Analysis of hepatic T lymphocyte and immunoglobulin deposits in patients with primary biliary cirrhosis

Sheri M. Krams, Judith A Van de Water, Ross L. Coppel, Carlos Esquivel, John Roberts, Aftab Ansari, M. Eric Gershwin

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

The histological findings in patients with primary biliary cirrhosis have been well-defined and are often used in the clinical staging of disease. However, it has only been with the development of reagents that phenotypically characterize the lymphoid infiltrate that attempts have been made to correlate pathophysiology with immune effector populations. Indeed, the inflammatory hepatic lesions in primary biliary cirrhosis have been described as containing CD4-positive and CDS-positive T cells. Less clear, however, have been the T cell receptors in these lesions. Further, the data on immunoglobulin deposits in hepatic lesions have been less well-defined; this deficit may be a result of the quality of polyspecific sera and difficulties in background. To address these issues, we have used a battery of well-defined monospecific and polyspecific reagents to phenotypically define the occurrence of lymphoid cells in the livers of patients undergoing transplantation. Furthermore, we have defined these same markers on T cell lines derived from liver, regional lymph node and peripheral blood. The predominant cell type in the mononuclear infiltrate is the CD3+, CD4+ T lymphocyte bearing the T cell receptor αβ. T cell lines from the same patients demonstrate similar findings. Of special importance, however, was the detection of CD20+ B cells and Ig+ cells in the lymphoid infiltrate. Indeed, we also readily demonstrated the presence of immunoglobulin on the surface of biliary epithelium. These data suggest that mechanisms involved in the pathophysiology of primary biliary cirrhosis may include both T cell and antibody mechanisms. The results also underscore the need to develop a functional, and not just a phenotypical, assay of the inflammatory infiltrate.

Original languageEnglish (US)
Pages (from-to)306-313
Number of pages8
JournalHepatology
Volume12
Issue number2
StatePublished - Aug 1990

Fingerprint

Biliary Liver Cirrhosis
Immunoglobulins
T-Lymphocytes
Liver
T-Cell Antigen Receptor
Lymphocytes
Cell Line
B-Cell Antigen Receptors
B-Lymphocytes
Epithelium
Transplantation
Lymph Nodes
Antibodies
Serum
Population

ASJC Scopus subject areas

  • Hepatology

Cite this

Analysis of hepatic T lymphocyte and immunoglobulin deposits in patients with primary biliary cirrhosis. / Krams, Sheri M.; Van de Water, Judith A; Coppel, Ross L.; Esquivel, Carlos; Roberts, John; Ansari, Aftab; Gershwin, M. Eric.

In: Hepatology, Vol. 12, No. 2, 08.1990, p. 306-313.

Research output: Contribution to journalArticle

Krams, SM, Van de Water, JA, Coppel, RL, Esquivel, C, Roberts, J, Ansari, A & Gershwin, ME 1990, 'Analysis of hepatic T lymphocyte and immunoglobulin deposits in patients with primary biliary cirrhosis', Hepatology, vol. 12, no. 2, pp. 306-313.
Krams SM, Van de Water JA, Coppel RL, Esquivel C, Roberts J, Ansari A et al. Analysis of hepatic T lymphocyte and immunoglobulin deposits in patients with primary biliary cirrhosis. Hepatology. 1990 Aug;12(2):306-313.
Krams, Sheri M. ; Van de Water, Judith A ; Coppel, Ross L. ; Esquivel, Carlos ; Roberts, John ; Ansari, Aftab ; Gershwin, M. Eric. / Analysis of hepatic T lymphocyte and immunoglobulin deposits in patients with primary biliary cirrhosis. In: Hepatology. 1990 ; Vol. 12, No. 2. pp. 306-313.
@article{e497822fd9bb45b095003c20415d85ec,
title = "Analysis of hepatic T lymphocyte and immunoglobulin deposits in patients with primary biliary cirrhosis",
abstract = "The histological findings in patients with primary biliary cirrhosis have been well-defined and are often used in the clinical staging of disease. However, it has only been with the development of reagents that phenotypically characterize the lymphoid infiltrate that attempts have been made to correlate pathophysiology with immune effector populations. Indeed, the inflammatory hepatic lesions in primary biliary cirrhosis have been described as containing CD4-positive and CDS-positive T cells. Less clear, however, have been the T cell receptors in these lesions. Further, the data on immunoglobulin deposits in hepatic lesions have been less well-defined; this deficit may be a result of the quality of polyspecific sera and difficulties in background. To address these issues, we have used a battery of well-defined monospecific and polyspecific reagents to phenotypically define the occurrence of lymphoid cells in the livers of patients undergoing transplantation. Furthermore, we have defined these same markers on T cell lines derived from liver, regional lymph node and peripheral blood. The predominant cell type in the mononuclear infiltrate is the CD3+, CD4+ T lymphocyte bearing the T cell receptor αβ. T cell lines from the same patients demonstrate similar findings. Of special importance, however, was the detection of CD20+ B cells and Ig+ cells in the lymphoid infiltrate. Indeed, we also readily demonstrated the presence of immunoglobulin on the surface of biliary epithelium. These data suggest that mechanisms involved in the pathophysiology of primary biliary cirrhosis may include both T cell and antibody mechanisms. The results also underscore the need to develop a functional, and not just a phenotypical, assay of the inflammatory infiltrate.",
author = "Krams, {Sheri M.} and {Van de Water}, {Judith A} and Coppel, {Ross L.} and Carlos Esquivel and John Roberts and Aftab Ansari and Gershwin, {M. Eric}",
year = "1990",
month = "8",
language = "English (US)",
volume = "12",
pages = "306--313",
journal = "Hepatology",
issn = "0270-9139",
publisher = "John Wiley and Sons Ltd",
number = "2",

}

TY - JOUR

T1 - Analysis of hepatic T lymphocyte and immunoglobulin deposits in patients with primary biliary cirrhosis

AU - Krams, Sheri M.

AU - Van de Water, Judith A

AU - Coppel, Ross L.

AU - Esquivel, Carlos

AU - Roberts, John

AU - Ansari, Aftab

AU - Gershwin, M. Eric

PY - 1990/8

Y1 - 1990/8

N2 - The histological findings in patients with primary biliary cirrhosis have been well-defined and are often used in the clinical staging of disease. However, it has only been with the development of reagents that phenotypically characterize the lymphoid infiltrate that attempts have been made to correlate pathophysiology with immune effector populations. Indeed, the inflammatory hepatic lesions in primary biliary cirrhosis have been described as containing CD4-positive and CDS-positive T cells. Less clear, however, have been the T cell receptors in these lesions. Further, the data on immunoglobulin deposits in hepatic lesions have been less well-defined; this deficit may be a result of the quality of polyspecific sera and difficulties in background. To address these issues, we have used a battery of well-defined monospecific and polyspecific reagents to phenotypically define the occurrence of lymphoid cells in the livers of patients undergoing transplantation. Furthermore, we have defined these same markers on T cell lines derived from liver, regional lymph node and peripheral blood. The predominant cell type in the mononuclear infiltrate is the CD3+, CD4+ T lymphocyte bearing the T cell receptor αβ. T cell lines from the same patients demonstrate similar findings. Of special importance, however, was the detection of CD20+ B cells and Ig+ cells in the lymphoid infiltrate. Indeed, we also readily demonstrated the presence of immunoglobulin on the surface of biliary epithelium. These data suggest that mechanisms involved in the pathophysiology of primary biliary cirrhosis may include both T cell and antibody mechanisms. The results also underscore the need to develop a functional, and not just a phenotypical, assay of the inflammatory infiltrate.

AB - The histological findings in patients with primary biliary cirrhosis have been well-defined and are often used in the clinical staging of disease. However, it has only been with the development of reagents that phenotypically characterize the lymphoid infiltrate that attempts have been made to correlate pathophysiology with immune effector populations. Indeed, the inflammatory hepatic lesions in primary biliary cirrhosis have been described as containing CD4-positive and CDS-positive T cells. Less clear, however, have been the T cell receptors in these lesions. Further, the data on immunoglobulin deposits in hepatic lesions have been less well-defined; this deficit may be a result of the quality of polyspecific sera and difficulties in background. To address these issues, we have used a battery of well-defined monospecific and polyspecific reagents to phenotypically define the occurrence of lymphoid cells in the livers of patients undergoing transplantation. Furthermore, we have defined these same markers on T cell lines derived from liver, regional lymph node and peripheral blood. The predominant cell type in the mononuclear infiltrate is the CD3+, CD4+ T lymphocyte bearing the T cell receptor αβ. T cell lines from the same patients demonstrate similar findings. Of special importance, however, was the detection of CD20+ B cells and Ig+ cells in the lymphoid infiltrate. Indeed, we also readily demonstrated the presence of immunoglobulin on the surface of biliary epithelium. These data suggest that mechanisms involved in the pathophysiology of primary biliary cirrhosis may include both T cell and antibody mechanisms. The results also underscore the need to develop a functional, and not just a phenotypical, assay of the inflammatory infiltrate.

UR - http://www.scopus.com/inward/record.url?scp=0025024248&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025024248&partnerID=8YFLogxK

M3 - Article

VL - 12

SP - 306

EP - 313

JO - Hepatology

JF - Hepatology

SN - 0270-9139

IS - 2

ER -