Abstract
Wild type equine p53 was amplified between exons 2 and 9 by the polymerase chain reaction using primers designed from conserved regions in other species. An 828 base pair region, corresponding to codons 25-313 of human p53, was sequenced in both directions. Human and equine amino acid sequences were 87% homologous in this region and 96% homologous in conserved domains II-V. Of eight equine cutaneous or mucocutaneous squamous cell carcinomas directly sequenced from exons 5-8, two had p53 point mutations resulting in single amino acid substitutions.
Original language | English (US) |
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Pages (from-to) | 125-130 |
Number of pages | 6 |
Journal | Cancer Letters |
Volume | 107 |
Issue number | 1 |
DOIs | |
State | Published - Oct 1 1996 |
Keywords
- Horse
- p53
- Polymerase chain reaction
- Sequencing
- Squamous cell carcinoma
ASJC Scopus subject areas
- Cancer Research
- Molecular Biology
- Oncology