Analysis of the behavioral activity of C- and N-terminal fragments of cholecystokinin octapeptide

Jacqueline Crawley, S. St-Pierre, P. Gaudreau

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Cholecystokinin (CCK) is a gut peptide which induces a syndrome of satiety, including reduced food intake and reduced exploratory behaviors. To investigate the minimal active sequence within the octapeptide molecule for reducing exploratory behavior, all C- and N-terminal fragments of CCK 26-33 (CCK8) were synthesized. Only CCK26-33 [H-Asp-Tyr(SO3H)-Met-Gly-Trp-Met-Asp-Phe-NH2] and CCK27-33 [H-Tyr(SO3H)-Met-Gly-Trp-Met-Asp-Phe-NH2] induced the behavioral effects within a physiological dose range. Smaller fragments as well as unsulfated CCK26-33 and CCK27-33 were inactive in doses as high as 10-3 mol/kg. The requirement of the entire C-terminal heptapeptide for the behavioral effects of CCK is contrasted against the activity of smaller fragments at pancreatic, gastrointestinal and brain CCK receptors.

Original languageEnglish (US)
Pages (from-to)438-444
Number of pages7
JournalJournal of Pharmacology and Experimental Therapeutics
Volume230
Issue number2
StatePublished - 1984
Externally publishedYes

Fingerprint

Sincalide
Exploratory Behavior
Cholecystokinin
Cholecystokinin Receptors
Eating
Peptides
Brain
cholecystokinin hexapeptide
cholecystokinin (26-33)

ASJC Scopus subject areas

  • Pharmacology

Cite this

Analysis of the behavioral activity of C- and N-terminal fragments of cholecystokinin octapeptide. / Crawley, Jacqueline; St-Pierre, S.; Gaudreau, P.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 230, No. 2, 1984, p. 438-444.

Research output: Contribution to journalArticle

@article{93916086e73746abbbebaac987cc89f2,
title = "Analysis of the behavioral activity of C- and N-terminal fragments of cholecystokinin octapeptide",
abstract = "Cholecystokinin (CCK) is a gut peptide which induces a syndrome of satiety, including reduced food intake and reduced exploratory behaviors. To investigate the minimal active sequence within the octapeptide molecule for reducing exploratory behavior, all C- and N-terminal fragments of CCK 26-33 (CCK8) were synthesized. Only CCK26-33 [H-Asp-Tyr(SO3H)-Met-Gly-Trp-Met-Asp-Phe-NH2] and CCK27-33 [H-Tyr(SO3H)-Met-Gly-Trp-Met-Asp-Phe-NH2] induced the behavioral effects within a physiological dose range. Smaller fragments as well as unsulfated CCK26-33 and CCK27-33 were inactive in doses as high as 10-3 mol/kg. The requirement of the entire C-terminal heptapeptide for the behavioral effects of CCK is contrasted against the activity of smaller fragments at pancreatic, gastrointestinal and brain CCK receptors.",
author = "Jacqueline Crawley and S. St-Pierre and P. Gaudreau",
year = "1984",
language = "English (US)",
volume = "230",
pages = "438--444",
journal = "Journal of Pharmacology and Experimental Therapeutics",
issn = "0022-3565",
publisher = "American Society for Pharmacology and Experimental Therapeutics",
number = "2",

}

TY - JOUR

T1 - Analysis of the behavioral activity of C- and N-terminal fragments of cholecystokinin octapeptide

AU - Crawley, Jacqueline

AU - St-Pierre, S.

AU - Gaudreau, P.

PY - 1984

Y1 - 1984

N2 - Cholecystokinin (CCK) is a gut peptide which induces a syndrome of satiety, including reduced food intake and reduced exploratory behaviors. To investigate the minimal active sequence within the octapeptide molecule for reducing exploratory behavior, all C- and N-terminal fragments of CCK 26-33 (CCK8) were synthesized. Only CCK26-33 [H-Asp-Tyr(SO3H)-Met-Gly-Trp-Met-Asp-Phe-NH2] and CCK27-33 [H-Tyr(SO3H)-Met-Gly-Trp-Met-Asp-Phe-NH2] induced the behavioral effects within a physiological dose range. Smaller fragments as well as unsulfated CCK26-33 and CCK27-33 were inactive in doses as high as 10-3 mol/kg. The requirement of the entire C-terminal heptapeptide for the behavioral effects of CCK is contrasted against the activity of smaller fragments at pancreatic, gastrointestinal and brain CCK receptors.

AB - Cholecystokinin (CCK) is a gut peptide which induces a syndrome of satiety, including reduced food intake and reduced exploratory behaviors. To investigate the minimal active sequence within the octapeptide molecule for reducing exploratory behavior, all C- and N-terminal fragments of CCK 26-33 (CCK8) were synthesized. Only CCK26-33 [H-Asp-Tyr(SO3H)-Met-Gly-Trp-Met-Asp-Phe-NH2] and CCK27-33 [H-Tyr(SO3H)-Met-Gly-Trp-Met-Asp-Phe-NH2] induced the behavioral effects within a physiological dose range. Smaller fragments as well as unsulfated CCK26-33 and CCK27-33 were inactive in doses as high as 10-3 mol/kg. The requirement of the entire C-terminal heptapeptide for the behavioral effects of CCK is contrasted against the activity of smaller fragments at pancreatic, gastrointestinal and brain CCK receptors.

UR - http://www.scopus.com/inward/record.url?scp=0021142369&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021142369&partnerID=8YFLogxK

M3 - Article

VL - 230

SP - 438

EP - 444

JO - Journal of Pharmacology and Experimental Therapeutics

JF - Journal of Pharmacology and Experimental Therapeutics

SN - 0022-3565

IS - 2

ER -