Analysis of the equine tumor suppressor gene p53 in the normal horse and in eight cutaneous squamous cell carcinomas

Karen A. Pazzi, Susan A. Kraegel, Stephen M Griffey, Alain P Theon, Bruce R. Madewell

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Wild type equine p53 was amplified between exons 2 and 9 by the polymerase chain reaction using primers designed from conserved regions in other species. An 828 base pair region, corresponding to codons 25-313 of human p53, was sequenced in both directions. Human and equine amino acid sequences were 87% homologous in this region and 96% homologous in conserved domains II-V. Of eight equine cutaneous or mucocutaneous squamous cell carcinomas directly sequenced from exons 5-8, two had p53 point mutations resulting in single amino acid substitutions.

Original languageEnglish (US)
Pages (from-to)125-130
Number of pages6
JournalCancer Letters
Volume107
Issue number1
DOIs
StatePublished - Oct 1 1996

Keywords

  • Horse
  • p53
  • Polymerase chain reaction
  • Sequencing
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

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