Abstract
Since the CALUX (Chemically Activated LUciferase gene eXpression) bioassay is a fast, sensitive and inexpensive tool for the analysis of a high number of samples, validation of new methods is urgently needed. In this study, a new method for the analysis of PCDD/Fs and dioxin-like PCBs in atmospheric deposition samples with the CALUX bioassay was developed, optimized and validated. The method consists of 4 steps: filtration, extraction, clean up and bioassay analysis. To avoid the use of large amounts of toxic solvents, new techniques were used for filtration and extraction: a C18 filter was used instead of a liquid/liquid extraction and an Accelerated Solvent Extractor (ASE) was used instead of the traditional soxhlet extraction. After pre-oxidation of the sample extract, clean up was done using a multi-layer silica gel column coupled to a carbon column. The PCDD/F and PCB fractions were finally analyzed with the H1L7.5c1 and/or the H1L6.1c3 mouse hepatoma cell lines. The limit of quantification was 1.4pg CALUX-BEQm-2d-1 for the PCBs and 5.6pgCALUX-BEQm-2d-1 for the PCDD/Fs, when using the new sensitive H1L7.5c1 cell line. The GC-HRMS recovery for all PCDD/F congeners was between 55% and 112%, with a mean recovery of 90%. CALUX recoveries of spiked procedural blanks were between the accepted ranges of 80-120%. Repeatability and reproducibility were satisfactory and no interferences from metals were detected. The first results from the Flemish measurement program showed good correlation between CALUX and GC-HRMS.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 718-724 |
| Number of pages | 7 |
| Journal | Chemosphere |
| Volume | 82 |
| Issue number | 5 |
| DOIs | |
| State | Published - Jan 2011 |
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Keywords
- Atmospheric depositions
- CALUX
- Dioxine-like PCBs
- PCDD/Fs
- Validation
ASJC Scopus subject areas
- Environmental Chemistry
- Chemistry(all)
Cite this
Analysis of PCDD/Fs and dioxin-like PCBs in atmospheric deposition samples from the Flemish measurement network : Optimization and validation of a new CALUX bioassay method. / Croes, K.; Van Langenhove, K.; Elskens, M.; Desmedt, M.; Roekens, E.; Kotz, A.; Denison, M. S.; Baeyens, W.
In: Chemosphere, Vol. 82, No. 5, 01.2011, p. 718-724.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of PCDD/Fs and dioxin-like PCBs in atmospheric deposition samples from the Flemish measurement network
T2 - Optimization and validation of a new CALUX bioassay method
AU - Croes, K.
AU - Van Langenhove, K.
AU - Elskens, M.
AU - Desmedt, M.
AU - Roekens, E.
AU - Kotz, A.
AU - Denison, M. S.
AU - Baeyens, W.
PY - 2011/1
Y1 - 2011/1
N2 - Since the CALUX (Chemically Activated LUciferase gene eXpression) bioassay is a fast, sensitive and inexpensive tool for the analysis of a high number of samples, validation of new methods is urgently needed. In this study, a new method for the analysis of PCDD/Fs and dioxin-like PCBs in atmospheric deposition samples with the CALUX bioassay was developed, optimized and validated. The method consists of 4 steps: filtration, extraction, clean up and bioassay analysis. To avoid the use of large amounts of toxic solvents, new techniques were used for filtration and extraction: a C18 filter was used instead of a liquid/liquid extraction and an Accelerated Solvent Extractor (ASE) was used instead of the traditional soxhlet extraction. After pre-oxidation of the sample extract, clean up was done using a multi-layer silica gel column coupled to a carbon column. The PCDD/F and PCB fractions were finally analyzed with the H1L7.5c1 and/or the H1L6.1c3 mouse hepatoma cell lines. The limit of quantification was 1.4pg CALUX-BEQm-2d-1 for the PCBs and 5.6pgCALUX-BEQm-2d-1 for the PCDD/Fs, when using the new sensitive H1L7.5c1 cell line. The GC-HRMS recovery for all PCDD/F congeners was between 55% and 112%, with a mean recovery of 90%. CALUX recoveries of spiked procedural blanks were between the accepted ranges of 80-120%. Repeatability and reproducibility were satisfactory and no interferences from metals were detected. The first results from the Flemish measurement program showed good correlation between CALUX and GC-HRMS.
AB - Since the CALUX (Chemically Activated LUciferase gene eXpression) bioassay is a fast, sensitive and inexpensive tool for the analysis of a high number of samples, validation of new methods is urgently needed. In this study, a new method for the analysis of PCDD/Fs and dioxin-like PCBs in atmospheric deposition samples with the CALUX bioassay was developed, optimized and validated. The method consists of 4 steps: filtration, extraction, clean up and bioassay analysis. To avoid the use of large amounts of toxic solvents, new techniques were used for filtration and extraction: a C18 filter was used instead of a liquid/liquid extraction and an Accelerated Solvent Extractor (ASE) was used instead of the traditional soxhlet extraction. After pre-oxidation of the sample extract, clean up was done using a multi-layer silica gel column coupled to a carbon column. The PCDD/F and PCB fractions were finally analyzed with the H1L7.5c1 and/or the H1L6.1c3 mouse hepatoma cell lines. The limit of quantification was 1.4pg CALUX-BEQm-2d-1 for the PCBs and 5.6pgCALUX-BEQm-2d-1 for the PCDD/Fs, when using the new sensitive H1L7.5c1 cell line. The GC-HRMS recovery for all PCDD/F congeners was between 55% and 112%, with a mean recovery of 90%. CALUX recoveries of spiked procedural blanks were between the accepted ranges of 80-120%. Repeatability and reproducibility were satisfactory and no interferences from metals were detected. The first results from the Flemish measurement program showed good correlation between CALUX and GC-HRMS.
KW - Atmospheric depositions
KW - CALUX
KW - Dioxine-like PCBs
KW - PCDD/Fs
KW - Validation
UR - http://www.scopus.com/inward/record.url?scp=78650735477&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78650735477&partnerID=8YFLogxK
U2 - 10.1016/j.chemosphere.2010.10.092
DO - 10.1016/j.chemosphere.2010.10.092
M3 - Article
C2 - 21094512
AN - SCOPUS:78650735477
VL - 82
SP - 718
EP - 724
JO - Chemosphere
JF - Chemosphere
SN - 0045-6535
IS - 5
ER -