequencing
- Squamous cell carcinoma
ASJC Scopus subject areas
- Cancer Research
- Molecular Biology
- Oncology
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Cite this
Pazzi, K. A., Kraegel, S. A., Griffey, S. M., Theon, A. P., & Madewell, B. R. (1996). Analysis of the equine tumor suppressor gene p53 in the normal horse and in eight cutaneous squamous cell carcinomas. Cancer Letters, 107(1), 125-130. https://doi.org/10.1016/0304-3835(96)04359-5