Abstract
Wild type equine p53 was amplified between exons 2 and 9 by the polymerase chain reaction using primers designed from conserved regions in other species. An 828 base pair region, corresponding to codons 25-313 of human p53, was sequenced in both directions. Human and equine amino acid sequences were 87% homologous in this region and 96% homologous in conserved domains II-V. Of eight equine cutaneous or mucocutaneous squamous cell carcinomas directly sequenced from exons 5-8, two had p53 point mutations resulting in single amino acid substitutions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 125-130 |
| Number of pages | 6 |
| Journal | Cancer Letters |
| Volume | 107 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 1 1996 |
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Keywords
- Horse
- p53
- Polymerase chain reaction
- Sequencing
- Squamous cell carcinoma
ASJC Scopus subject areas
- Cancer Research
- Molecular Biology
- Oncology
Cite this
Analysis of the equine tumor suppressor gene p53 in the normal horse and in eight cutaneous squamous cell carcinomas. / Pazzi, Karen A.; Kraegel, Susan A.; Griffey, Stephen M; Theon, Alain P; Madewell, Bruce R.
In: Cancer Letters, Vol. 107, No. 1, 01.10.1996, p. 125-130.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of the equine tumor suppressor gene p53 in the normal horse and in eight cutaneous squamous cell carcinomas
AU - Pazzi, Karen A.
AU - Kraegel, Susan A.
AU - Griffey, Stephen M
AU - Theon, Alain P
AU - Madewell, Bruce R.
PY - 1996/10/1
Y1 - 1996/10/1
N2 - Wild type equine p53 was amplified between exons 2 and 9 by the polymerase chain reaction using primers designed from conserved regions in other species. An 828 base pair region, corresponding to codons 25-313 of human p53, was sequenced in both directions. Human and equine amino acid sequences were 87% homologous in this region and 96% homologous in conserved domains II-V. Of eight equine cutaneous or mucocutaneous squamous cell carcinomas directly sequenced from exons 5-8, two had p53 point mutations resulting in single amino acid substitutions.
AB - Wild type equine p53 was amplified between exons 2 and 9 by the polymerase chain reaction using primers designed from conserved regions in other species. An 828 base pair region, corresponding to codons 25-313 of human p53, was sequenced in both directions. Human and equine amino acid sequences were 87% homologous in this region and 96% homologous in conserved domains II-V. Of eight equine cutaneous or mucocutaneous squamous cell carcinomas directly sequenced from exons 5-8, two had p53 point mutations resulting in single amino acid substitutions.
KW - Horse
KW - p53
KW - Polymerase chain reaction
KW - Sequencing
KW - Squamous cell carcinoma
UR - http://www.scopus.com/inward/record.url?scp=0030272545&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030272545&partnerID=8YFLogxK
U2 - 10.1016/0304-3835(96)04359-5
DO - 10.1016/0304-3835(96)04359-5
M3 - Article
C2 - 8913276
AN - SCOPUS:0030272545
VL - 107
SP - 125
EP - 130
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 1
ER -